Background Triple-negative breast carcinoma (TNBC) patients do not benefit from hormone- or human epidermal growth factor receptor 2- (HER2-) targeted therapies. Accurate testing is pivotal for these patients. Methods TNBC cases that were retested at our
Jose De Jesus, Marilin Rosa
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Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
A Gnirke+33 more
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In Situ Gene Expression in Native Cryofixed Bone Tissue
Bone is a very complex tissue that is constantly changing throughout the lifespan. The precise mechanism of bone regeneration remains poorly understood.
Krisztina Nikovics+6 more
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Molecular cytotaxonomy of primates by chromosomal in situ suppression hybridization [PDF]
A new strategy for analyzing chromosomal evolution in primates is presented using chromosomal in situ suppression (CISS) hybridization. Biotin-labeled DNA libraries from flow-sorted human chromosomes are hybridized to chromosome preparations of ...
Anna Jauch+22 more
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In Situ Hybridization Status of 568 Cases of HER2 Equivocal Breast Cancer
Objective To compare the differences between 2013 and 2018 ASCO/CAP HER2 testing guidelines in 568 immunohistochemical HER2 equivocal breast cancer patients and to analyze the accuracy and deficiency of dual-color silver enhanced in-situ hybridization ...
JIANG Yi'na+3 more
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Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization
Recent technical advances have made fluorescent in situ hybridization (ISH) a pivotal method to analyze neural tissue. In a highly sensitive ISH, it is important to reduce tissue autofluorescence.
Yousuke Tsuneoka+8 more
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Application of current in situ hybridization techniques for grape phylloxera (Daktulosphaira vitifoliae, Fitch) and grapevine (Vitis spp. L.) [PDF]
In situ hybridization and in situ PCR directly localize specific DNA and RNA sequences in tissues. To exactly focus on the processes occurring on cell- or tissue level, in situ techniques can be efficiently employed.
Blaich, R.+3 more
core +2 more sources
Imaging of RNA in situ hybridization by atomic force microscopy [PDF]
In this study we investigated the possibility of imaging internal cellular molecules after cytochemical detection with atomic force microscopy (AFM). To this end, rat 9G and HeLa cells were hybridized with haptenized probes for 28S ribosomal RNA, human ...
Corput, M.P.C. van de+5 more
core +3 more sources
A high-resolution anatomical framework of the neonatal mouse brain for managing gene expression data
This study aims to provide a high-resolution atlas and use it as an anatomical framework to localize the gene expression data for mouse brain on postnatal day 0 (P0).
Jyl Boline, Arthur W Toga
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Fluorescence in situ hybridization in hematology [PDF]
nema
Milošević Ivana+2 more
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