Compressed sensing expands the multiplexity of imaging mass cytometry. [PDF]
Nat Commun, 2023 AbstractThe multiplexity of current antibody-based imaging is limited by the number of reporters that can be detected simultaneously. Compressed sensing can be used to recover high-dimensional information from low-dimensional measurements when the data has a structure that allows sparse representation. Previously, in composite in situ imaging (CISI) of
Hosogane T +4 more
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Using Imaging Mass Cytometry to Define Cell Identities and Interactions in Human Tissues. [PDF]
Front Physiol, 2021 In the evolving landscape of highly multiplexed imaging techniques that can be applied to study complex cellular microenvironments, this review characterizes the use of imaging mass cytometry (IMC) to study the human kidney.
Kakade VR, Weiss M, Cantley LG.
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Methods for analyzing tellurium imaging mass cytometry data
PLOS ONE, 2019 Imaging mass cytometry (IMC) is a technique allowing visualization and quantification of over 40 biological parameters in a single experiment with subcellular spatial resolution, however most IMC experiments are limited to endpoint analysis with antibodies and DNA stains.
Jay Bassan, Mark Nitz
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Adaptation of Imaging Mass Cytometry to Explore the Single Cell Alloimmune Landscape of Liver Transplant Rejection. [PDF]
Front Immunol, 2022 Rejection continues to be an important cause of graft loss in solid organ transplantation, but deep exploration of intragraft alloimmunity has been limited by the scarcity of clinical biopsy specimens.
Ung N +16 more
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Application of High-Throughput Imaging Mass Cytometry Hyperion in Cancer Research. [PDF]
Front Immunol, 2022 Imaging mass cytometry (IMC) enables the in situ analysis of in-depth-phenotyped cells in their native microenvironment within the preserved architecture of a single tissue section. To date, it permits the simultaneous analysis of up to 50 different protein- markers targeted by metal-conjugated antibodies.
Le Rochais M, Hemon P, Pers JO, Uguen A.
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An imaging mass cytometry immunophenotyping panel for non-human primate tissues. [PDF]
Front Immunol, 2022 It has recently become clear that spatial organization contributes to cellular function and that expanding our knowledge on cellular organization is essential to further our understanding of processes in health and disease. Imaging mass cytometry enables high dimensional imaging of tissue while preserving spatial context and is therefore a suitable ...
Niewold P +4 more
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Multiplexed imaging mass cytometry reveals distinct tumor-immune microenvironments linked to immunotherapy responses in melanoma. [PDF]
Commun Med (Lond), 2022 Xiao, Guo et al. use imaging mass cytometry to evaluate the spatial composition of the tumor microenvironment in melanoma. The authors identify features of the microenvironment associated with response to anti-PD-1 immunotherapy.
Xiao X +10 more
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High-resolution imaging mass cytometry to map subcellular structures. [PDF]
Nat MethodsBollhagen A +5 more
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Dual-modality imaging of immunofluorescence and imaging mass cytometry for whole-slide imaging and accurate segmentation. [PDF]
Cell Rep Methods, 2023 Summary: Imaging mass cytometry (IMC) is a powerful technique capable of detecting over 30 markers on a single slide. It has been increasingly used for single-cell-based spatial phenotyping in a wide range of samples.
Kim EN +10 more
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Flow Cytometry II: Mass and Imaging Cytometry [PDF]
Journal of Investigative Dermatology, 2015 Correction to: Journal of Investigative Dermatology (2015) 135, e36; doi:10.1038/id.2015 ...
Hung Q. Doan +2 more
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