Results 21 to 30 of about 195,947 (313)

Oligonucleotide-polymer conjugation for imaging mass cytometry v2 [PDF]

, 2023
This protocol describes the procedures to label 5'-thiol modified oligonucleotides with maleimide carrying polymers which are also chelated with metal-ions. This is the basis to perform RNAscope with Imaging Mass Cytometry since therein we use metal readouts instead of fluorescence readouts. This protocol has been used in the following publications:
Danielschulz not provided
openalex   +2 more sources

From surfing to diving into the tumor microenvironment through multiparametric imaging mass cytometry. [PDF]

Front Immunol
The tumor microenvironment (TME) is a complex ecosystem where malignant and non-malignant cells cooperate and interact determining cancer progression. Cell abundance, phenotype and localization within the TME vary over tumor development and in response ...
Erreni M, Fumagalli MR, Marozzi M, Leone R, Parente R, D'Anna R, Doni A.   +6 more
europepmc   +2 more sources

Imaging mass cytometry dataset of small-cell lung cancer tumors and tumor microenvironments. [PDF]

BMC Res Notes
Objectives Small cell lung cancer (SCLC) accounts for approximately 15% of lung tumors and is marked by aggressive growth and early metastatic spread. In this study, we used two SCLC mouse models with differing tumor mutation burdens (TMB).
Rose F, Ibruli O, Lichius L, Kiljan M, Gozum G, Caiaffa MI, Cai J, Niu LN, Herter JM, Grüll H, Büttner R, Beleggia F, Bosco G, George J, Herter-Sprie GS, Reinhardt HC, Bozek K.   +16 more
europepmc   +2 more sources

YOUPI: Your powerful and intelligent tool for segmenting cells from imaging mass cytometry data. [PDF]

Front Immunol, 2023
The recent emergence of imaging mass cytometry technology has led to the generation of an increasing amount of high-dimensional data and, with it, the need for suitable performant bioinformatics tools dedicated to specific multiparametric studies.
Scuiller Y, Hemon P, Le Rochais M, Pers JO, Jamin C, Foulquier N.   +5 more
europepmc   +2 more sources

Imaging mass cytometry reveals generalised deficiency in OXPHOS complexes in Parkinson's disease. [PDF]

NPJ Parkinsons Dis, 2021
Here we report the application of a mass spectrometry-based technology, imaging mass cytometry, to perform in-depth proteomic profiling of mitochondrial complexes in single neurons, using metal-conjugated antibodies to label post-mortem human midbrain ...
Chen C, McDonald D, Blain A, Sachdeva A, Bone L, Smith ALM, Warren C, Pickett SJ, Hudson G, Filby A, Vincent AE, Turnbull DM, Reeve AK.   +12 more
europepmc   +2 more sources

Coupling imaging mass cytometry with Alcian blue histochemical staining for a single-slide approach. [PDF]

Front Immunol
Imaging mass cytometry (IMC) is a metal mass spectrometry-based method allowing highly multiplex immunophenotyping of cells within tissue samples. However, some limitations of IMC are its 1-µm resolution and its time and costs of analysis limiting ...
Hemon P, Ben-Guigui D, Geier M, Castillon M, Paranthoen C, Pers JO, Le Rochais M, Uguen A.   +7 more
europepmc   +2 more sources

Pan-cancer convergence of tumour-immune microenvironment motifs revealed by CyTOF and imaging mass cytometry. [PDF]

Front Immunol
Mass cytometry (CyTOF) and Imaging Mass Cytometry (IMC) provide single-cell resolution for over 50 protein markers, enabling unprecedented exploration of tumour and immune heterogeneity.
Vallée A, Drezet A, Arutkin M.
europepmc   +2 more sources

Imaging mass cytometry reveals the prominent role of myeloid cells at the maternal-fetal interface. [PDF]

iScience, 2022
Summary: Although the immunological complexity of the maternal-fetal interface is well appreciated, the actual interaction of maternal immune cells and fetal trophoblasts is insufficiently understood. To comprehend the composition and spatial orientation
Krop J, van der Zwan A, Ijsselsteijn ME, Kapsenberg H, Luk SJ, Hendriks SH, van der Keur C, Verleng LJ, Somarakis A, van der Meeren L, Haasnoot G, Bos M, de Miranda NFCC, Chuva de Sousa Lopes SM, van der Hoorn MP, Koning F, Claas FHJ, Heidt S, Eikmans M.   +18 more
europepmc   +2 more sources

Multiplexed Imaging Mass Cytometry Analysis in Preclinical Models of Pancreatic Cancer. [PDF]

Int J Mol Sci
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers. PDAC is characterized by a complex tumor microenvironment (TME), that plays a pivotal role in disease progression and resistance to therapy. Investigating the spatial distribution and interaction of TME cells with the tumor is the basis for understanding the mechanisms ...
Erreni M, Fumagalli MR, Zanini D, Candiello E, Tiberi G, Parente R, D'Anna R, Magrini E, Marchesi F, Cappello P, Doni A.   +10 more
europepmc   +3 more sources

Dual-modality imaging of immunofluorescence and imaging mass cytometry for whole slide imaging with accurate single-cell segmentation [PDF]

, 2023
Kim EN, Chen PZ, Bressan D, Tripathi M, Miremadi A, di Pietro M, Coussens LM, Hannon GJ, Fitzgerald RC, Zhuang L, Chang YH.   +10 more
europepmc   +2 more sources