Results 1 to 10 of about 49,892 (224)

Bacterial Proteins and Their Proposed Interactions with Fc or Fab Fragments of Immunoglobulins

Biochemistry & Physiology: Open Access, 2014
The reactivity of Immunoglobulin Binding Proteins (IBP) to Fc and/or Fab fragments of immunoglobulins was summarized in this review. Staphylococcal protein A (SpA), Streptococcal protein G and Peptostreptococcal protein L (SpL) were the IBP reported. SpA reacted with IgG from skunk, coyote, raccoon, mule and donkey.
Sehlule Vuma, Wayne Mohammed, Angel A. Justiz Vaillant   +2 more
semanticscholar   +4 more sources

Detection of immunoglobulin A1 protease-induced Fab alpha fragments on dental plaque bacteria [PDF]

Infection and Immunity, 1991
The mechanisms by which immunoglobulin A1 (IgA1) protease activity may enable bacteria to evade the effect of specific secretory IgA (S-IgA) antibodies are not clear. A possibility which has received indirect experimental support is that bacteria, as a consequence of the protease activity, become coated with incompetent Fab alpha fragments instead of ...
T Ahl, J Reinholdt
semanticscholar   +5 more sources

Cell-free synthesis of functional and endotoxin-free antibody Fab fragments by translocation into microsomes

BioTechniques, 2012
A eukaryotic cell-free system based on Spodoptera frugiperda cells was developed for the convenient synthesis of Fab antibody fragments and other disulfide bridge containing proteins.
Helmut Merk, Christine Gless, Barbara Maertens, Michael Gerrits, Wolfgang Stiege   +4 more
doaj   +2 more sources

Allosteric inhibition of IgE–FcεRI interactions by simultaneous targeting of IgE F(ab’)2 epitopes [PDF]

Communications Biology
Immunoglobulin E (IgE) plays pivotal roles in allergic diseases through interaction with a high-affinity receptor (FcεRI). We established that Fab fragments of anti-IgE antibodies (HMK-12 Fab) rapidly dissociate preformed IgE-FcεRI complexes in a ...
Takao Hirano, Akemi Koyanagi, Hideo Ago, Masaki Yamamoto, Jiro Kitaura, Masataka Kasai, Ko Okumura   +6 more
doaj   +2 more sources

Discovery and Characterization of Unusual O-Linked Glycosylation of IgG4 Antibody Using LC-MS. [PDF]

Rapid Commun Mass Spectrom
ABSTRACT Background Consensus is that immunoglobulin IgG4 contains only N‐linked glycosylation. The analysis of several batches of commercial biopharmaceutical product Dupixent using top‐down intact mass spectrometry revealed that this IgG4 features a small amount of O‐linked glycosylation in the Fab region.
Janecki DJ, Kao-Scharf CY, Hoffmann A.
europepmc   +2 more sources

Small-angle X-ray scattering of engineered antigen-binding fragments: the case of glycosylated Fab from the Mannitou IgM antibody. [PDF]

Acta Crystallogr F Struct Biol Commun
This study provides a biochemical and biophysical analysis of Mannitou Fab, highlighting the intricate balance between stability, flexibility and function in engineered antibody fragments. The results underscore the critical role of glycosylation in modulating the oligomerization behaviour of Fab and preventing inappropriate intermolecular interactions,
Semwal S, Karamolegkou M, Flament S, Raouraoua N, Verstraete K, Thureau A, Wien F, Bray F, Savvides SN, Bouckaert J.   +9 more
europepmc   +2 more sources

A Case Report of Crotalidae Immune F(ab’)2-associated Coagulopathy Recurrence in a Preschool-age Child [PDF]

Clinical Practice and Cases in Emergency Medicine
Introduction: Pit viper envenomation may cause coagulopathy. The coagulopathy has been treated with crotalidae polyvalent immune fragment antigen-binding (Fab) ovine antivenom for the last few decades in the United States and usually corrects the acute ...
Jean C.Y. Lo, E. Lea Walters, Brian Wolk
doaj   +2 more sources

Near‐Infrared Optical Sensing of Biomacromolecules with Upconversion Nanoplatforms

Advanced Photonics Research, Volume 4, Issue 1, January 2023., 2023
In the past decades, continuous efforts have been dedicated to develop intelligent upconversion nanoplatforms (UCNs) for optical sensing of diverse biomacromolecules including DNA, RNA, proteins, and enzymes. Optical sensing and imaging have perceived massive success in biomedical analysis and disease diagnosis in terms of minimal invasiveness, good ...
Liben Yan, Zhimin Wang
wiley   +1 more source

Strategies for the production of isotopically labelled Fab fragments of therapeutic antibodies in Komagataella phaffii (Pichia pastoris) and Escherichia coli for NMR studies

PLoS ONE, 2023
The importance and fast growth of therapeutic monoclonal antibodies, both innovator and biosimilar products, have triggered the need for the development of characterization methods at high resolution such as nuclear magnetic resonance (NMR) spectroscopy.
Donald Gagné, Muzaddid Sarker, Geneviève Gingras, Derek J Hodgson, Grant Frahm, Marybeth Creskey, B. Lorbetskie, Stewart Bigelow, Jun Wang, Xu Zhang, Michael J W Johnston, Huixin Lu, Yves Aubin   +12 more
semanticscholar   +1 more source

Effect of IgG fragments in IVIG(pH4)on phagocytosis of sensitized erythrocytes by macrophage

Zhongguo shuxue zazhi, 2022
Objective To research the effect of the Fc, Fab and F(ab′)2 fragments of immunoglobulin G, the main components of Human Immunoglobulin(pH4) for Intravenous Injection(IVIG), on the phagocytic function of macrophages derived from THP-1 cells. Methods First
Liyuan ZHU, Wei ZHANG, Mingxia HOU, Qin LIU, Yuyan QIN, Li Ma, Changqing LI   +6 more
doaj   +1 more source