Results 61 to 70 of about 195,947 (313)

Delivery of mixed-lineage kinase domain-like protein by vapor nanobubble photoporation induces necroptotic-like cell death in tumor cells [PDF]

, 2019
Modern molecular medicine demands techniques to efficiently deliver molecules directly into mammalian cells. As proteins are the final mediators of most cellular pathways, efficient intracellular protein delivery techniques are highly desired.
Braeckmans, Kevin, Brans, Toon, Declercq, Wim, Fraire, Juan, Raemdonck, Koen, Raes, Laurens, Roelandt, Ria, Saelens, Xavier, Stremersch, Stephan, Van Hoecke, Lien, Vandenabeele, Peter   +10 more
core   +2 more sources

Imaging Mass Cytometry Antibody Staining v1

, 2020
The following SOP describes procedures required for the antibody staining of formalin fixed, paraffin-embedded (FFPE) samples. In this SOP, the term “samples” refers to FFPE tissue samples.
Michelle Daniel, Marda Jorgensen
openaire   +1 more source

Metal Isotope-Tagged Nanomaterials for Mass Cytometry and Imaging: Advances and Biomedical Applications

Nano Biomedicine and Engineering
Mass cytometry (cytometry by time-of-flight (CyTOF)) and imaging mass cytometry (IMC) are transformative technologies that combine flow cytometry principles with time-of-flight mass spectrometry (TOF-MS).
Dongling Jia, Minhui Cui, Weiwei Wang, Jielin Sun, Xiaoxiang Chen   +4 more
doaj   +1 more source

Single-Cell Analysis of Refractory Celiac Disease Demonstrates Inter- and Intra-Patient Aberrant Cell HeterogeneitySummary

Cellular and Molecular Gastroenterology and Hepatology, 2022
Background & Aims: Refractory celiac disease type II (RCDII) is a rare indolent lymphoma in the small intestine characterized by a clonally expanded intraepithelial intracellular CD3+surfaceCD3-CD7+CD56- aberrant cell population.
Tessa Dieckman, Mette Schreurs, Ahmed Mahfouz, Yvonne Kooy-Winkelaar, Andra Neefjes-Borst, Gerd Bouma, Frits Koning   +6 more
doaj   +1 more source

Advances in mass cytometry and its applicability to digital pathology in clinical-translational cancer research

Advances in Laboratory Medicine, 2021
The development and subsequent adaptation of mass cytometry for the histological analysis of tissue sections has allowed the simultaneous spatial characterization of multiple components.
Cereceda Karina, Jorquera Roddy, Villarroel-Espíndola Franz   +2 more
doaj   +1 more source

β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1

Molecular Oncology, EarlyView.
Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero, Alejandro Belmonte‐Fernández, Mónica González‐Moreno, Laura Rodríguez‐Cordero, Begoña Pérez‐Valderrama, Joaquín Herrero‐Ruíz, Francisco Romero, Carmen Sáez, Miguel Á. Japón   +8 more
wiley   +1 more source

Radiosynthesis, in vitro and preliminary in vivo evaluation of the novel glutamine derived PET tracers [18F]fluorophenylglutamine and [18F]fluorobiphenylglutamine

, 2020
INTRODUCTION: Glucose has been deemed the driving force of tumor growth for decades. However, research has shown that several tumors metabolically shift towards glutaminolysis.
Baguet, Tristan, Beyzavi, M. Hassan, De Lombaerde, Stef, De Vos, Filip, Descamps, Benedicte, Donche, Sam, Goethals, Ingeborg, Hu, Jiyun, Lambe, Patricia, Pauwelyn, Glenn, Piron, Sarah, Vanhove, Christian, Verhoeven, Jeroen   +12 more
core   +1 more source

Optical excitation and detection of neuronal activity [PDF]

, 2017
Optogenetics has emerged as an exciting tool for manipulating neural activity, which in turn, can modulate behavior in live organisms. However, detecting the response to the optical stimulation requires electrophysiology with physical contact or ...
Gillette, Martha, Hu, Chenfei, Kim, Taewoo, Nastasa, Viorel, Popescu, Gabriel, Sam, Richard, Sengupta, Parijat, Shan, Mingguang, Wang, Minqi   +8 more
core   +2 more sources

A synthetic benzoxazine dimer derivative targets c‐Myc to inhibit colorectal cancer progression

Molecular Oncology, EarlyView.
Benzoxazine dimer derivatives bind to the bHLH‐LZ region of c‐Myc, disrupting c‐Myc/MAX complexes, which are evaluated from SAR analysis. This increases ubiquitination and reduces cellular c‐Myc. Impairing DNA repair mechanisms is shown through proteomic analysis.
Nicharat Sriratanasak, Bodee Nutho, Worawat Wattanathana, Narumon Phaonakrop, Bunnatut Panasawatwong, Katharina Erlenbach‐Wuensch, Sittiruk Roytrakul, Regine Schneider‐Stock, Pithi Chanvorachote   +8 more
wiley   +1 more source

A Versatile, Portable Intravital Microscopy Platform for Studying Beta-cell Biology In Vivo [PDF]

, 2019
The pancreatic islet is a complex micro-organ containing numerous cell types, including endocrine, immune, and endothelial cells. The communication of these systems is lost upon isolation of the islets, and therefore the pathogenesis of diabetes can only
Conteh, Abass M., Day, Richard N., Dunn, Kenneth W., Kamocka, Malgorzata M., Linnemann, Amelia K., Martinez, Michelle M., Mirmira, Raghavendra G., Orr, Kara S., Piñeros, Annie R., Reissaus, Christopher A., Tersey, Sarah A., Twigg, Ashley N.   +11 more
core   +1 more source