In Vitro Spermatogenesis in Explanted Adult Mouse Testis Tissues. [PDF]
PLoS ONE, 2015 Research on in vitro spermatogenesis is important for elucidating the spermatogenic mechanism. We previously developed an organ culture method which can support spermatogenesis from spermatogonial stem cells up to sperm formation using immature mouse ...
Takuya Sato +5 more
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A pumpless monolayer microfluidic device based on mesenchymal stem cell-conditioned medium promotes neonatal mouse in vitro spermatogenesis [PDF]
Stem Cell Research & Therapy, 2023 Background Childhood cancer treatment-induced gonadotoxicity causes permanent infertility/sub-infertility in nearly half of males. The current clinical and experimental approaches are limited to cryopreservation of prepubertal testicular strips and in ...
Selin Önen +6 more
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Recent Developments in In Vitro Spermatogenesis and Future Directions [PDF]
Reproductive Medicine, 2023 Recent developments in stem cell technologies have made significant advancements in the field of in vitro gametogenesis. In vitro gametogenesis (IVG) is a promising technology where functional gametes (sperm or egg cells) can be generated from stem cells.
In Ki Cho, Charles A. Easley
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In vitro spermatogenesis in isolated seminiferous tubules of immature mice. [PDF]
PLoS One, 2023 Mouse spermatogenesis, from spermatogonial stem cell proliferation to sperm formation, can be reproduced in vitro by culturing testis tissue masses of neonatal mice. However, it remains to be determined whether this method is also applicable when testis tissues are further divided into tiny fragments, such as segments of the seminiferous tubule (ST), a
Feng X +11 more
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Reverse transcriptase inhibitors enable the generation of fertile spermatids from fetal mouse testes in vitro [PDF]
Communications BiologyOrgan culture systems enabling in vitro spermatogenesis from neonatal mouse testes exist, but differentiation from fetal testes shortly after sex determination remains unsuccessful.
Mayuka Nishida +11 more
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Combination of knockout serum replacement and plasma rich in growth factors does not support in vitro spermatogenesis in mice [PDF]
Scientific ReportsCancer treatments can lead to infertility, particularly in prepubertal boys who cannot preserve sperm before therapy. In vitro spermatogenesis offers a promising strategy for fertility preservation in this population by enabling the development of sperm ...
Seyyed Amir Moradian +3 more
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Cryopreservation of testis tissues and in vitro spermatogenesis. [PDF]
Reprod Med Biol, 2016 AbstractCancer treatments, either chemo‐ or radiotherapy, may cause severe damage to gonads which could lead to the infertility of patients. In post‐pubertal male patients, semen cryopreservation is recommended to preserve the potential to have their own biological children in the future; however, it is not applicable to prepubertals.
Yokonishi T, Ogawa T.
europepmc +4 more sources
In-vitro spermatogenesis through testis modelling: Toward the generation of testicular organoids. [PDF]
Andrology, 2020 Richer G, Baert Y, Goossens E.
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Spermatogonial stem cells and in vitro spermatogenesis [PDF]
Reproductive Medicine and Biology, 2011 AbstractSpermatogonial stem cells (SSCs) provide the basis for the life‐long production of enormous numbers of sperm. The nature of these mysterious cells is being clarified. Although they were regarded to be mostly dormant, dividing rarely and remaining static in a niche, their rather dynamic behavior in the seminiferous tubules has been disclosed ...
Takehiko Ogawa
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Aquaculture ReportsMelatonin is a master neuroendocrine hormone in regulation of circadian rhythm and seasonal breeding behaviors in animal kingdom. Recently, we used the scaffold material to establish the three-dimension (3D) culture method, by which we found that ...
Jiajun Yao +4 more
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