Results 21 to 30 of about 700,365 (275)
A Hybrid Sequencing Approach Completes the Genome Sequence of Thermoanaerobacter ethanolicus JW 200 [PDF]
, 2019 Thermoanaerobacter ethanolicus JW 200 has been identified as a potential sustainable biofuel producer due to its ability to readily ferment carbohydrates to ethanol. A hybrid sequencing approach, combining Oxford Nanopore and Illumina DNA sequence reads,
Ayine, Monica L. +9 more
core +1 more source
Nature Communications, 2022 Rapid diagnosis and implementation of treatments is crucial in many genetic conditions. Here the authors describe Genome-to-Treatment, a virtual disease management system that can achieve a rapid diagnosis by expedited whole genome sequencing in 13.5 ...
Mallory J. Owen +64 more
doaj +1 more source
An improved protocol for small RNA library construction using High Definition adapters [PDF]
, 2015 Next generation sequencing of small RNA (sRNA) libraries is widely used for studying sRNAs in various biological systems. However, cDNA libraries of sRNAs are biased for molecules that are ligated to adapters more or less efficiently than other molecules.
Aravin +28 more
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, 2022 Part three of the HTTM protocol. A low-cost and high-throughput Tn-seq protocol. This part cover the preparation of Illumina sequencing libraries form genomic DNA.
Antoine Champie, Amélie De Grandmaison
openaire +1 more source
Exploring genome wide bisulfite sequencing for DNA methylation analysis in livestock: a technical assessment [PDF]
, 2014 peer-reviewedRecent advances made in “omics” technologies are contributing to a revolution in livestock selection and breeding practices. Epigenetic mechanisms, including DNA methylation are important determinants for the control of gene expression in ...
Couldrey, Christine, Doherty, Rachael
core +2 more sources
BFC: correcting Illumina sequencing errors [PDF]
Bioinformatics, 2015 Abstract Summary: BFC is a free, fast and easy-to-use sequencing error corrector designed for Illumina short reads. It uses a non-greedy algorithm but still maintains a speed comparable to implementations based on greedy methods. In evaluations on real data, BFC appears to correct more errors with fewer overcorrections in comparison to ...
openaire +2 more sources
Monitoring Error Rates In Illumina Sequencing [PDF]
Journal of Biomolecular Techniques : JBT, 2016 Guaranteeing high-quality next-generation sequencing data in a rapidly changing environment is an ongoing challenge. The introduction of the Illumina NextSeq 500 and the depreciation of specific metrics from Illumina's Sequencing Analysis Viewer (SAV; Illumina, San Diego, CA, USA) have made it more difficult to determine directly the baseline error ...
Leigh J, Manley +2 more
openaire +2 more sources
Patient-reported outcomes associated with cancer screening: a systematic review
BMC Cancer, 2022 Background Multi-cancer early detection tests have been developed to enable earlier detection of multiple cancer types through screening. As reflected by patient-reported outcomes (PROs), the psychosocial impact of cancer screening is not yet clear.
Ashley Kim +3 more
doaj +1 more source
QQ-SNV: single nucleotide variant detection at low frequency by comparing the quality quantiles [PDF]
, 2015 Background: Next generation sequencing enables studying heterogeneous populations of viral infections. When the sequencing is done at high coverage depth ("deep sequencing"), low frequency variants can be detected.
Aerssens, Jeroen +7 more
core +4 more sources
HERVs establish a distinct molecular subtype in stage II/III colorectal cancer with poor outcome
npj Genomic Medicine, 2021 Colorectal cancer (CRC) is one of the most lethal malignancies. The extreme heterogeneity in survival rate is driving the need for new prognostic biomarkers.
Mahdi Golkaram +27 more
doaj +1 more source