Results 141 to 150 of about 156,271 (269)

Species‐Specific Shrub Encroachment Drives Divergent Soil Fungal Assembly Processes and Network Structures in Alpine Meadows

Land Degradation &Development, EarlyView.
ABSTRACT Shrub encroachment is rapidly transforming alpine meadow ecosystems worldwide, yet its consequences for belowground microbial organization remain insufficiently understood, particularly when different shrub species exert contrasting ecological effects.
Yang Liu, Hong Xiao, Yuping Rong, Kuiju Niu, Kai Ma, Yun Wang, Xin Zhang, Linqian Song   +7 more
wiley   +1 more source

Comparative analysis of Illumina, PacBio, and nanopore for 16S rRNA gene sequencing of rabbit’s gut microbiota

Frontiers in Microbiomes
This research aims to assess whether sequencing the full length of the 16S rRNA gene using PacBio HiFi sequencing and Oxford Nanopore Technology (ONT) platforms outperform Illumina MiSeq platform in providing detailed specie level insights.
Iliyass Biada, María Antonia Santacreu, Oscar González-Recio, Noelia Ibáñez-Escriche   +3 more
doaj   +1 more source

Is DNA metabarcoding an option for formaldehyde‐preserved zooplankton time series?

Limnology and Oceanography: Methods, EarlyView.
Abstract The recovery of amplifiable DNA from formaldehyde‐fixed (FF) zooplankton samples has long been considered problematic, but advances in degraded‐DNA retrieval have renewed interest in FF samples. To access the information stored in long‐term zooplankton time series, we evaluated methods for extracting amplifiable DNA from community samples ...
Aitor Albaina, Anders Lanzén, Irati Miguel, Fernando Rendo, María Santos   +4 more
wiley   +1 more source

Cotton strips as a model substrate for analysis of leaf‐litter‐colonizing microbial assemblages in streams

Limnology and Oceanography: Methods, EarlyView.
Abstract Leaf‐litter decomposition is a key indicator of the functional integrity of stream ecosystems and cotton strips have been employed as a standard substrate for quantification of litter‐decomposition rates in streams across the globe. Cotton strips also offer a standardized method for assessing structural integrity of stream microbial ...
Trinity Shoemaker, Cynthia Nguyen, Scott D. Tiegs, David M. Costello, John J. Kelly   +4 more
wiley   +1 more source

Illumina Read Data

, 2014
Illumina MiSeq paired reads by individual for 44 individuals (40 Pseudacris, 2 Hyla cinerea, 2 Acris gryllus).
Lisa N. Barrow (3251613), Sandra A. Emme (3252450), Emily Moriarty Lemmon (3192092), Hannah F. Ralicki (3252453)   +3 more
core   +1 more source

Expanded ATXN3 CAG Repeat is Stable in Human Purkinje Cells

Movement Disorders, EarlyView.
Abstract Background Spinocerebellar ataxia type 3 (SCA3) is a neurodegenerative disorder caused by an abnormally long polyglutamine‐encoding CAG repeat in the ATXN3 gene. Objectives We aimed to determine whether somatic expansion of the mutant ATXN3 (mATXN3) CAG repeat is present in the output cell of the cerebellar cortex, the Purkinje cell (PC), in ...
Hasnahana Chetia, Laura Kus, Erika Sipos, Nathaniel Heintz, Kert Mätlik   +4 more
wiley   +1 more source

Evaluation of a custom QIAseq targeted DNA panel with 164 ancestry informative markers sequenced with the Illumina MiSeq. [PDF]

Sci Rep, 2021
Truelsen D, Freire-Aradas A, Nazari M, Aliferi A, Ballard D, Phillips C, Morling N, Pereira V, Børsting C.   +8 more
europepmc   +1 more source

Phosphorus limits the growth of bacterial and eukaryotic primary producers in recently deglaciated High Arctic soils

Oikos, EarlyView.
Glaciers are receding at unprecedented rates worldwide, exposing large areas of barren sediment that are quickly colonized by microbes. Nutrient availability is a critical driver of early ecosystem succession, but in the High Arctic its role has remained uncertain.
Steven K. Schmidt, Jannetta N. Robinson, Adam J. Solon, James A. Bradley, Anastasia S. Hambi, Rachel R. Rubin, Charles Oliver, Ben W. Johnson, Mihai O. Cimpoiasu, Trevor P. Irons, Oliver Kuras, Anna Bergstrom, Pacifica Sommers   +12 more
wiley   +1 more source

Whole-Genome Sequencing of SARS-CoV-2: Assessment of the Ion Torrent AmpliSeq Panel and Comparison with the Illumina MiSeq ARTIC Protocol. [PDF]

J Clin Microbiol, 2021
Plitnick J, Griesemer S, Lasek-Nesselquist E, Singh N, Lamson DM, St George K.   +5 more
europepmc   +1 more source

A scalable workflow for sensitive detection and genomic profiling of tumour cells in lymph nodes from FFPE tissue

The Journal of Pathology, EarlyView.
Abstract Accurate detection of lymph node (LN) metastases is critical for prognosis and surgical decision‐making in head and neck squamous cell carcinoma (HNSCC) and other epithelial cancers. Standard histopathology examines only a small fraction of LN tissue, limiting sensitivity for micrometastases and isolated tumour cells.
Tristan Bergmann, Marianne Hölbling, Monica Sudarsanam, Christiane Driemel, Maximilian E Daniels, Joanna Swierz, Sandra Jaschinski, Maximilian Seidl, Norbert R Kübler, Wolfram T Knoefel, Rui PL Neves, Karl C Sproll, Nikolas H Stoecklein   +12 more
wiley   +1 more source