Short read Illumina data for the de novo assembly of a non-model snail species transcriptome (Radix balthica, Basommatophora, Pulmonata), and a comparison of assembler performance [PDF]
, 2011 Background: Until recently, read lengths on the Solexa/Illumina system were too short to reliably assemble transcriptomes without a reference sequence, especially for non-model organisms.
Wheat, Christopher W. +10 more
core +2 more sources
Illuminating Choices for Library Prep: A Comparison of Library Preparation Methods for Whole Genome Sequencing of Cryptococcus neoformans Using Illumina HiSeq. [PDF]
, 2014 The industry of next-generation sequencing is constantly evolving, with novel library preparation methods and new sequencing machines being released by the major sequencing technology companies annually.
Mathew A Beale +14 more
core +1 more source
NGS4Mater.Sci.: Phage Sequence Analysis of Illumina Next Generation Sequencing Data [PDF]
, 2020 Das hinterlegte Skript ermöglicht die statistische Auswertung von Illumina Next Generation Hochdurchsatz-Sequenzierungsdaten aus Phagendisplay Screenings.The provided script enables the statistical evaluation of Illumina next generation high throughput ...
Juds, Carmen +2 more
core +1 more source
Population genetic analysis of Plasmodium falciparum parasites using a customized Illumina GoldenGate genotyping assay. [PDF]
, 2011 The diversity in the Plasmodium falciparum genome can be used to explore parasite population dynamics, with practical applications to malaria control.
Abdoulaye Djimde +137 more
core +1 more source
Large scale single nucleotide polymorphism discovery in unsequenced genomes using second generation high throughput sequencing technology: applied to turkey [PDF]
, 2009 Background The development of second generation sequencing methods has enabled large scale DNA variation studies at moderate cost. For the high throughput discovery of single nucleotide polymorphisms (SNPs) in species lacking a sequenced reference genome,
Kerstens Hindrik HD +21 more
core +1 more source
Plant Sequence Capture Optimised for Illumina Sequencing
BIO-PROTOCOL, 2014 Plant Sequence Capture is used for targeted resequencing of whole exomes (all exons of a genome) of complex genomes e.g. barley and its relatives (Mascher et al., 2013). Sequencing and computing costs are significantly reduced since only the greatly enriched and gene-coding part of the barley genome is targeted, that corresponds to only 1-2% of the ...
Axel Himmelbach +2 more
openaire +2 more sources
nifH amplification for Illumina sequencing v1 [PDF]
, 2020 For metabarcoding purpose, the first step involves the amplification by PCR of a given gene region (for example V4 or V9 region of 18S rRNA gene) or gene itself if its size does not exceed 600bp (the longest fragment size that can be sequenced by Illumina technology).
Estelle Bigeard +2 more
openaire +1 more source
Complete Genome Sequence of Neisseria musculi Using Illumina and PacBio Sequencing
Microbiology Resource Announcements, 2021 Neisseria musculi is an oral commensal of wild-caught mice. Here, we report the complete genome sequence of N. musculi strain NW831, generated using a combination of the Illumina and PacBio platforms.
Eliza Thapa +5 more
openaire +2 more sources
Insight into biases and sequencing errors for amplicon sequencing with the Illumina MiSeq platform [PDF]
Nucleic Acids Research, 2015 With read lengths of currently up to 2 × 300 bp, high throughput and low sequencing costs Illumina's MiSeq is becoming one of the most utilized sequencing platforms worldwide. The platform is manageable and affordable even for smaller labs. This enables quick turnaround on a broad range of applications such as targeted gene sequencing, metagenomics ...
Schirmer, Melanie +5 more
openaire +4 more sources