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Immunofluorescence Staining [PDF]
AbstractThis unit provides a protocol for indirect immunofluorescence, which is a method that provides information about the locations of specific molecules and the structure of the cell. Antibody molecules for a specific target molecule are exposed to the cell or tissue being investigated.
openaire +4 more sources
Background Oculosporidiosis (ocular rhinosporidiosis) accounts for 15% of cases of rhinosporidiosis, which is a chronic granulomatous disease and is endemic in India and Sri Lanka.
Francílio Araújo Almeida +4 more
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Tomato zonate spot virus (TZSV) is a phytopathogen of the genus Orthotospovirus (Bunyaviridae) that is widespread in many areas of Southwest China. TZSV is mainly transmitted by Frankliniella occidentalis, but its exact infection route remains unclear ...
Yong Chen +13 more
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Primary ciliary dyskinesia (PCD) is a rare genetic disease characterized by dyskinetic cilia. Respiratory symptoms usually start at birth. The lack of diagnostic gold standard tests is challenging, as PCD diagnostics requires different methods with high ...
Loretta Müller +17 more
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Expression of Foxp3 in colorectal cancer but not in Treg cells correlates with disease progression in patients with colorectal cancer [PDF]
Background: Regulatory T cells (Treg) expressing the transcription factor forkhead-box protein P3 (Foxp3) have been identified to counteract anti-tumor immune responses during tumor progression. Besides, Foxp3 presentation by cancer cells itself may also
A Ho-Pun-Cheung +45 more
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The small GTPase Rab29 is a common regulator of immune synapse assembly and ciliogenesis [PDF]
Acknowledgements We wish to thank Jorge Galán, Gregory Pazour, Derek Toomre, Giuliano Callaini, Joel Rosenbaum, Alessandra Boletta and Francesco Blasi for generously providing reagents and for productive discussions, and Sonia Grassini for technical ...
A Batista +51 more
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Immunocytochemical localization of chromatin regions UV-microirradiated in S phase or anaphase : Evidence for a territorial organization of chromosomes during cell cycle of cultured Chinese hamster cells [PDF]
Chinese hamster cells (M3-1 line) in S phase were laser-UV-microirradiated (λ, 257 nm) at a small site of the nucleus. Cells were fixed either immediately thereafter or in subsequent stages of the cell cycle, including prophase and metaphase.
Avivi +33 more
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The aim is to study the features of the formation of neutrophil extracellular traps in the blood and gut-associated lymphoid tissue with salmonella-induced inflammation against the background of the administration of vancomycin and B.
Yu. V. Bukina +5 more
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MRE11 facilitates the removal of human topoisomerase II complexes from genomic DNA [PDF]
Topoisomerase II creates a double-strand break intermediate with topoisomerase covalently coupled to the DNA via a 5'-phosphotyrosyl bond. These intermediate complexes can become cytotoxic protein-DNA adducts and DSB repair at these lesions requires ...
Austin, Caroline +10 more
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Although the concept of combining immunofluorescence (IF) with single-molecule RNA fluorescence in situ hybridization (smRNA FISH) seems obvious, the specific materials used during IF and smRNA FISH make it difficult to perform these procedures ...
Jakub Kochan, Mateusz Wawro, Aneta Kasza
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