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Chromogenic in situ hybridization technique for detecting porcine circovirus 3 in lung and lymphoid tissues [PDF]

open access: yesVeterinary World, 2023
Background and Aim: Porcine circovirus 3 (PCV3) was recently reported in Malaysian commercial pig population in 2020 by conventional polymerase chain reaction (PCR), revealing a molecular prevalence of 17.02% in the sampled domestic pig population.
Chew Yee Tan   +4 more
doaj   +1 more source

Hypothalamic Nesfatin-1 Resistance May Underlie the Development of Type 2 Diabetes Mellitus in Maternally Undernourished Non-obese Rats

open access: yesFrontiers in Neuroscience, 2022
Intrauterine growth retardation (IUGR) poses a high risk for developing late-onset, non-obese type 2 diabetes (T2DM). The exact mechanism underlying this phenomenon is unknown, although the contribution of the central nervous system is recognized.
Máté Durst   +7 more
doaj   +1 more source

Cytogenetics and Consequences of Polyploidization on Different Biotic-Abiotic Stress Tolerance and the Potential Mechanisms Involved

open access: yesPlants, 2022
The application of polyploidy in sustainable agriculture has already brought much appreciation among researchers. Polyploidy may occur naturally or can be induced in the laboratory using chemical or gaseous agents and results in complete chromosome ...
Md Mazharul Islam   +6 more
doaj   +1 more source

Imaging biofilms using fluorescence in situ hybridization: seeing is believing

open access: yesFrontiers in Cellular and Infection Microbiology, 2023
Biofilms are complex structures with an intricate relationship between the resident microorganisms, the extracellular matrix, and the surrounding environment.
Ana Barbosa   +4 more
semanticscholar   +1 more source

Introgression of Trifolium ambiguum Into Allotetraploid White Clover (Trifolium repens) Using the Ancestral Parent Trifolium occidentale as a Bridging Species

open access: yesFrontiers in Plant Science, 2022
White clover (Trifolium repens) is an allotetraploid pasture legume widely used in moist temperate climates, but its vulnerability to drought, grazing pressure and pests has restricted its wider use.
Ihsan Ullah   +8 more
doaj   +1 more source

FISH for All: A Fast and Efficient Fluorescent In situ Hybridization (FISH) Protocol for Marine Embryos and Larvae

open access: yesFrontiers in Physiology, 2022
In situ hybridization is one the most commonly used techniques for developmental and evolutionary biology and has extensively contributed to the identification of distinct cell types and cell states, as well dissecting several molecular mechanisms ...
Periklis Paganos   +5 more
semanticscholar   +1 more source

Histological Lesions and Replication Sites of PCV3 in Naturally Infected Pigs

open access: yesAnimals, 2021
Porcine circovirus type 3 (PCV3) has been recently described as a potential cause of abortions and systemic vasculitis in pigs. Although the virus has been detected by real-time PCR in several porcine tissues from countries worldwide, PCV3-associated ...
Elisa Rigo De Conti   +4 more
doaj   +1 more source

A technical review and guide to RNA fluorescence in situ hybridization

open access: yesPeerJ, 2020
RNA-fluorescence in situ hybridization (FISH) is a powerful tool to visualize target messenger RNA transcripts in cultured cells, tissue sections or whole-mount preparations.
Alexander P. Young, D. Jackson, R. Wyeth
semanticscholar   +1 more source

In Situ Hybridization of Feline Leukemia Virus in a Case of Osteochondromatosis

open access: yesVeterinary Sciences, 2022
Osteochondromatosis, also known as multiple cartilaginous exostosis, polyostotic osteochondroma, and multiple osteochondromas, comprises one-fifth of all primary bone tumors in cats, with no breed or sex predisposition or hereditary pattern.
Anna Szilasi   +4 more
doaj   +1 more source

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