Results 61 to 70 of about 420,786 (307)
Environmental DNAAdvancements in sequencing technologies have revolutionized environmental research, leading to a superior understanding of ecosystem functions. In recent years, high‐throughput sequencing techniques evolved from short‐read Illumina sequencing to long ...
Dana Bludau +5 more
doaj +1 more source
Nanopore duplex sequencing as an alternative to Illumina MiSeq sequencing for eDNA-based biomonitoring of coastal aquaculture impacts [PDF]
Metabarcoding and MetagenomicsOxford Nanopore Technologies has recently launched a duplex sequencing strategy and announced an improved error rate which is in a similar order of magnitude as Illumina sequencing.
Thorsten Stoeck +3 more
doaj +3 more sources
ATF4‐mediated stress response as a therapeutic vulnerability in chordoma
Molecular Oncology, EarlyView.We screened 5 chordoma cell lines against 100+ inhibitors of epigenetic and metabolic pathways and kinases and identified halofuginone, a tRNA synthetase inhibitor. Mechanistically halofuginone induces an integrated stress response, with eIF2alpha phosphorylation, activation of ATF4 and its target genes CHOP, ASNS, INHBE leading to cell death ...
Lucia Cottone +11 more
wiley +1 more source
Illumina-based transcriptome sequencing of mussel Mytilus coruscus mantle
浙江大学学报. 农业与生命科学版, 2015 Mollusk shell is an exoskeleton made up of organic mineral particles and its function is to protect the inner soft body from predators and environmental damage. Due to its superior mechanical properties, such as stiffness, fracture toughness, and tensile
Bao Linfei +5 more
doaj +1 more source
Plecstatin inhibits hepatocellular carcinoma tumorigenesis and invasion through cytolinker plectin
Molecular Oncology, EarlyView.The ruthenium‐based metallodrug plecstatin exerts its anticancer effect in hepatocellular carcinoma (HCC) primarily through selective targeting of plectin. By disrupting plectin‐mediated cytoskeletal organization, plecstatin inhibits anchorage‐dependent growth, cell polarization, and tumor cell dissemination.
Zuzana Outla +10 more
wiley +1 more source
stilPCR increases the effective sequencing length of Illumina targeted next-generation sequencing.
PLoS ONEIdentifying pathogens, resistance-conferring mutations, and strain types through targeted amplicon sequencing is an important tool. However, due to the limitations of short read sequencing, many applications require the division of limited clinical ...
Jason D Limberis +9 more
doaj +1 more source
npj Science of Food, 2021 Efficient and accurate identification of microorganisms throughout the food chain can potentially allow the identification of sources of contamination and the timely implementation of control measures.
Aoife J. McHugh +5 more
doaj +1 more source
nCoV-2019 sequencing protocol for illumina v1 [PDF]
, 2020 This protocol is folked from "ARTIC amplicon sequencing protocol for MinION for nCoV-2019" by Josh Quick to adapt it to illumina sequencers. Because the PCR products are fragmented and ligated with adapters, this protocol is not restricted to 250 PE mode of MiSeq.
Kentaro Itokawa +4 more
openaire +1 more source
Therapeutic strategies for MMAE‐resistant bladder cancer through DPP4 inhibition
Molecular Oncology, EarlyView.We established monomethyl auristatin E (MMAE)‐resistant bladder cancer (BC) cell lines by exposure to progressively increasing concentrations of MMAE in vitro. RNA sequencing showed DPP4 expression was increased in MMAE‐resistant BC cells. Both si‐DPP4 and the DPP4 inhibitor sitagliptin suppressed the viability of MMAE‐resistant BC cells.
Gang Li +10 more
wiley +1 more source
CRISPR-hybrid N8 Illumina sequencing data
, 2023 FASTQ files used in data analysis for "CRISPR-Hybrid: A CRISPR-Mediated Intracellular Directed Evolution Platform for RNA Aptamers".
openaire +1 more source