In vitro spermatogenesis: In search of fully defined conditions [PDF]
Frontiers in Cell and Developmental Biology, 2023 A complete reconstruction of spermatogenesis in vitro under fully defined conditions still has not been achieved. However, many techniques have been proposed to get closer to that aim. Here we review the current progress in the field.
A. Yu. Kulibin, E. A. Malolina
doaj +6 more sources
In vitro spermatogenesis in two‐dimensionally spread mouse testis tissues [PDF]
Reproductive Medicine and Biology, 2019 Purpose Mouse in vitro spermatogenesis is possible with classical organ culture methods, by placing the testis tissue at the interphase between culture medium and air.
Mitsuru Komeya +8 more
doaj +6 more sources
Generation of rat offspring using spermatids produced through in vitro spermatogenesis [PDF]
Scientific Reports, 2023 An in vitro spermatogenesis method using mouse testicular tissue to produce fertile sperm was established more than a decade ago. Although this culture method has generally not been effective in other animal species, we recently succeeded in improving ...
Takafumi Matsumura +11 more
doaj +4 more sources
Achievement of complete in vitro spermatogenesis in testicular tissues from prepubertal mice exposed to mono- or polychemotherapy [PDF]
Scientific Reports, 2022 The assessment of the impact of chemotherapies on in vitro spermatogenesis in experimental models is required before considering the application of this fertility restoration strategy to prepubertal boys who received these treatments before testicular ...
Marion Delessard +7 more
doaj +4 more sources
Discovering in vitro spermatogenesis stimulating factors. [PDF]
Cell Death Dis, 2015 Spermatozoan development encompasses pre-meiotic, meiotic and post-meiotic cellular processes adapted to genetically diversify and then vertically transmit haploid genomes via fertilization. Defined culture systems supporting spermatogonial stem cell differentiation through meiosis will provide long-sought-after experimental platforms to study ...
Chaudhary J, Hamra FK.
europepmc +5 more sources
In vitro spermatogenesis: A century‐long research journey, still half way around [PDF]
Reproductive Medicine and Biology, 2018 Background Spermatogenesis is one of the most complicated cellular differentiation processes in a body. Researchers struggled to find and develop a micro‐environmental condition that can support the process in vitro.
Mitsuru Komeya +2 more
doaj +4 more sources
Progress and future prospect of in vitro spermatogenesis. [PDF]
Oncotarget, 2017 Infertility has become a major health issue in the world. It affects the social life of couples and of all infertility cases; approximately 40-50% is due to "male factor" infertility. Male infertility could be due to genetic factors, environment or due to gonadotoxic treatment.
Ibtisham F +7 more
europepmc +5 more sources
Spermatogonial Stem Cells for In Vitro Spermatogenesis and In Vivo Restoration of Fertility [PDF]
Cells, 2020 Spermatogonial stem cells (SSCs) are the only adult stem cells capable of passing genes onto the next generation. SSCs also have the potential to provide important knowledge about stem cells in general and to offer critical in vitro and in vivo ...
Fahar Ibtisham, Ali Honaramooz
doaj +4 more sources
In vivo and in vitro spermatogenesis in prepubertal mouse testes exposed to low gonadotoxic doses of cytarabine or Daunorubicin [PDF]
Scientific ReportsThe development of experimental models treated by chemotherapy is needed for elucidating the side effects of cancer treatments administered prior to puberty on male gonad function and the feasibility of restoring fertility from exposed testicular tissues.
Marion Delessard +7 more
doaj +4 more sources
Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control [PDF]
The Journal of Reproduction and Development, 2023 Incorporation of bovine serum-derived albumin formulation (AlbuMAX) into a basic culture medium, MEMα, enables the completion of in vitro spermatogenesis through testicular tissue culture in mice.
Takehiko OGAWA +6 more
doaj +2 more sources