Results 161 to 170 of about 119,597,839 (255)

Engineered Transformer Base Editor with Enhanced Editing Efficiency

Advanced Science, EarlyView.
A highly efficient transformer base editor system achieves robust genomic editing in a humanized mouse model. This work establishes a versatile and translatable platform, opening new avenues for precision gene therapy. ABSTRACT Canonical cytosine base editors (CBEs) achieve precise C‐to‐T conversions without inducing DNA double‐strand breaks (DSBs ...
Bowen Chen, Letong Liang, Rui Xu, Tianyi Wang, Hongyan Chen, Daru Lu   +5 more
wiley   +1 more source

In Silico Design and Computational Characterization of Novel Chimeric Multiepitope Antigens for Mpox Serosurveillance: An Immunoinformatics Approach. [PDF]

Health Sci Rep
Akwelle RN, Shey RA, Efeti MT, Acha LN, Nchanji GT, Tangan YAS, Chick JA, Yaah NE, Shinyuy LM, Shintouo CM, Yengo BN, Ayuk AB, Engarimbi JE, Gwei KY, Tebo BT, Nebangwa DN, Tamnjong BM, Vanhamme L, Souopgui J, Titanji VPK, Ghogomu SM.   +20 more
europepmc   +1 more source

Reversible, Chemically Gated FRET via Ligand‐Activated Acceptors

Advanced Science, EarlyView.
Fluorogen binding turns the compact FAST tag into a tunable FRET acceptor, with ligand chemistry programming spectral overlap and excited‐state lifetimes. Fluorescence‐lifetime imaging reads out energy transfer through the shortened donor lifetime, while simply adding or washing out the dye switches FRET on and off, reversibly mapping the supramodular ...
Nivedita Singh, Smruti Ranjan Nayak, Sanjay Kumar Mohanty, Aishwarya Hanumantharaju, Amala Shaju, Mini Jose, Aravind Penmatsa, Deepak Nair   +7 more
wiley   +1 more source

In silico design and evaluation of a candidate multiepitope Salmonella vaccine construct targeting broad-spectrum protection in poultry. [PDF]

BMC Genomics
Bradshaw DJ, Monson MS, Bearson BL, Bearson SMD.   +3 more
europepmc   +1 more source

Design of a Novel Diamine Biosensor to Guide the Engineering of Escherichia coli for Cadaverine Overproduction

Advanced Science, EarlyView.
A high‐throughput screening platform was established by integrating an in vivo cadaverine biosensor with a fluorescence‐activated cell sorting (FACS) system. Biosensor‐driven specific exporter tuning, pathway optimization and genome‐wide target mining were conducted to maximize cadaverine production in Escherichia coli.
Jia Feng, Getao Liu, Ding Ma, Jiaxiang Yuan, Xinyi Zhang, Weihao Wang, Chen Ma, Yang Liao, Siyuan Gao, Lei Qin, Jiao Feng, Sheng Xu, Xin Wang, Kequan Chen   +13 more
wiley   +1 more source

In silico design of a multiepitope vaccine against antibiotic drug-resistant Acinetobacter baumannii. [PDF]

Sci Rep
Khadka S, Khan MA, Iqbal S, Ullah A, Ahmad A, Khan NZ, Khattak A.   +6 more
europepmc   +1 more source

A three-dimensional and multivariate statistical analysis of serine-hydrolases active sites.

, 2012
EBERT, CYNTHIA, FOSCATO, MARCO, GARDOSSI, Lucia, FERRARIO, VALERIO   +3 more
core  

Spatial Transcriptomics Reveals a T Cell‐Mediated Microglial Activation Axis of Immune Checkpoint Inhibition‐Related Adverse Events in the Brain

Advanced Science, EarlyView.
Immune checkpoint inhibition (ICI) reshapes the hippocampal neuroimmune niche. Spatial transcriptomic profiling and human brain histological analyses identify neuroinflammation and altered neuronal and glial programs. Notably, T cell depletion prevents ICI‐driven microglial activation, establishing the T cell–microglia crosstalk axis as a driving ...
Devyani Swami, Sanad M. El‐Khatib, Suhas Sureshchandra, Elise Nguyen, Janaki Manoja Vinnakota, Robert Zeiser, Shivashankar Othy, Munjal M. Acharya   +7 more
wiley   +1 more source

3D analysis of catalytic differences of hydrolases by integrating modelling and mutivariate statistics

, 2012
EBERT, CYNTHIA, FOSCATO, MARCO, GARDOSSI, Lucia, FERRARIO, VALERIO, Svendsen, A., KNAPIC, Lorena, Besenmatter, W.   +6 more
core  

Matrix Rigidity Mechanoprimes Microglia for Inflammation Through Cytoskeletal‐to‐Nuclear Signaling and 3D Spatio‐Epigenomic Remodeling

Advanced Science, EarlyView.
Pathological tissue rigidity mechanoprimes microglia by enhancing actin cytoskeleton–nucleus coupling and chromatin opening at rigidity‐responsive cis‐regulatory elements (mechanoCREs). Subsequent NF‐κB/p65 signaling converges on this permissive regulatory state to amplify inflammatory gene expression and microglial activation.
Yu Xuan Meng, Jaegeon Joo, Yu Meng Li, Cheng Ji Li, Ali Taghizadeh, Seung Jae Shin, Buuvee Bayarkhangai, Tae‐Woo Ha, Sak Lee, Jaewoo Chung, Bo‐Eun Yoon, Hye Sung Kim, Inkyung Jung, Jung‐Hwan Lee, Kam W. Leong, Hae‐Won Kim   +15 more
wiley   +1 more source