Results 31 to 40 of about 2,969,693 (257)

In situ detection of mature microRNAs by labeled extension on ultramer templates

BioTechniques, 2009
We describe a new method for the in situ detection of a mature microRNA (miRNA) in formalin-fixed, paraffin-embedded tissues. The method involves the labeled extension of miRNA hybridized to an approximately 100-nucleotide–long ultramer template ...
Gerard J. Nuovo, Eun Joo Lee, Sean Lawler, Jakub Godlewski, Thomas D. Schmittgen   +4 more
doaj   +1 more source

Significance of molecular diagnostics in human papilloma virus (HPV) determination [PDF]

Archives of Biological Sciences, 2014
HPV infection is considered to be the most important etiologic factor in cervical cancer development. In this retrospective study, which included the period from 2000 to 2012, the results of two molecular techniques used in the detection of HPV ...
Kovačević G., Milošević V., Hrnjaković-Cvjetković I., Petrović V., Stefan-Mikić S., Radovanov J., Jovanović-Galović A., Patić A., Elez I.   +8 more
doaj   +1 more source

Rapid interphase and metaphase assessment of specific chromosomal changes in neuroectodermal tumor cells by in situ hybridization with chemically modified DNA probes [PDF]

, 1988
Repeated DNAs from the constitutive heterochromatin of human chromosomes 1 and 18 were used as probes in nonradioactive in situ hybridization experiments to define specific numerical and structural chromosome aberrations in three human glioma cell lines ...
A.H.N. Hopman, Atkin, Bigner, Brigati, Burns, Burns, Carine, Cavenee, Comings, Cooke, Cooke, Cremer, D. Tesin, Dale, Devilee, Devilee, Dudin, Durnam, Gosden, Hadlaczky, Hall, Henn, Higgins, Hopman, Hopman, Hopman, Hopman, Hopman, Joseph, Julien, Kinzler, Kohl, L. Manuelidis, Landegent, Landegent, Langer-Safer, Leary, Lund Jorgensen, Maniatis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, McDermid, Müller, Müncke, Pinkel, Pinkel, Rappold, Schardin, Schwab, Shapiro, Siegel, T. Cremer, Therman, Trask, Willard, Wu, Yang   +64 more
core   +1 more source

Step-by-step preparation of mouse eye sections for routine histology, immunofluorescence, and RNA in situ hybridization multiplexing

STAR Protocols, 2021
Summary: It can be challenging to maintain tissue integrity using established histology protocols. Here, we describe a protocol composed of Hartman’s fixation, window technique, microwave-based tissue processing, optimized depigmentation, and antigen ...
Ji Pang, Nancy Thomas, Dai Tsuchiya, Tari Parmely, Deyue Yan, Ting Xie, Yongfu Wang   +6 more
doaj  

Visualizing the Needle in the Haystack: In Situ Hybridization With Fluorescent Dendrimers

Biological Procedures Online, 2004
In situ hybridization with 3DNA™ dendrimers is a novel tool for detecting low levels of mRNA in tissue sections and whole embryos. Fluorescently labeled dendrimers were used to identify cells that express mRNA for the skeletal muscle transcription ...
Gerhart Jacquelyn, Baytion Michael, Perlman Jordanna, Neely Christine, Hearon Bridget, Nilsen Thor, Getts Robert, Kadushin James, George-Weinstein Mindy   +8 more
doaj   +1 more source

Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues

Biology Open, 2012
Summary Non-coding microRNA (miRNA) molecules bind their target mRNAs and thereby modulate the amount of protein produced. To understand the significance of a potential miRNA-mRNA interaction, temporal and spatial information on miRNA and mRNA expression
Anne Karine Lagendijk, Jon D. Moulton, Jeroen Bakkers   +2 more
doaj   +1 more source

Morphological detection of X- and Y-chromosomes in smears and paraffin-embedded tissues using a non-isotopic in situ hybridization technique (NISH) [PDF]

, 1994
Pharyngeal smears and paraffin-embedded tissue specimens (skeletal muscle, kidney) obtained from 10 male and 10 female individuals were evaluated using non-isotopic in situ hybridization (NISH) with commercial X- and Y-specific biotinylated probes which ...
Baretton, G., Betz, P., Eisenmenger, Wolfgang, Nerlich, A., Tübel, J.   +4 more
core   +1 more source

Fluorescent in situ hybridization (FISH) on the veterinary diagnostic field

Acta Fytotechnica et Zootechnica, 2016
Proceeding deals with of genomic changes detectable by FISH . The DSD syndrom in Yorkshire terrier 78,XY t (Y-;6p+) was observed by the use of X and Y FISH WCP probes. Following results indicated numerous genomic changes in cancers. Using comparative
Ján Dianovský, Beáta Holečková, Monika Drážovská, Katarína Šivíková, Viera Schwarzbacherová   +4 more
doaj   +1 more source

Historical perspective of in situ hybridization for the analysis of genomic constitution of plants [PDF]

, 2015
In situ hybridization involves hybridization of DNA or RNA probes to the cytological preparations. The technique originally used auto-radiographic labeling to map both repetitive and low copy DNA sequences.
Abbasi, FM, Inamullah, I, Khan, U, Masood, R, Perveen, F, Shah, M, Tabassum, S, Tariq Khan, M   +7 more
core   +2 more sources

Distribution of the Primary Endosymbiont (Candidatus Uzinura Diaspidicola) Within Host Insects from the Scale Insect Family Diaspididae

Insects, 2012
It has long been known that armored scale insects harbor endosymbiotic bacteria inside specialized cells called bacteriocytes. Originally, these endosymbionts were thought to be fungal symbionts but they are now known to be bacterial and have been named ...
Katharina Dittmar, Meghan Flarhety, Matthew E. Gruwell   +2 more
doaj   +1 more source