Results 21 to 30 of about 1,964,998 (260)
Illumina MiSeq sequencing disfavours a sequence motif in the GFP reporter gene [PDF]
Scientific Reports, 2016 AbstractGreen fluorescent protein (GFP) is one of the most used reporter genes. We have used next-generation sequencing (NGS) to analyse the genetic diversity of a recombinant influenza A virus that expresses GFP and found a remarkable coverage dip in the GFP coding sequence.
Van den Hoecke, Silvie +2 more
openaire +3 more sources
A Short Review on Illumina Sequencing Technology
Austin Journal of Veterinary Science & Animal Husbandry, 2022 DNA sequencing is that the method of crucial the supermolecule sequence – the order of nucleotides in deoxyribonucleic acid. It includes any methodology or technology that’s accustomed verify the order of the four bases: A, guanine, cytosine, and T. Illumina technology is sequencing by synthesis approach and is presently the foremost used technology ...
openaire +1 more source
Illumina sequence data summary.
, 2016 Illumina sequence data summary.
Shea N. Gardner (11724) +10 more
core +1 more source
Summary of sequence assembly after Illumina sequence.
, 2015 Summary of sequence assembly after Illumina sequence.
Chi-Chu Tsai (668824) +6 more
core +1 more source
Finding novel vulnerabilities of hypomorphic BRCA1 alleles
Molecular Oncology, EarlyView.Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder +10 more
wiley +1 more source
A Comparison of Base-calling Algorithms for Illumina Sequencing Technology [PDF]
Briefings in Bioinformatics, 2015 Recent advances in next-generation sequencing technology have yielded increasing cost-effectiveness and higher throughput produced per run, in turn, greatly influencing the analysis of DNA sequences. Among the various sequencing technologies, Illumina is by far the most widely used platform.
Ashley Cacho +3 more
openaire +3 more sources
Diagnostic applications of next generation sequencing: working towards quality standards [PDF]
, 2012 Over the past 6 years, next generation sequencing (NGS) has been established as a valuable high-throughput method for research in molecular genetics and has successfully been employed in the identification of rare and common genetic variations. All major
Klein, Hanns-Georg +27 more
core +1 more source
MITF maintains genome stability in nonmelanocyte lineages
Molecular Oncology, EarlyView.MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir +13 more
wiley +1 more source
Microbiome Profiling by Illumina Sequencing of Combinatorial Sequence-Tagged PCR Products
PLoS ONE, 2010 28 pages, 13 ...
Gloor, Gregory B. +6 more
openaire +6 more sources
A large genome center's improvements to the Illumina sequencing system [PDF]
Nature Methods, 2008 The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average ...
Michael A, Quail +7 more
openaire +2 more sources