Results 171 to 180 of about 1,304,931 (266)

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Editorial: Integrating computational modeling and organoid technology for enhanced biological research

Frontiers in Bioengineering and Biotechnology
J. M. García-Aznar, J. M. García-Aznar, E. McEvoy, E. McEvoy, S. W. Verbruggen, P. Van Liedekerke   +5 more
doaj   +1 more source

TIM-1 and Tiny-TIM as Robust In Vitro Models for Oral Biopharmaceutics: Evidence from an International Ring Study. [PDF]

Pharmaceutics
O'Farrell C, Havenaar R, McAllister M, Hens B, Barker R, Mármol ÁL, Ansari A, Ooms T, Schilderink R, Schwabe R, Butler J, Stróžyk M, Martins Garcia T, Minekus D, Sarcevica I, Smith K, Tomaszewska I, Jones E, Batchelor H, Bellmann S.   +19 more
europepmc   +1 more source

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

Assessment of Chronic Myeloid Leukaemia In Vitro Models Variability: Insights Into Extracellular Vesicles. [PDF]

J Cell Mol Med
Mutti S, Cavalleri A, Federici S, Mangolini V, Paolini L, Bonvicini C, Ferraro RM, Mazzoldi EL, Garuffo L, Xhahysa B, Leoni A, Trenta F, Re F, Giliani SC, Avenoso D, Farina M, Malagola M, Russo D, Bernardi S.   +18 more
europepmc   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

In vitro models of microglia: a comparative study. [PDF]

Sci Rep
Woolf Z, Stevenson TJ, Lee K, Highet B, Macapagal Foliaki J, Ratiu R, Rustenhoven J, Correia J, Schweder P, Heppner P, Weinert M, Coppieters N, Park T, Montgomery J, Smith AM, Dragunow M.   +15 more
europepmc   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Post-Translational Modifications in Respiratory Virus Infection: Recent Insights into the Development of In Vitro Models. [PDF]

Int J Mol Sci
Ben Khlifa E, Campese A, Corsi A, Bombieri C, Romanelli MG, Valenti MT, Zipeto D, Castelli M, Lievens PM, Ruggiero A.   +9 more
europepmc   +1 more source