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Immunofluorescence Staining [PDF]
Current Protocols in Cell Biology, 1998 AbstractThis unit provides a protocol for indirect immunofluorescence, which is a method that provides information about the locations of specific molecules and the structure of the cell. Antibody molecules for a specific target molecule are exposed to the cell or tissue being investigated.
J. Donaldson
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Role of direct immunofluorescence in dermatological disorders
Indian Dermatology Online Journal, 2015 Background: Direct immunofluorescence (DIF) test for tissue-bound autoantibodies, has been found to be of value in the diagnosis of several dermatological disorders.
V. Mysorekar +2 more
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Nature Cancer, 2023 Precision medicine is critically dependent on better methods for diagnosing and staging disease and predicting drug response. Histopathology using hematoxylin and eosin (H&E)-stained tissue (not genomics) remains the primary diagnostic method in cancer ...
Jia-Ren Lin +13 more
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Primary Leiomyosarcoma of the Glans
Frontiers in Oncology, 2022 Penile leiomyosarcoma isz an extremely uncommon entity that rarely occurs in the glans. Due to the limited number of cases described in literature, guidelines regarding non-surgical treatment, prognosis, and management remain equivocal.
Raimundo Nonato Gois da Costa Junior +13 more
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Fully automated sequential immunofluorescence (seqIF) for hyperplex spatial proteomics
bioRxiv, 2023 Tissues are complex environments where different cell types are in constant interaction with each other and with non-cellular components. Preserving the spatial context during proteomics analyses of tissue samples has become an important objective for ...
F. Rivest +21 more
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Non-Coding RNA in Penile Cancer
Frontiers in Oncology, 2022 Penile cancer (PC) still presents a health threat for developing countries, in particular Brazil. Despite this, little progress has been made on the study of markers, including molecular ones, that can aid in the correct management of the patient ...
Jaqueline Diniz Pinho +13 more
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Multiplex Immunofluorescence: A Powerful Tool in Cancer Immunotherapy
International Journal of Molecular Sciences, 2023 Traditional immunohistochemistry (IHC) has already become an essential method of diagnosis and therapy in cancer management. However, this antibody-based technique is limited to detecting a single marker per tissue section.
W. Sheng +7 more
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BMC Cancer, 2022 Background Although penile cancer (PC) is uncommon in developed countries, it is widespread in developing countries. The state of Maranhão (Northeast, Brazil) has the highest global incidence recorded for PC, and, despite its socioeconomic vulnerability,
Antonio Augusto Lima Teixeira Júnior +20 more
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Frequency of C4d Positivity in Membranous and other Glomerulonephritis in Renal Biopsy Specimens in a Tertiary Care Hospital [PDF]
JLUMHS, 2022 OBJECTIVE: To determine the frequency of C4d positivity in Membranous and other Glomerulonephritis in renal biopsy specimens. METHODOLOGY: This cross-sectional study was carried out in the Pathology Department, Ziauddin Medical University and Hospital ...
Maria Shafique +4 more
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IMMUNOFLUORESCENCE COUNTERSTAINS [PDF]
Journal of Histochemistry & Cytochemistry, 1974 Counterstains in the direct and indirect immunofluorescence methods may be used to reduce or eliminate background nonspecific staining or autofluorescence and to increase cellular and tissue detail. The advantages and disadvantages of a number of such counterstains in terms of (a) elimination of background fluorescence, (b) blocking of specific ...
E A, Schenk, C J, Churukian
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