Results 1 to 10 of about 700,365 (275)

Cross-Platform Evaluation of Established NGS-Based Metabarcoding Methods for Detecting Food Fraud in Pistachio Products [PDF]

Foods
Next Generation Sequencing is a constantly evolving technology whose applicability is increasingly expanding into the field of routine food analysis.
Sina Rammouz, Jochen Riehle, Ansgar Ferner, Markus Fischer, Christian Schäfers   +4 more
doaj   +2 more sources

Comparative Meta-Analysis of Long-Read and Short-Read Sequencing for Metagenomic Profiling of the Lower Respiratory Tract Infections [PDF]

Microorganisms
Metagenomic next-generation sequencing (mNGS) is increasingly employed for the diagnosis of lower respiratory tract infections (LRTIs). However, the relative diagnostic performance of long-read versus short-read sequencing platforms remains incompletely ...
Giovanni Lorenzin, Maddalena Carlin
doaj   +2 more sources

Illuminating Choices for Library Prep: A Comparison of Library Preparation Methods for Whole Genome Sequencing of Cryptococcus neoformans Using Illumina HiSeq. [PDF]

, 2014
The industry of next-generation sequencing is constantly evolving, with novel library preparation methods and new sequencing machines being released by the major sequencing technology companies annually.
A Adey, A McKenna, BJ Loftus, EL van Dijk, GA Van der Auwera, H Li, H Li, H Li, J Dabney, JD McPherson, Johanna Rhodes, Kirsten Nielsen, L DeFrancesco, M Eisenstein, MA DePristo, MA Quail, Mathew A. Beale, Matthew C. Fisher, R Marine   +18 more
core   +24 more sources

Correcting Illumina data [PDF]

Briefings in Bioinformatics, 2014
Next-generation sequencing technologies revolutionized the ways in which genetic information is obtained and have opened the door for many essential applications in biomedical sciences. Hundreds of gigabytes of data are being produced, and all applications are affected by the errors in the data. Many programs have been designed to correct these errors,
Michael, Molnar, Lucian, Ilie
openaire   +2 more sources

Improved Protocols for Illumina Sequencing [PDF]

Current Protocols in Human Genetics, 2013
AbstractIn this unit, we describe a set of improvements that have been made to the standard Illumina protocols to make the sequencing process more reliable in a high‐throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data. Curr. Protoc. Hum. Genet. 79:18.2.1‐18.2.42.
Iraad F, Bronner, Michael A, Quail, Daniel J, Turner, Harold, Swerdlow   +3 more
openaire   +3 more sources

Ultra-deep sequencing validates safety of CRISPR/Cas9 genome editing in human hematopoietic stem and progenitor cells

Nature Communications, 2022
As CRISPR-based therapies enter the clinic, evaluation of safety remains a critical and active area of study. Here the authors use next generation sequencing to achieve high sequencing depth and demonstrate that clinically relevant delivery of high ...
M. Kyle Cromer, Valentin V. Barsan, Erich Jaeger, Mengchi Wang, Jessica P. Hampton, Feng Chen, Drew Kennedy, Jenny Xiao, Irina Khrebtukova, Ana Granat, Tiffany Truong, Matthew H. Porteus   +11 more
doaj   +1 more source

Illumina reads correction: evaluation and improvements

Scientific Reports, 2023
Abstract The paper focuses on the correction of Illumina WGS sequencing reads. We provide an extensive evaluation of the existing correctors. To this end, we measure an impact of the correction on variant calling (VC) as well as de novo assembly. It shows, that in selected cases read correction improves the VC results quality. We also
Maciej Długosz, Sebastian Deorowicz
openaire   +3 more sources

Bead-linked transposomes enable a normalization-free workflow for NGS library preparation

BMC Genomics, 2018
Background Transposome-based technologies have enabled the streamlined production of sequencer-ready DNA libraries; however, current methods are highly sensitive to the amount and quality of input nucleic acid.
Stephen Bruinsma, Joshua Burgess, Daniel Schlingman, Agata Czyz, Natalie Morrell, Catherine Ballenger, Heather Meinholz, Lee Brady, Anupama Khanna, Lindsay Freeberg, Rosamond G Jackson, Pascale Mathonet, Susan C Verity, Andrew F Slatter, Rooz Golshani, Haiying Grunenwald, Gary P Schroth, Niall A Gormley   +17 more
doaj   +1 more source

Whole-Genome Sequencing Can Identify Clinically Relevant Variants from a Single Sub-Punch of a Dried Blood Spot Specimen

International Journal of Neonatal Screening, 2023
The collection of dried blood spots (DBS) facilitates newborn screening for a variety of rare, but very serious conditions in healthcare systems around the world.
David J. McBride, Claire Fielding, Taksina Newington, Alexandra Vatsiou, Harry Fischl, Maya Bajracharya, Vicki S. Thomson, Louise J. Fraser, Pauline A. Fujita, Jennifer Becq, Zoya Kingsbury, Mark T. Ross, Stuart J. Moat, Sian Morgan   +13 more
doaj   +1 more source

The Diversity of REcent and Ancient huMan (DREAM): a new microarray for genetic anthropology and genealogy, forensics, and personalized medicine [PDF]

, 2017
The human population displays wide variety in demographic history, ancestry, content of DNA derived from hominins or ancient populations, adaptation, traits, copy number variation (CNVs), drug response, and more. These polymorphisms are of broad interest
Ainan I J Anderson, Dimitrios Arnellos, Eran Elhaik, Gregory Vilshansky, Gunes Ercal, illumina, illumina, illumina, illumina, Leeban Yusuf, Mehdi Pirooznia, Michael L Baird, Teresa Webster, Thermo Fisher Scientific, Thermo Fisher Scientific, Thermo Fisher Scientific, Umberto Esposito, Yontao Lu   +17 more
core   +1 more source