Results 41 to 50 of about 617,973 (397)

Protocol for the use of signal amplification by exchange reaction-fluorescence in situ hybridization on adult formalin-fixed paraffin-embedded mouse lung tissue

STAR Protocols, 2023
Summary: Fluorescence in situ hybridization (FISH) is a useful tool for analyzing RNA expression, but difficulties arise with low-abundance RNA and in tissues that are formalin-fixed paraffin-embedded (FFPE) because reagents can be expensive.
Rachel Warren, Aftab Shaik, Lauren Teubner, Handeng Lyu, Stijn De Langhe   +4 more
doaj   +1 more source

Towards Quantitative In Situ Hybridization [PDF]

Journal of Histochemistry & Cytochemistry, 1997
In situ hybridization analysis of tissue mRNA concentrations remains to be accepted as a quantitative technique, even though exposure of tissue sections to photographic emulsion is equivalent to Northern blot analysis. Because of the biological importance of in situ quantification of RNA sequences within a morphological context, we evaluated the ...
Jonker, A., de Boer, P.A.J., van den Hoff, M.J.B., Lamers, W.H., Moorman, A.F.M.   +4 more
openaire   +5 more sources

Distribution of the Primary Endosymbiont (Candidatus Uzinura Diaspidicola) Within Host Insects from the Scale Insect Family Diaspididae

Insects, 2012
It has long been known that armored scale insects harbor endosymbiotic bacteria inside specialized cells called bacteriocytes. Originally, these endosymbionts were thought to be fungal symbionts but they are now known to be bacterial and have been named ...
Katharina Dittmar, Meghan Flarhety, Matthew E. Gruwell   +2 more
doaj   +1 more source

In situ hybridization in paracoccidioidomycosis [PDF]

Medical Mycology, 1999
In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence.
T, De Brito, G S, Sandhu, B C, Kline, R A, Aleff, M P, Sandoval, R T, Santos, A A, Brandão, C S, Lacaz   +7 more
openaire   +2 more sources

Imaging of RNA in situ hybridization by atomic force microscopy [PDF]

, 1996
In this study we investigated the possibility of imaging internal cellular molecules after cytochemical detection with atomic force microscopy (AFM). To this end, rat 9G and HeLa cells were hybridized with haptenized probes for 28S ribosomal RNA, human ...
Corput, M.P.C. van de, Grooth, B.G. de, Kalle, W.H.J., Macville, M.V.E., Raap, A.K., Tanke, H.J.   +5 more
core   +3 more sources

Suboptimal concordance in testing and retesting results of triple-negative breast carcinoma cases among laboratories: one institution experience

Cancer Cell International, 2019
Background Triple-negative breast carcinoma (TNBC) patients do not benefit from hormone- or human epidermal growth factor receptor 2- (HER2-) targeted therapies. Accurate testing is pivotal for these patients. Methods TNBC cases that were retested at our
Jose De Jesus, Marilin Rosa
doaj   +1 more source

Molecular cytotaxonomy of primates by chromosomal in situ suppression hybridization [PDF]

, 1990
A new strategy for analyzing chromosomal evolution in primates is presented using chromosomal in situ suppression (CISS) hybridization. Biotin-labeled DNA libraries from flow-sorted human chromosomes are hybridized to chromosome preparations of ...
Anna Jauch, Bickmore, Cremer, Cremer, de Grouchy, Dutrillaux, Holmquist, Johannes Wienberg, Lalley, Lichter, Lichter, Lichter, O'Brien, Ohno, Pinkel, Roscoe Stanyon, Seuánez, Stanyon, Thomas Cremer, Van Dilla, Weber, Wienberg, Yunis   +22 more
core   +1 more source

Rapid interphase and metaphase assessment of specific chromosomal changes in neuroectodermal tumor cells by in situ hybridization with chemically modified DNA probes [PDF]

, 1988
Repeated DNAs from the constitutive heterochromatin of human chromosomes 1 and 18 were used as probes in nonradioactive in situ hybridization experiments to define specific numerical and structural chromosome aberrations in three human glioma cell lines ...
A.H.N. Hopman, Atkin, Bigner, Brigati, Burns, Burns, Carine, Cavenee, Comings, Cooke, Cooke, Cremer, D. Tesin, Dale, Devilee, Devilee, Dudin, Durnam, Gosden, Hadlaczky, Hall, Henn, Higgins, Hopman, Hopman, Hopman, Hopman, Hopman, Joseph, Julien, Kinzler, Kohl, L. Manuelidis, Landegent, Landegent, Langer-Safer, Leary, Lund Jorgensen, Maniatis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, Manuelidis, McDermid, Müller, Müncke, Pinkel, Pinkel, Rappold, Schardin, Schwab, Shapiro, Siegel, T. Cremer, Therman, Trask, Willard, Wu, Yang   +64 more
core   +1 more source

Role of dorsomedial striatum neuronal ensembles in incubation of methamphetamine craving after voluntary abstinence [PDF]

, 2017
We recently developed a rat model of incubation of methamphetamine craving after choice-based voluntary abstinence. Here, we studied the role of dorsolateral striatum (DLS) and dorsomedial striatum (DMS) in this incubation.
Bossert, Jennifer M., CAPRIOLI, DANIELE, Hope, Bruce T., Shaham, Yavin, Venniro, Marco, Warren, Brandon L., Zhang, Michelle   +6 more
core   +2 more sources

In Situ Gene Expression in Native Cryofixed Bone Tissue

Biomedicines, 2022
Bone is a very complex tissue that is constantly changing throughout the lifespan. The precise mechanism of bone regeneration remains poorly understood.
Krisztina Nikovics, Cédric Castellarin, Xavier Holy, Marjorie Durand, Halima Morin, Abdelhafid Bendahmane, Anne-Laure Favier   +6 more
doaj   +1 more source