Results 81 to 90 of about 112,390 (279)

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Ratiometric control for differentiation of cell populations endowed with synthetic toggle switches

, 2019
We consider the problem of regulating by means of external control inputs the ratio of two cell populations. Specifically, we assume that these two cellular populations are composed of cells belonging to the same strain which embeds some bistable memory ...
di Bernardo, Mario, Fiore, Davide, Salzano, Davide   +2 more
core   +1 more source

In silico assessment of biomedical products: the conundrum of rare but not so rare events in two case studies [PDF]

, 2017
In silico clinical trials, defined as “The use of individualized computer simulation in the development or regulatory evaluation of a medicinal product, medical device, or medical intervention,” have been proposed as a possible strategy to reduce the ...
Adam Himes, American Diabetes Association, Anderson SM, Andreassen S, Bassingthwaighte JB, Bassingthwaighte JB, Basu R, Baxter W, Benson N, Bode BW, Boris Kovatchev, Bosi GM, Bosmans L, Britten RD, Campbell MP, Carriero A, Chen L, Christie GR, Claudio Cobelli, Cobelli C, Cobelli C, Cooling MT, Cristofolini L, Dalla Man C, Danaei G, DiMasi JA, El Makssoud H, Falcinelli C, Fernandez JW, Fernandez JW, Fregly BJ, Fritsch A, Gasser TC, Ghanbari J, Haddad T, Haddad T, Hamed E, Heidlauf T, Hinshaw L, Hunter PJ, Kennergren C, Kohout J, Kovatchev BP, Kropff J, Krueger MW, Lavagnino M, Lehmann ED, Leone-Bay A, Li X, Li X, Lloyd CM, Lodola A, Lungu A, Maceri F, Mah JT, Marco Viceconti, Mark Palmer, Martelli S, Martelli S, Martelli S, Meagher JM, Morris PD, Nair KS, Nørgaard BL, Oberhofer K, Panteleon AE, Porzio S, Predoi-Racila M, Qasim M, Ravera EP, Renard E, Riccio AI, Salzsieder E, Scheys L, Scheys L, Shaw JE, Shim VB, Smith DW, Smith N, Sorensen JT, Srinivasan R, Steil GM, Swee JKY, Szczesny SE, Tarek Haddad, The Diabetes Control and Complications Trial Research Group, Toffanin C, UK Prospective Diabetes Study Group (UKPDS), Vettoretti M, Viceconti M, Viceconti M, Visentin R, Visentin R, Visentin R, Visentin R, Weis JA, Zarins CK, Zimmet P   +97 more
core   +1 more source

Complete populations of virtual patients forin silicoclinical trials

Bioinformatics, 2020
AbstractMotivationModel-based approaches to safety and efficacy assessment of pharmacological drugs, treatment strategies or medical devices (In Silico Clinical Trial, ISCT) aim to decrease time and cost for the needed experimentations, reduce animal and human testing, and enable precision medicine. Unfortunately, in presence of non-identifiable models
S Sinisi, V Alimguzhin, T Mancini, E Tronci, B Leeners   +4 more
openaire   +4 more sources

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

An extensive generative framework and multiphasic verification strategy for synthesising CT images of diverse anatomies: towards the utilisation for in silico clinical trials

Computer Methods in Biomechanics and Biomedical Engineering: Imaging & Visualization
The conventional approach of testing the safety and efficacy of medical devices/drugs is done through clinical trials. These trials are time-consuming, expensive and pose ethical issues.
Rajarajeswari Ganesan, Sina Amirrajab, Cian M. Scannell, Hester A. Gietema, Sjeng Quicken, Antonino A. La Mattina, Tim A. E. J. Boymans, Frans N. van de Vosse, Wouter Huberts   +8 more
doaj   +1 more source

Timing feedback-inhibition of the male reproductive hormone axis [PDF]

, 2008
Hormonal methods of male contraception have addressed feedback-inhbition of the cascade of hormones that has evolved to regulate sperm production but high concentrations of testosterone (T) in the blood have not worked satisfactorily.
Amit Misra, Gopal Gupta, K Subramanian Venkatesh, Ritu R. Malik, Shailesh S. Tondwal   +4 more
core   +2 more sources

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Exploring Protein-Protein Interactions as Drug Targets for Anti-cancer Therapy with In Silico Workflows [PDF]

, 2017
We describe a computational protocol to aid the design of small molecule and peptide drugs that target protein-protein interactions, particularly for anti-cancer therapy. To achieve this goal, we explore multiple strategies, including finding binding hot
A Goncearenco, A Goncearenco, A Marchler-Bauer, A Truszkowski, AA Bogan, B Graves, B Ma, BA Shoemaker, BA Shoemaker, BA Shoemaker, BJ Smith, CA Goble, CM Yates, D Petrey, E Cukuroglu, FP Davis, H Perez-Sanchez, HS Haase, J Bhagat, J Cinatl, JA Wells, K Wolstencroft, M Guharoy, M Li, M Li, M Li, M Petukh, M Tyagi, MK Gilson, MP Mazanetz, N Estrada-Ortiz, P Aloy, P Aloy, P Filippakopoulos, R Mosca, RR Thangudu, S Beisken, S Kim, S Shangary, S Teng, T Rolland, W Yang, WS Valdar, Y Wang, Y Zhao   +44 more
core   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

FEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao, Daiki Kobayashi, Shogo Hori, Osamu Saitoh   +3 more
wiley   +1 more source