STAR Protocols, 2021 Summary: Visualizing precise spatial patterns of an organ-wide gene and protein expression among diverse cell types can provide critical insights into the fundamental processes underlying normal tissue homeostasis and disease development.
Rajendra Kumar Gurumurthy +2 more
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Fluorescence In Situ Hybridization [PDF]
Journal of Investigative Dermatology, 2013 Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect the presence or absence and location of specific gene sequences. It can visualize specific cytogenetic abnormalities (copy number aberrations) such as chromosomal deletion, amplification, and translocation. FISH has been used in prenatal diagnosis and has served both as
Chen, Amy Y.-Y., Chen, Andrew
openaire +2 more sources
Genome composition of 'Elatior'-begonias hybrids analyzed by genomic in situ hybridisation [PDF]
, 2010 Interspecific hybridization of various tuberous Begonia species hybrids with Begonia socotrana results in so-called 'Elatior'-begonias hybrids (B. x hiemalis Fotsch).
Laak, W.A. +3 more
core +2 more sources
Study of pregnant women with high risk of fetus abnormalities by routine cytogenetics method (karyotyping) and molecular method (FISH) by using X and Y probs and comparing the advantages and disadvantages of these methods in the northwest of Iran's patients [PDF]
مجله پزشکی دانشگاه علوم پزشکی تبریز, 2021 Background: The health of the fetus during the 9 months of pregnancy is very important for every pregnant couple. Identifying carriers of the genetic diseases and their diagnosis before birth, controls the disease's prevalence and does not impose huge ...
Parisa Vahidi +2 more
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Frontiers in Plant Science, 2020 Fluorescence in situ hybridization karyotypes have been widely used for evolutionary analysis on chromosome organization and genetic/genomic diversity in the wheat alliance (tribe Triticeae of Poaceae). The karyotpic diversity of Aegilops umbellulata, Ae.
Zhongping Song +7 more
semanticscholar +1 more source
Rapid metaphase and interphase detection of radiation-induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization [PDF]
, 1990 Chromosomal in situ suppression (CISS)-hybridization of biotinylated phage DNA-library inserts from sorted human chromosomes was used to decorate chromosomes 1 and 7 specifically from pter to qter and to detect structural aberrations of these chromosomes
Cremer, Christoph +4 more
core +1 more source
Biology Open, 2012 Summary Non-coding microRNA (miRNA) molecules bind their target mRNAs and thereby modulate the amount of protein produced. To understand the significance of a potential miRNA-mRNA interaction, temporal and spatial information on miRNA and mRNA expression
Anne Karine Lagendijk +2 more
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Quantification of virus-infected cells using RNA FISH-Flow
STAR Protocols, 2023 Summary: We present a protocol to detect cells that have been infected by RNA viruses. The method, RNA fluorescence in situ hybridization flow cytometry (RNA FISH-Flow), uses 48 fluorescently labeled DNA probes that hybridize in tandem to viral RNA.
Cody J. Warren +6 more
doaj +1 more source
Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs with radioactive and non-radioactive in situ hybridization techniques: diagnosis of trisomy 18 with probe L1.84 [PDF]
, 1986 The localization of chromosome 18 in human interphase nuclei is demonstrated by use of radioactive and nonradioactive in situ hybridization techniques with a DNA clone designated L1.84.
A. Brückner +33 more
core +1 more source
Fluorescent in situ hybridization (FISH) on the veterinary diagnostic field
Acta Fytotechnica et Zootechnica, 2016 Proceeding deals with of genomic changes detectable by FISH . The DSD syndrom in Yorkshire terrier 78,XY t (Y-;6p+) was observed by the use of X and Y FISH WCP probes. Following results indicated numerous genomic changes in cancers. Using comparative
Ján Dianovský +4 more
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