Results 71 to 80 of about 375,242 (294)

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Effect of acute immobilization on the hematological, red-ox and rheological parameters of erythrocytes in rats [PDF]

Al Ameen Journal of Medical Sciences, 2013
Objectives: Immobilization effects on antioxidant enzymes activities, red-ox variables and rheological parameters of erythrocytes were studied. Background: It is not clear to which extent immobilization-induced oxidative stress may affect erythrocyte ...
Artemy V. Novozhilov, Tat’yana V. Tavrovskaya, Michael I. Kalinski, Lev N. Katyukhin, Vadim A. Ivanov, Vladimir I. Morozov   +5 more
doaj  

A protective role of resveratrol against the effects of immobilization stress in corpora lutea of mice in early pregnancy

Journal of Integrative Agriculture, 2020
In the present study, we aimed to investigate a protective role for resveratrol against the effects of immobilization stress on corpora lutea (CL) of mice in early pregnancy.
Saif ULLAH, Sheeraz MUSTAFA, Wael ENNAB, Muhammad JAN, Muhammad SHAFIQ, Ngekure M.X. KAVITA, Zeng-peng LÜ, Da-gan MAO, Fang-xiong SHI   +8 more
doaj   +1 more source

Change in blood glucose level in rats after immobilization [PDF]

Experiments were carried out on male white rats divided into four groups. In group one the blood glucose level was determined immediately after immobilization.
Baskakova, G. M., Chepurnov, S. A., Platonov, R. D.   +2 more
core   +1 more source

Multivalent glycan arrays [PDF]

, 2019
Glycan microarrays have become a powerful technology to study biological processes, such as cell–cell interaction, inflammation, and infections. Yet, several challenges, especially in multivalent display, remain.
Bordoni, Vittorio, Delbianco, Martina, Loeffler, Felix F., Mende, Marco, Seeberger, Peter H., Tsouka, Alexandra   +5 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Effects of immobilization on spermiogenesis [PDF]

The influence of immobilization stress on spermiogenesis in rats was investigated. After 96 hour immobilization, histological changes began to manifest themselves in the form of practically complete disappearance of cell population of the wall of ...
Meitner, E. R.
core   +1 more source

UHMWPE/SBA-15 nanocomposites synthesized by in situ polymerization [PDF]

, 2016
Different nanocomposites have been attained by in situ polymerization based on ultra-high molecular weight polyethylene (UHMWPE) and mesoporous SBA-15, this silica being used for immobilization of the FI catalyst bis [N-(3-tert-butylsalicylidene)-2,3,4,
Adewole, Amoli, Ana E. Ferreira, Baier, Bakshi, Beck, Beloshenko, Bento, Bryliakov, Campos, Campos, Campos, Carlini, Cerrada, Cerrada, Chen, Cui, Cybo, Dangsheng, Deng, Dong, Dorigato, Díez-Pascual, Ernesto Pérez, Guo, Henri Cramail, Ianuzzi, Ivanchev, Ivanchev, Iwashita, João P. Lourenço, Kaminsky, Katti, Klimova, Kresge, Kurek, Kurtz, M. Rosário Ribeiro, Makio, Makio, Maria L. Cerrada, Mitani, Oliver, Olmos, Panchenko, Park, Quinn, Ribeiro, Sandaroos, Severn, Sinfrônio, Spiegelberg, Sánchez, Talebi, Tjong, Vicente Lorenzo, Wypych, Xu, Zhang, Zhao, Zohuri   +60 more
core   +3 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

A novel interdigitated capacitor based biosensor for detection of cardiovascular risk marker [PDF]

, 2009
C-reactive protein (CRP) is a potential biomarker whose elevated levels in humans determine cardiovascular disease risk and inflammation. In this study, we have developed a novel capacitive biosensor for detection of CRP-antigen using capacitor with ...
Davidson, Jimmy L., Gürbüz, Yaşar, Kang, Weng P., Qureshi, Anjum   +3 more
core   +1 more source