Results 81 to 90 of about 51,129 (240)

Efficient cDNA cloning by direct phenotypic correction of a mutant human cell line (HPRT-) using an Epstein-Barr virus derived cDNA expression vector. [PDF]

, 1991
Human cells are, in general, poor recipients of foreign DNA, which has severely hampered the cloning of genes by direct phenotypic correction of deficient human cell lines after DNA mediated gene transfer. In this communication a methodology is presented
Backendorf, C.M.P. (Claude), Belt, P.B.G.M., Hoeijmakers, J.H.J. (Jan), Jongmans, W., Putte, P. (Pieter) van de, Wit, J. (Jan) de   +5 more
core   +2 more sources

The fate of thiopurine metabolites after switching to low‐dose thiopurine with allopurinol or thioguanine in IBD patients: A retrospective analysis

British Journal of Clinical Pharmacology, Volume 91, Issue 10, Page 2865-2873, October 2025.
Aims Shunting (hypermethylating) thiopurine metabolism, characterized by excessive 6‐MMPR production and (sub)therapeutic 6‐TGN levels, poses a significant challenge in the treatment of inflammatory bowel disease (IBD). This study evaluates the metabolic outcomes of switching to low‐dose thiopurine with allopurinol (LDTA) or thioguanine (TG) in IBD ...
Ahmed B. Bayoumy, Hedwig M. A. D'Agnolo, Nanne K. H. de Boer, Chris J. J. Mulder, Luc J. J. Derijks   +4 more
wiley   +1 more source

Human naive CD8 T cells down-regulate expression of the WNT pathway transcription factors lymphoid enhancer binding factor 1 and transcription factor 7 (T cell factor-1) following antigen encounter in vitro and in vivo [PDF]

, 2006
The transcription factors lymphoid enhancer binding factor 1 (LEF1) and transcription factor 7 (TCF7) (T cell factor-1 (TCF-1)) are downstream effectors of the WNT signaling pathway, which is a critical regulator of T cell development in the thymus.
Callan, Margaret F C, Freeman, Tom, Hasegawa, Hitoshi, Herbert, Mark, McMichael, Andrew J, Willinger, Tim   +5 more
core   +1 more source

Hypoxanthine Phosphoribosyltransferase (HPRT)-deficiency is associated with impaired fertility in the female rat [PDF]

, 2020
The purine hypoxanthine plays important role in regulating oocyte maturation and early embryonic development. The enzyme hypoxanthine phosphoribosyltransferase (HPRT) recycles hypoxanthine to generate substrates for nucleotide synthesis and key ...
Binas, Bert, Burdon, Tom, Clinton, Mike, Meek, Stephen, Sturmey, Roger, Sutherland, Linda, Wei, Jun   +6 more
core   +2 more sources

Postprandial Metabolism, Inflammation, and Plasma Bile Acid Kinetics in a Rat Model: Implications for Translational Research

Molecular Nutrition &Food Research, Volume 69, Issue 20, October 2025.
Sprague‐Dawley rats were used to investigate postprandial metabolic and inflammatory responses following the consumption of an energy‐dense meal. Time‐resolved plasma and liver analyses revealed fluctuations in bile acid levels, particularly cholic acid, along with changes in metabolic intermediates, oxylipins, and inflammatory markers.
Larissa Rodrigues, Carlos M. Donado‐Pestana, Tushar More, Mar Garcia‐Aloy, Gustavo M. Carneiro de Lima, Lucas X. Martins de Oliveira, Urska Vrhovsek, Karsten Hiller, Jarlei Fiamoncini   +8 more
wiley   +1 more source

Hypoxanthine-guanine phosphoribosyltransferase and inosine 5’-monophosphate dehydrogenase activities in three mammalian species: aquatic (Mirounga angustirostris), semiaquatic (Lontra longicaudis annectens) and terrestrial (Sus scrofa)

Frontiers in Physiology, 2015
Aquatic and semiaquatic mammals have the capacity of breath hold (apnea) diving. Northern elephant seals (Mirounga angustirostris) have the ability to perform deep and long duration dives; during a routine dive, adults can hold their breath for 25 min ...
Myrna eBarjau Perez-Milicua, Tania eZenteno-Savin, Daniel E. Crocker, Juan Pablo Gallo   +3 more
doaj   +1 more source

Lesch-Nyhan Syndrome: Evaluation of a Modified Bite Device to Prevent Bite Injuries

Applied Sciences, 2020
Lesch-Nyhan syndrome (LNS) is a serious form of hypoxanthine-guanine phosphoribosyltransferase (HPRT) deficiency, a hereditary purine metabolism disorder. The prevalence reported in the literature is 1/380,000 to 235,000 births.
Gaetano Ierardo, Valeria Luzzi, Gian Luca Sfasciotti, Antonella Polimeni, Maurizio Bossù   +4 more
doaj   +1 more source

Lesch-Nyhan syndrome: A case report

Journal of Indian Society of Pedodontics and Preventive Dentistry, 2008
Lesch-Nyhan syndrome, a rare inborn error of metabolism, is characterized by mental retardation and self-destructive behavior resulting in self-mutilation through biting and scratching.
Kale A, Shah K, Hallikerimath S
doaj  

Natural Products from Mexican Medicinal Plants as Promising Trypanocidal Drugs

ChemistrySelect, Volume 10, Issue 37, October 6, 2025.
Chagas disease (American Trypanosomiasis) is caused by the protozoan Trypanosoma cruzi. Worldwide it is one of the seventeen neglected tropical diseases. There is a need of new drugs. This review assesses the literature (2012‐2024) of secondary metabolites isolated from Mexican plants active against this parasite.
Karla Daniela Rodríguez‐Hernández, Elia Guadalupe Jaimes‐Castelán, Ricardo Reyes‐Chilpa   +2 more
wiley   +1 more source

Efficient gene targeting by homologous recombination in rat embryonic stem cells.

PLoS ONE, 2010
The rat is the preferred experimental animal in many biological studies. With the recent derivation of authentic rat embryonic stem (ES) cells it is now feasible to apply state-of-the art genetic engineering in this species using homologous recombination.
Stephen Meek, Mia Buehr, Linda Sutherland, Alison Thomson, John J Mullins, Andrew J Smith, Tom Burdon   +6 more
doaj   +1 more source