Results 21 to 30 of about 1,052,494 (379)

Brain-reactive autoantibodies in neuropsychiatric systemic lupus erythematosus

Frontiers in Immunology, 2023
IntroductionThe pathogenesis of neuropsychiatric systemic lupus erythematosus (NPSLE) is widely unknown, and the role of autoantibodies is still undetermined.MethodsTo identify brain-reactive autoantibodies possibly related to NPSLE, immunofluorescence ...
Cristina Cocco, Elias Manca, Giulia Corda, Maria Maddalena Angioni, Maria Maddalena Angioni, Barbara Noli, Mattia Congia, Francesco Loy, Michela Isola, Elisabetta Chessa, Alberto Floris, Alberto Floris, Lorena Lorefice, Luca Saba, Luca Saba, Alessandro Mathieu, Gian Luca Ferri, Alberto Cauli, Alberto Cauli, Matteo Piga, Matteo Piga   +20 more
doaj   +1 more source

Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence

International Journal of Molecular Sciences, 2022
Immunostaining has emerged as one of the most common and valuable techniques that allow the localization of proteins at a quantitative level within cells and tissues using antibodies coupled to enzymes, fluorochromes, or colloidal nanogold particles. The
Ricardo Piña, Alma I. Santos-Díaz, Erika Orta-Salazar, Azucena Ruth Aguilar-Vazquez, Carola A. Mantellero, Isabel Acosta-Galeana, Argel Estrada-Mondragón, Mara Prior-Gonzalez, Jadir Isai Martinez-Cruz, Abraham Rosas-Arellano   +9 more
semanticscholar   +1 more source

Overview of multiplex immunohistochemistry/immunofluorescence techniques in the era of cancer immunotherapy

Cancer Communications, 2020
Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of limitations, including high inter‐observer variability and the capacity to label only one marker per
W. Tan, S. N. Nerurkar, H. Cai, Harry Ho Man Ng, Duo-qing Wu, Yu Ting Felicia Wee, J. Lim, J. Yeong, T. Lim   +8 more
semanticscholar   +1 more source

Targeting PKCθ promotes satellite cell self-renewal [PDF]

, 2020
Skeletal muscle regeneration following injury depends on the ability of satellite cells (SCs) to proliferate, self-renew, and eventually differentiate. The factors that regulate the process of self-renewal are poorly understood. In this study we examined
Benedetti, Anna, Bouché, Marina, Fiore, Piera Filomena, Lozanoska-Ochser, Biliana, Madaro, Luca   +4 more
core   +1 more source

Multi-institutional TSA-amplified Multiplexed Immunofluorescence Reproducibility Evaluation (MITRE) Study

Journal for ImmunoTherapy of Cancer, 2021
Background Emerging data suggest predictive biomarkers based on the spatial arrangement of cells or coexpression patterns in tissue sections will play an important role in precision immuno-oncology.
J. Taube, K. Roman, Elizabeth L Engle, Chichung Wang, C. Ballesteros-Merino, S. Jensen, J. McGuire, Mei Jiang, Carla Coltharp, B. Remeniuk, I. Wistuba, D. Locke, E. Parra, B. Fox, D. Rimm, C. Hoyt   +15 more
semanticscholar   +1 more source

Royal Jelly Suppresses Invasive Potential of Colorectal Cancer Cells by Attenuating Vimentin and Snail

Engineering Proceedings, 2023
Royal jelly (RJ), as an exclusive bee product, showed beneficial effects on various human pathological conditions, including cancer. Therefore, we examined the effects of RJ on the suppression of invasiveness of SW-480 cells and protein expression of ...
Milena Jovanović, Katarina Virijević, Dejan Arsenijević, Katarina Pecić, Dragana Šeklić   +4 more
doaj   +1 more source

A cohort study among 402 patients with penile cancer in Maranhão, Northeast Brazil with the highest worldwide incidence

BMC Research Notes, 2020
Objective Maranhão State—Northeast Brazil–has the world’s highest incidence of penile cancer. This study describes the epidemiological, histopathological and clinical profile of patients stricken across that Brazilian state.
Ciro Bezerra Vieira, Antonio Teixeira-Júnior, Laisson Feitoza, Jaqueline Pinho, José Calixto, Francisco Sérgio Moura Silva do Nascimento, Marcos Adriano Garcia Campos, Joyce Lages, Antonio Machado Alencar Junior, Fernando Soares, Isabela Cunha, Gyl Eanes Barros Silva   +11 more
doaj   +1 more source

Single-cell detection of primary transcripts, their genomic loci and nuclear factors by 3D immuno-RNA/DNA FISH in T cells

Frontiers in Immunology, 2023
Over the past decades, it has become increasingly clear that higher order chromatin folding and organization within the nucleus is involved in the regulation of genome activity and serves as an additional epigenetic mechanism that modulates cellular ...
Eralda Salataj, Eralda Salataj, Charalampos G. Spilianakis, Charalampos G. Spilianakis, Julie Chaumeil   +4 more
doaj   +1 more source

The Society for Immunotherapy in Cancer statement on best practices for multiplex immunohistochemistry (IHC) and immunofluorescence (IF) staining and validation

Journal for ImmunoTherapy of Cancer, 2020
Objectives The interaction between the immune system and tumor cells is an important feature for the prognosis and treatment of cancer. Multiplex immunohistochemistry (mIHC) and multiplex immunofluorescence (mIF) analyses are emerging technologies that ...
J. Taube, Guray Akturk, Michael Angelo, Elizabeth L Engle, S. Gnjatic, Shirley Greenbaum, N. Greenwald, C. Hedvat, T. Hollmann, J. Juco, E. Parra, M. Rebelatto, D. Rimm, J. Rodriguez-Canales, K. Schalper, E. Stack, Cláudia S Ferreira, Konstanty Korski, Ana Lako, S. Rodig, Emanuel Schenck, K. Steele, M. Surace, M. Tetzlaff, Katharina von Loga, I. Wistuba, C. Bifulco   +26 more
semanticscholar   +1 more source

Immunocytochemical localization of chromatin regions UV-microirradiated in S phase or anaphase : Evidence for a territorial organization of chromosomes during cell cycle of cultured Chinese hamster cells [PDF]

, 1983
Chinese hamster cells (M3-1 line) in S phase were laser-UV-microirradiated (λ, 257 nm) at a small site of the nucleus. Cells were fixed either immediately thereafter or in subsequent stages of the cell cycle, including prophase and metaphase.
Avivi, Bennett, Berns, Boveri, Boveri, Comings, Cornelis, Cornelis, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Cremer, Earnshaw, Gray, Hager, Hancock, Hancock, Hens, Laemmli, Rabl, Regan, Sakharov, Sedat, Sperling, Stack, Trosko, Vogel, Zorn   +33 more
core   +1 more source