Results 91 to 100 of about 370,076 (257)

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Type I interferons modulate autophagy to shape gemcitabine response in pancreatic cancer cells

FEBS Open Bio, EarlyView.
Type I interferons differentially modulate autophagy and the response of pancreatic cancer cells to gemcitabine. IFNα2b stimulates autophagic flux and protects cells from gemcitabine‐induced cell death, contributing to chemoresistance. In contrast, IFNβ1a inhibits autophagosome formation and enhances gemcitabine‐induced cell death, resulting in ...
Lucy E. Bonilla, Martín M. Ledesma, Santiago A. Behr, Matías A. Pibuel, María L. Palanek, Daniel H. Grasso, Maria N. Garcia, Juan Garona, Silvina L. Lompardía, Elida Álvarez, Daniela L. Papademetrio   +10 more
wiley   +1 more source

A spheroid model that recapitulates the protective role of the lymph node microenvironment and serves as a platform for drug testing in chronic lymphocytic leukemia

HemaSphere
Chronic lymphocytic leukemia (CLL) B cells are characterized by a propensity to undergo rapid apoptosis when cultured in vitro, underscoring the importance of the tissue microenvironment for disease survival.
Elisa Lenti, Edoardo Visentin, Engin Bojnik, Alessia Neroni, Martina Franchino, Daniela Talarico, Nicolò Sacchetti, Lydia Scarfò, Aurora Maurizio, Jose Manuel Garcia‐Manteiga, Paolo Ghia, Andrea Brendolan   +11 more
doaj   +1 more source

In Vitro Assessment of Nintedanib in Keratoconus Corneal Stromal Microenvironment

Biomolecules
Keratoconus (KC) is a degenerative corneal disease, characterized by stromal thinning and abnormal ECM remodeling, leading to fibrosis. Corneal fibrosis is a leading cause of visual impairment.
Yasamin Moradi, Pawan Shrestha, Steve Mabry, Purnima Sharma, Karanpreet S. Multani, Kamran M. Riaz, Dimitrios Karamichos   +6 more
doaj   +1 more source

Selective elimination of quiescent cancer cells by dequalinium chloride via ROS‐mediated ferroptosis

FEBS Open Bio, EarlyView.
Dequalinium chloride (DQ) selectively eliminates quiescent cancer cells (QCCs) by amplifying mitochondrial ROS. QCCs exhibit elevated basal ROS and limited redox‐buffering capacity, making them vulnerable to further ROS increases. DQ‐induced ROS exceeds the tolerance threshold, triggering lipid peroxidation and ferroptosis, whereas proliferating cells ...
Kotaro Miyamoto, Shiori Sakai, Minori Endo, Terigele, Mizuho Niibori, Taiki Morita, Yu Goto, Arisa Yoshida, Tomohiro Tanaka, Ning Lin, Takahiro Kuchimaru, Hiroyuki Nakamura, Masahiro Inoue, Shinae Kizaka‐Kondoh, Tetsuya Kadonosono   +14 more
wiley   +1 more source

Adenosine Concentration Determination for in Vitro Tumor Microenvironment Simulation

Dose-Response
Objectives We aimed to investigate the concentration of adenosine (ADO) in the tumor microenvironment (TME), focusing on its potential to modulate tumor cells and natural killer (NK) cells, thereby facilitating tumor immune escape. Methods
Chun Chen, Chuanpeng Yang, Shuning Hu, Wenjie Nie, Sudan Ye, Minghao Lu, Xingjie Xu, Huajun Hu   +7 more
openaire   +3 more sources

In vitro phenotypically stable cartilage regeneration with mechanically adaptable microenvironment using a hydrostatic pressure bioreactor

Bioactive Materials
The regenerative repair of diverse cartilage injuries is a remarkable clinical challenge. Recently, hydrogel-based tissue engineering strategy offers an alternative treatment for cartilage defect repair.
Can Qiu, Yanhua Tang, Yunzhe Zhang, Tian An, Xuelan Chen, Shuang He, Zheng Ci, Wei Zhang, Huitang Xia, Yingying Huo, Di Wang, Guangdong Zhou, Yujie Hua   +12 more
doaj   +1 more source

Uncovering G Protein‐Coupled Receptors: Novel Targets and Biomarkers for Predicting Glioma Prognosis

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background Low‐grade gliomas (LGG) exhibit significant heterogeneity and recurrence risk. G protein‐coupled receptors (GPCR) contribute to glioma malignant progression, but their prognostic value remains unclear. This work attempts to formulate a GPCR‐based outcome‐predicting model for LGG. Methods Based on TCGA LGG data, the enrichment scores
Jun Yang, Dongxu Zhang, Dongyuan Liu, Da Lin, Hao Wang   +4 more
wiley   +1 more source

Reconstructing The Testicular Niche: Mechanisms and Translational Perspectives of Stem Cell-derived Testicular Organoids

Shengwu huaxue yu shengwu wuli jinzhan
Mammalian male germline development depends on a specialized gonadal somatic microenvironment. However, its in vitro reconstruction remains challenging.
DONG Xia, ZHOU Tai-Zeng, SHEN Lin-Yuan, ZHU Li, GAN Mai-Lin   +4 more
doaj   +1 more source