Results 11 to 20 of about 13,871 (203)
Human Hypoxanthine Phosphoribosyltransferase [PDF]
Journal of Biological Chemistry, 1969 Hypoxanthine phosphoribosyltransferase (inosine monophosphate:pyrophosphate phosphoribosyltransferase EC 2.4.2.8) has been purified 50-fold from an acid-treated lysate of human erythrocytes. The average particle weight of the enzyme was estimated by gel filtration at 60,000.
Rhoda Papaioannou +2 more
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Purification and characterization of mouse hypoxanthine-guanine phosphoribosyltransferase.
Journal of Biological Chemistry, 1975 Hypoxanthine-guanine phosphoribosyltransferase (HGPR transferase) (EC 2.4.2.8) has been purified approximately 4500-fold to apparent homogeneity from mouse liver. The procedure involves the use of affinity chromatography and was designed to be readily adaptable to small scale isolations.
S. H. Hughes +2 more
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Kinetic Studies of Hypoxanthine-Guanine Phosphoribosyltransferase
Journal of Biological Chemistry, 1968 Abstract The mechanism of reaction of human erythrocyte hypoxanthine-guanine phosphoribosyltransferase was investigated by initial velocity, product inhibition, and isotope exchange studies. Although initial velocity data are compatible with a mechanism involving binary enzyme-substrate complexes, the product inhibition and isotope exchange studies ...
Frederick M. Rosenbloom +4 more
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Crystal structure of Leishmania tarentolae hypoxanthine-guanine phosphoribosyltransferase [PDF]
BMC Structural Biology, 2007 Abstract Background Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) (EC 2.4.2.8) is a central enzyme in the purine recycling pathway. Parasitic protozoa of the order Kinetoplastida cannot synthesize purines de novo and use the salvage pathway to synthesize purine bases, making this an attractive target for ...
Otavio Henrique Thiemann +3 more
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Prioritization of Deleterious Variations in the Human Hypoxanthine-Guanine Phosphoribosyltransferase Gene [PDF]
Medical Laboratory Journal, 2018 Background and Objectives: Non-synonymous single nucleotide polymorphisms are typical genetic variations that may potentially affect the structure or function of expressed proteins, and therefore could be involved in complex disorders.
Semira Kheiri +3 more
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Human Hypoxanthine-Guanine Phosphoribosyltransferase
Journal of Biological Chemistry, 1971 Abstract Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) has been purified to homogeneity from human erythrocytes obtained from one male donor. The normal human enzyme has a Stokes radius of 36 A with a molecular weight of 68,000 and is composed of two subunits which have identical molecular weight and net charge.
William J. Arnold, William N. Kelley
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Hypoxanthine-Guanine Phosphoribosyltransferase Is Dispensable for Mycobacterium smegmatis Viability
Journal of Bacteriology, 2020 Purine bases, released by the hydrolytic and phosphorolytic degradation of nucleic acids and nucleotides, can be salvaged and recycled. The hypoxanthine-guanine phosphoribosyltransferase (HGPRT), which catalyzes the formation of guanosine-5′-monophosphate from guanine and inosine-5′-monophosphate from hypoxanthine, represents a potential ...
Knejzlík, Z. (Zdeněk) +3 more
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Research Advances in Purine Phosphoribosyltransferases of Protozoan Parasites
Guangdong nongye kexue, 2023 Parasitic protozoa are single-celled organisms that have adapted to live in cells of humans and animals. The protozoan parasites include Leishmania spp., Trypanosoma spp., Plasmodium spp., Toxoplasma gondii, Cryptosporidium spp., and Eimeria spp., which ...
Zhihui YU +7 more
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Analysis of parameters of purine metabolism in patients with diabetes mellitus type 2
Medičnì Perspektivi, 2023 The aim of this research is to comprehensively analyze the current state of purine metabolism as well as assess its features in patients with diabetes mellitus type 2, taking into account the clinical and metabolic polymorphism of the disease.
A.O. Chernyaeva +2 more
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Targeted Knock-in of Transgenes into the CHO Cell Genome Using CRISPR-mediated Integration Systems [PDF]
MATEC Web of Conferences, 2021 Biopharmaceutical proteins are usually produced by culturing recombinant Chinese hamster ovary (CHO) cells. High producer cell lines are screened from transfected cells with random integration of target genes. Since transgene expression is susceptible to
Iwao Ryusei +4 more
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