Results 41 to 50 of about 51,129 (240)
Frontiers in Veterinary Science, 2023 Quantitative polymerase chain reaction (qPCR) is an important method to detect gene expression at the molecular level. The selection of appropriate housekeeping genes is the key to accurately calculating the expression level of target genes and ...
Fei Shui +13 more
doaj +1 more source
Elevated Expression of Hypoxanthine Guanine Phosphoribosyltransferase within Malignant Tissue
, 2017 Hypoxanthine Guanine Phosphoribosyltransferase (HPRT) is a housekeeping enzyme involved in the purine synthesis of guanine and inosine in the salvage pathway.
M. Townsend +5 more
semanticscholar +1 more source
BioTechniques, 1999 A lacZ transgene, expressed by the myogenin promoter, was introduced into the mouse hypoxanthine phosphoribosyltransferase (Hprt) locus by gene targeting in embryonic stem cells.
Jay L. Vivian +2 more
doaj +1 more source
Scientific Reports, 2016 Human African Trypanosomiasis (HAT) is a life-threatening infectious disease caused by the protozoan parasite, Trypanosoma brucei (Tbr). Due to the debilitating side effects of the current therapeutics and the emergence of resistance to these drugs, new ...
D. Terán +6 more
semanticscholar +1 more source
Journal of Inborn Errors of Metabolism and Screening, 2021 Hypoxanthine-guanine phosphoribosyltransferase (HPRT) deficiency is a disorder of purine metabolism responsible for Lesch-Nyhan Disease (LND) and its variants, HPRT-related hyperuricemia with neurologic dysfunction (HND) and HPRT-related hyperuricemia ...
Laura E. Laróvere +6 more
doaj +1 more source
Proteomes of Lactobacillus delbrueckii subsp. bulgaricus LBB.B5 Incubated in Milk at Optimal and Low Temperatures. [PDF]
, 2017 We identified the proteins synthesized by Lactobacillus delbrueckii subsp. bulgaricus strain LBB.B5 in laboratory culture medium (MRS) at 37°C and milk at 37 and 4°C.
Angel Angelov +6 more
core +2 more sources
Identification of Fis1 Interactors in Toxoplasma gondii Reveals a Novel Protein Required for Peripheral Distribution of the Mitochondrion [PDF]
, 2020 Toxoplasma gondii’s single mitochondrion is very dynamic and undergoes morphological changes throughout the parasite’s life cycle. During parasite division, the mitochondrion elongates, enters the daughter cells just prior to cytokinesis, and undergoes ...
Arrizabalaga, Gustavo +2 more
core +1 more source
dGTP starvation in Escherichia coli provides new insights into the thymineless-death phenomenon.
PLoS Genetics, 2014 Starvation of cells for the DNA building block dTTP is strikingly lethal (thymineless death, TLD), and this effect is observed in all organisms. The phenomenon, discovered some 60 years ago, is widely used to kill cells in anticancer therapies, but many ...
Mark Itsko, Roel M Schaaper
doaj +1 more source
Targeting purine metabolism in ovarian cancer
Journal of Ovarian Research, 2022 Purine, an abundant substrate in organisms, is a critical raw material for cell proliferation and an important factor for immune regulation. The purine de novo pathway and salvage pathway are tightly regulated by multiple enzymes, and dysfunction in ...
Jingchun Liu +7 more
doaj +1 more source
The MAO-B Inhibitor Selegiline Reduces the Viability of Different Prostate Cancer Cell Lines and Enhances the Effects of Anti-Androgen and Cytostatic Agents. [PDF]
Pharmacol Res PerspectDistinct mechanisms of viability decreasing actions of selegiline in androgen‐insensitive PC3 and sensitive 22Rv1 PAC cell lines. MAO‐B inhibition in the mitochondrial membrane of PC3 cells leads to the upregulation of the BAX and downregulation of FOX and GLUT1 which potentially results in reduced glucose uptake and apoptosis induction.
Steib A +8 more
europepmc +2 more sources