Results 71 to 80 of about 32,158 (205)

Isolation Defines Identity: Functional Consequences of Extracellular Vesicle Purification Strategies

Advanced Healthcare Materials, EarlyView.
Four extracellular vesicle purification strategies are compared using ovarian‐cancer ascites and ES‐2 cell supernatants. A novel workflow links purification to function by combining particle‐normalized proteomics with matched cell‐free and cell‐based assays.
Christian Preußer, Dolores J. Salander, Witold Szymanski, Johannes Graumann, Yukai Wang, Kai Zhao, Jörg W. Bartsch, María Gómez‐Serrano, Daniel Bachurski, Silke Reinartz, Elke von Pogge von Strandmann   +10 more
wiley   +1 more source

Comprehensive evaluation of enzymatic proteolysis for amlyoid reduction in macular amyloidosis by in vitro, in vivo and in silico methods

IP Indian Journal of Clinical and Experimental Dermatology
Skin diseases represent a significant burden on global healthcare systems, with protein deposition playing a pivotal role in their pathogenesis, including conditions such as macular amyloidosis. This study aimed to assess the proteolytic activity of the enzyme Papain on deposited protein, offering insights into potential therapeutic strategies.
Vrushali Neve, Vrushali Bhalchim, Pawan Nandkishor Karwa, Ajay Sabu, Swapnil Runje   +4 more
openaire   +1 more source

BRUCE: a program for the detection of transfer-messenger RNA genes in nucleotide sequences [PDF]

, 2002
A computer program, BRUCE, was developed for the identification of transfer‐messenger RNA (tmRNA) genes. The program employs heuristic algorithms to search for a tRNAAla‐like secondary structure surrounding a short sequence encoding the tag peptide.
Laslett, D., Canback, B., Andersson, S.
core   +1 more source

Simulating Brain Tumor Heterogeneity with a Multiscale Agent-Based Model: Linking Molecular Signatures, Phenotypes and Expansion Rate

, 2007
We have extended our previously developed 3D multi-scale agent-based brain tumor model to simulate cancer heterogeneity and to analyze its impact across the scales of interest.
Deisboeck, Thomas S., Strouthos, Costas G., Wang, Zhihui, Zhang, Le   +3 more
core   +1 more source

B4GALNT4‐Mediated Glycosylation of PDK1 Activates the PI3K‐AKT Signaling Pathway to Promote Prostate Cancer Progression

Advanced Science, EarlyView.
This study identifies B4GALNT4 as a key driver of prostate cancer (PCa). It shows that B4GALNT4 glycosylates PDK1 protein at the N531 site, which stabilizes the PDK1 and constitutively activates the PI3K‐AKT pathway. This mechanism promotes tumor cell proliferation, migration, and invasion. The findings establish the B4GALNT4‐PDK1 glycosylation axis as
Shaoqin Jiang, Mengqiang Li, Qingfu Su, Ruiling Dou, Shaoshan Lin, Jili Zhang, Xiaochen Sun, Haolan Yu, Wei Bao, Min Qu, Yan Wang, Chenghua Yang, Xu Gao   +12 more
wiley   +1 more source

AIP1 is a novel Agenet/Tudor domain protein from Arabidopsis that interacts with regulators of DNA replication, transcription and chromatin remodeling [PDF]

, 2015
Background: DNA replication and transcription are dynamic processes regulating plant development that are dependent on the chromatin accessibility. Proteins belonging to the Agenet/Tudor domain family are known as histone modification "readers" and ...
Barroso-Neto, Ito Liberato, Brasil, Juliana Nogueira, Cabral, Luiz Mors, Eloy, Nubia Barbosa, Ferreira, Paulo CG, Gonzalez Sanchez, Nathalie, Hemerly, Adriana S, Inzé, Dirk, Perdigão Grangeiro, Leticia P, Primo, Luiza MF   +9 more
core   +3 more sources

PAF Triggered Pyroptotic NETosis Aggravates Myocardial Ischemia/Reperfusion Injury

Advanced Science, EarlyView.
Platelet activating factor (PAF) secreted by cardiomyocytes during MI/R, drives NETs formation and subsequent NETosis. PAF/NETosis signaling activation is an initiative and causal factor in driving MI/R injury. This study identifies dapagliflozin as a potent NETosis inhibitor, and demonstrates that manipulating PAF‐NETosis signal by dapagliflozin or ...
Jiawei Wu, Shule Zhang, Ruofan Du, Lina Kang, Guodong Zhao, Xue Bao, Haochi Yang, Ziqing Xie, Tianyu He, Huiyong Sun, Haiping Hao, Lijuan Cao   +11 more
wiley   +1 more source

A label free chemoproteomic-based platform to disclose cannabidiol molecular mechanism of action on chronic myelogenous leukemia cancer cells

Heliyon
The discovery of the interactome of cannabidiol (CBD), a non-psychoactive cannabinoid from Cannabis sativa L., has been here performed on chronic myelogenous leukemia cancer cells, using an optimized chemo-proteomic stage, which links Drug Affinity ...
Sara Ceccacci, Lorenzo Corsi, Lucio Spinelli, Clarissa Caroli, Matilde Marani, Lisa Anceschi, Matteo Mozzicafreddo, Federica Pellati, Maria Chiara Monti   +8 more
doaj   +1 more source

Ligand binding to an Allergenic Lipid Transfer Protein Enhances Conformational Flexibility resulting in an Increase in Susceptibility to Gastroduodenal Proteolysis [PDF]

, 2016
Non-specific lipid transfer proteins (LTPs) are a family of lipid-binding molecules that are widely distributed across flowering plant species, many of which have been identified as allergens.
A Bossios, A Palacin, A Palacin, A Petersen, AE Flinterman, AJ de Jong, B Bakan, B Bakan, B Berecz, CD Kane, D Charvolin, D Sy, DJ Cooper, E Gincel, E Vassilopoulou, EA Pastorello, EA Pastorello, EN Mills, EN Mills, F Sievers, FJ Moreno, FJ Moreno, G De Libero, H Breiteneder, JC Kader, JC Phillips, JP Douliez, K Chae, K Hoffmann-Sommergruber, K Lindorff-Larsen, K Vanommeslaeghe, KC Verhoeckx, L Lei, L Mirotti, LA Kelley, M Bublin, M Fernandez-Rivas, M Minekus, M Strohalm, M Subirade, MH Lerche, MM Hofliger, N Pasquato, P Gouet, P Sodano, R Asero, R Izzo, R Sanchez-Monge, R Wijesinha-Bettoni, R Wijesinha-Bettoni, S Bengmark, S Gaier, S Jegou, S Jo, S Tassin-Moindrot, S Yapoudjian, SJ Hubbard, SU Nielsen, TH Yeats, W Humphrey, WR Morrison, Y Cheng   +61 more
core   +1 more source

ZBTB21 Is a Dual Suppressor of Pyroptosis and MHC‐I Antigen Presentation That Promotes Tumor Immune Evasion

Advanced Science, EarlyView.
ZBTB21 is a transcription factor that epigenetically suppresses pyroptosis and MHC‐I antigen presentation, enabling tumor immune evasion. Genetic ablation of ZBTB21 activates pyroptotic cell death and enhances antigen presentation, recruiting CD8+ T cells to overcome immune checkpoint blockade resistance.
Lei Zhao, Linlin Sheng, Jianhao Qiu, Junjie Ma, Kai Jin, Binggong Zhao, Tianrun Miao, Jing Chen, Yehong Li, Zhan Zhang, Dongfeng Sun, Yongmeng Li, Hui Tian   +12 more
wiley   +1 more source