Results 1 to 10 of about 670,418 (378)

Phenotypic and functional characterization of corneal endothelial cells during in vitro expansion. [PDF]

Sci Rep, 2020
The advent of cell culture-based methods for the establishment and expansion of human corneal endothelial cells (CEnC) has provided a source of transplantable corneal endothelium, with a significant potential to challenge the one donor-one recipient ...
Frausto RF, Swamy VS, Peh GSL, Boere PM, Hanser EM, Chung DD, George BL, Morselli M, Kao L, Azimov R, Wu J, Pellegrini M, Kurtz I, Mehta JS, Aldave AJ.   +14 more
europepmc   +5 more sources

Tumor-Targeting Anti-CD20 Antibodies Mediate In Vitro Expansion of Memory Natural Killer Cells: Impact of CD16 Affinity Ligation Conditions and In Vivo Priming [PDF]

Frontiers in Immunology, 2018
Natural killer (NK) cells represent a pivotal player of innate anti-tumor immune responses. The impact of environmental factors in shaping the representativity of different NK cell subsets is increasingly appreciated.
Cristina Capuano, Simone Battella, Chiara Pighi, Lavinia Franchitti, Ombretta Turriziani, Stefania Morrone, Angela Santoni, Angela Santoni, Angela Santoni, Ricciarda Galandrini, Gabriella Palmieri   +10 more
doaj   +5 more sources

IL6 supports long-term expansion of hepatocytes in vitro

Nature Communications, 2022
Hepatocytes are very difficult to expand in vitro. Here the authors discover that IL6 promotes long-term expansion (>30 passages) of primary mouse hepatocytes in vitro by converting the cells into hepatic progenitor cells, which maintain full capacity of
Ren Guo, Mengmeng Jiang, Gang Wang, Bing Li, Xiaohui Jia, Yan Ai, Shanshan Chen, Peilan Tang, Aijie Liu, Qianting Yuan, Xin Xie   +10 more
doaj   +4 more sources

Development of a Chemically Defined Medium for in vitro Expansion of Primary Bovine Satellite Cells. [PDF]

Front Bioeng Biotechnol, 2022
The use of fetal bovine serum (FBS) in animal cell culture media is widely spread since it provides a broad spectrum of molecules that are known to support cell attachment and growth.
Kolkmann AM, Van Essen A, Post MJ, Moutsatsou P.   +3 more
europepmc   +2 more sources

Genetic profiling of human bone marrow mesenchymal stromal cells after in vitro expansion in clinical grade human platelet lysate. [PDF]

Front Bioeng Biotechnol, 2022
Mesenchymal stromal cells (MSCs) are non-hematopoietic cells that have a broad therapeutic potential. To obtain sufficient cells for clinical application, they must be expanded ex vivo. In the initial expansion protocols described, fetal calf serum (FCS)
De Becker A, Heestermans R, De Brouwer W, Bockstaele K, Maes K, Van Riet I.   +5 more
europepmc   +2 more sources

In Vitro Expansion of Human Gastric Epithelial Stem Cells and Their Responses to Bacterial Infection [PDF]

Gastroenterology, 2014
BACKGROUND & AIMS We previously established long-term, 3-dimensional culture of organoids from mouse tissues (intestine, stomach, pancreas, and liver) and human intestine and pancreas.
Sina Bartfeld, Tülay Bayram, Marc van de Wetering, Meritxell Huch, Harry Begthel, Pekka Kujala, Robert G. Vries, Peter J. Peters, Hans Clevers   +8 more
openalex   +2 more sources

In Vitro Culture Expansion Shifts the Immune Phenotype of Human Adipose-Derived Mesenchymal Stem Cells

Frontiers in Immunology, 2021
Human mesenchymal stem or stromal cells (hMSCs) are known for their potential in regenerative medicine due to their differentiation abilities, secretion of trophic factors, and regulation of immune responses in damaged tissues.
Richard Jeske, Xuegang Yuan, Qin Fu, Bruce A. Bunnell, Timothy M. Logan, Yan Li   +5 more
openalex   +2 more sources

Cord blood-derived V_δ2⁺ and V_δ2^- T cells acquire differential cell state compositions upon in vitro expansion. [PDF]

Sci Adv, 2023
Ng JWK, Tan KW, Guo DY, Lai JJH, Fan X, Poon Z, Lim TH, Lim AST, Lim TKH, Hwang WYK, Li S, Eaves CJ, Goh YT, Cheung AMS.   +13 more
europepmc   +3 more sources

Feasibility of Human Platelet Lysate as an Alternative to Foetal Bovine Serum for In Vitro Expansion of Chondrocytes. [PDF]

Int J Mol Sci, 2021
Liau LL, Hassan MNFB, Tang YL, Ng MH, Law JX.   +4 more
europepmc   +3 more sources

Fe-doped MOF nanoparticles: the LIFR and BMP4 dual-signaling pathways activated regulator for in vitro expansion of mouse embryonic stem cells. [PDF]

Mater Today Bio
Li Y, Wang H, Li Z, Wang H, Gao Y, Wu C, Wei B, Guo Z, Wang X, Jing G, Wang S.   +10 more
europepmc   +2 more sources