Results 141 to 150 of about 142,852 (247)

Environmental DNA (eDNA) 12S metabarcoding Illumina MiSeq NGS PCR Protocol v1

, 2018
The 12S protocol is aimed at amplifying the hypervariable region of the mitochondrial DNA 12S rRNA gene in eukaryotes. The primers (MiFish-U-F & MiFish-U-R) used in this protocol were developed by Miya et al., 2015 for metabarcoding environmental DNA (eDNA) from fishes.
Collin Closek, Anni Djurhuus, Katie Pitz, Ryan Kelly, Reiko Michisaki, Kristine Walz, Hilary Starks, Francisco Chavez, Alexandria Boehm, Mya Breitbart   +9 more
openaire   +1 more source

Iron overload in hereditary spherocytosis: Are genetic factors the cause?

British Journal of Haematology, EarlyView.
Summary Non‐transfusional iron overload (IOL) in hereditary spherocytosis (HS) is poorly documented compared with other red blood cell disorders. We studied 13 HS adults with confirmed IOL to identify potential genetic factors. Using a next‐generation sequencing panel of 46 genes related to HS, anaemia and iron metabolism, we found no association ...
Lucie Donaty, Muriel Giansily‐Blaizot, Ivan Bertchansky, Séverine Cunat, Vincent Azoury, Perrine Mahe, Patricia Aguilar Martinez   +6 more
wiley   +1 more source

A Transcriptomic Analysis of Cancer‐Stromal Interactome in Lung Cancer Xenograft Models

Cancer Science, EarlyView.
We conducted a comprehensive analysis of the lung cancer interactome to identify key ligand–receptor pairs involved in the aggressiveness of lung adenocarcinoma. Tumor necrosis factor superfamily member 12 and its receptor tumor necrosis factor receptor superfamily member 12A signaling axis may be potential candidates for therapeutic intervention for ...
Yuriko Takayama‐Isagawa, Daisuke Komura, Takayuki Isagawa, Yusuke Amano, Atsushi Kihara, Tamaki Miura, Taichiro Yoshimoto, Hiroyoshi Tsubochi, Kazutaka Fujita, Koichi Hagiwara, Makoto Maemondo, Tetsuo Ushiku, Shumpei Ishikawa, Noriyoshi Fukushima, Kentaro Inamura, Daisuke Matsubara, Toshiro Niki   +16 more
wiley   +1 more source

A Walk in the Park: Influence of Natural Co‐Exposure to Grass Pollen and Fungal Spores on Nasal Mycobiome and Cytokine Responses

Clinical &Experimental Allergy, EarlyView.
Volunteers stayed 3 h indoors and followed 1 h outdoors in a flowering meadow. Nasal mycobiome was analysed and aeroallergen content measured in nasal filters. Airborne fungal spores were quantified and fungal isolates were sequenced. Isolated fungal spores were used for stimulation of nasal epithelial cells.
Annika Eggestein, Denise Rauer, Selina M. Herrmann, Franziska Kolek, Vivien Leier‐Wirtz, Swetlana Urban, Bärbel Foesel, Michael Schloter, Madhumita Bhattacharyya, Ioana Pyrri, Matthias Reiger, Vera Schwierzeck, Claudia Hülpüsch, Claudia Traidl‐Hoffmann, Athanasios Damialis, Stefanie Gilles   +15 more
wiley   +1 more source

Disentangling mechanisms that mediate soil fungal α and β diversity during forest secondary succession

Ecography, EarlyView.
Understanding the mechanisms controlling community diversity is a central, topic in ecology, particularly in microbial ecology. Although species pools and local assembly processes are believed to play non‐negligible roles in shaping the within‐community (α) and among‐community (β) diversity of microbial communities, their relative importance as ...
Xiao Zhang, Biao Dong, Yongtao Huang, Handan Dai, Zhu Yang, Shenglei Fu, Qiang Li, Yidan Yuan, Yuhua Tan, Huan Li, Shirong Liu   +10 more
wiley   +1 more source

Floral resource strips enhance parasitoid abundance and diversity in apple orchards and promote agroecological advances in a South African biosphere reserve

Insect Conservation and Diversity, EarlyView.
Floral resource strips within apple orchards increased parasitoid abundance and influenced community composition, enhancing local biodiversity in the Kogelberg Biosphere Reserve's transition zone. Ruderal and natural fynbos habitats supported higher parasitoid richness and abundance, emphasising the importance of conserving semi‐natural habitats ...
Fabrizia Ratto, Peter Steward, Steven M. Sait, Julien M. Haran, Rene Gaigher, James S. Pryke, Michael J. Samways, William Kunin   +7 more
wiley   +1 more source

Bacterial diversity and composition of alfalfa silage as analyzed by Illumina MiSeq sequencing: Effects of Escherichia coli O157:H7 and silage additives.

Journal of Dairy Science, 2017
I. Ogunade, Y. Jiang, A. P. Cervantes, Dong-Hyeon Kim, A. Oliveira, D. Vyas, Z. Weinberg, K. Jeong, A. Adesogan   +8 more
semanticscholar   +1 more source

Microbiome of Apical Intracanal and Extraradicular Biofilms From the Same Roots of Teeth With Persistent Apical Periodontitis: An Observational Study

International Endodontic Journal, EarlyView.
ABSTRACT Aim Bacterial biofilms around the apex are crucial in disease progression and persistence of apical periodontitis. While intracanal biofilms initiate infection, extraradicular biofilms contribute to treatment resistance and persistence. Thus, a comprehensive understanding of these biofilms may help elucidate mechanisms underlying persistent ...
Yoko Asahi, Nanako Kuriki, Motoki Okamoto, Daisuke Motooka, Shota Nakamura, Hazuki Maezono, Kittipit Klanliang, Tsuyoshi Shimaoka, Tetsuya Iida, Shigeyuki Ebisu, Yuichiro Noiri, Mikako Hayashi   +11 more
wiley   +1 more source

Enhancing fish community monitoring at a cabled observatory by combining environmental DNA and imaging analysis

Journal of Fish Biology, EarlyView.
Abstract Cabled multiparametric observatories are sustaining ecological monitoring by collecting long‐term real‐time biological and environmental data. Here, we investigated fish communities by sampling environmental DNA (eDNA) over 4 days near the multiparametric cabled video‐observatory OBSEA (Northwestern Mediterranean Sea).
Maddalena Tibone, Marco Francescangeli, Sergio Stefanni, Jacopo Aguzzi, Bernadette O'Neill, Joaquin Del Rio, Daniel Mihai Toma, Luca Mirimin   +7 more
wiley   +1 more source

Assessment of bacterial contamination of toothbrushes using Illumina MiSeq

Oral Biology Research, 2019
So Yeon Lee, Si Young Lee
openaire   +2 more sources