DNA methylation profiling of primary neuroblastoma tumors using methyl-CpG-binding domain sequencing [PDF]
, 2016 Comprehensive genome-wide DNA methylation studies in neuroblastoma (NB), a childhood tumor that originates from precursor cells of the sympathetic nervous system, are scarce. Recently, we profiled the DNA methylome of 102 well-annotated primary NB tumors
Decock, Anneleen +4 more
core +1 more source
, 2022 Part three of the HTTM protocol. A low-cost and high-throughput Tn-seq protocol. This part cover the preparation of Illumina sequencing libraries form genomic DNA.
Antoine Champie, Amélie De Grandmaison
openaire +1 more source
Draft Genome Sequence of Photorhabdus luminescens subsp. laumondii HP88, an Entomopathogenic Bacterium Isolated from Nematodes [PDF]
, 2016 Photorhabdus luminescens subsp. laumondii HP88 is an entomopathogenic bacterium that forms a symbiotic association with Heterorhabditis nematodes. We report here a 5.27-Mbp draft genome sequence for P. luminescens subsp.
Abebe-Akele, Feseha +7 more
core +2 more sources
BFC: correcting Illumina sequencing errors [PDF]
Bioinformatics, 2015 Abstract Summary: BFC is a free, fast and easy-to-use sequencing error corrector designed for Illumina short reads. It uses a non-greedy algorithm but still maintains a speed comparable to implementations based on greedy methods. In evaluations on real data, BFC appears to correct more errors with fewer overcorrections in comparison to ...
openaire +2 more sources
Monitoring Error Rates In Illumina Sequencing [PDF]
Journal of Biomolecular Techniques : JBT, 2016 Guaranteeing high-quality next-generation sequencing data in a rapidly changing environment is an ongoing challenge. The introduction of the Illumina NextSeq 500 and the depreciation of specific metrics from Illumina's Sequencing Analysis Viewer (SAV; Illumina, San Diego, CA, USA) have made it more difficult to determine directly the baseline error ...
Leigh J, Manley +2 more
openaire +2 more sources
Patient-reported outcomes associated with cancer screening: a systematic review
BMC Cancer, 2022 Background Multi-cancer early detection tests have been developed to enable earlier detection of multiple cancer types through screening. As reflected by patient-reported outcomes (PROs), the psychosocial impact of cancer screening is not yet clear.
Ashley Kim +3 more
doaj +1 more source
HERVs establish a distinct molecular subtype in stage II/III colorectal cancer with poor outcome
npj Genomic Medicine, 2021 Colorectal cancer (CRC) is one of the most lethal malignancies. The extreme heterogeneity in survival rate is driving the need for new prognostic biomarkers.
Mahdi Golkaram +27 more
doaj +1 more source
Taxonomy of anaerobic digestion microbiome reveals biases associated with the applied high throughput sequencing strategies [PDF]
, 2018 In the past few years, many studies investigated the anaerobic digestion microbiome by means of 16S rRNA amplicon sequencing. Results obtained from these studies were compared to each other without taking into consideration the followed procedure for ...
Angelidaki, Irini +4 more
core +3 more sources
NxRepair: error correction in de novo sequence assembly using Nextera mate pairs [PDF]
PeerJ, 2015 Scaffolding errors and incorrect repeat disambiguation during de novo assembly can result in large scale misassemblies in draft genomes. Nextera mate pair sequencing data provide additional information to resolve assembly ambiguities during scaffolding ...
Rebecca R. Murphy +3 more
doaj +2 more sources
Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]
, 2018 All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane +2 more
core +2 more sources