Results 21 to 30 of about 25,424 (210)

In vitro mouse spermatogenesis with an organ culture method in chemically defined medium. [PDF]

PLoS ONE, 2018
We previously reported the successful induction and completion of mouse spermatogenesis by culturing neonatal testis tissues. The culture medium consisted of α-minimum essential medium (α-MEM), supplemented with Knockout serum replacement (KSR) or ...
Hiroyuki Sanjo, Mitsuru Komeya, Takuya Sato, Takeru Abe, Kumiko Katagiri, Hiroyuki Yamanaka, Yoko Ino, Noriaki Arakawa, Hisashi Hirano, Tatsuma Yao, Yuta Asayama, Akio Matsuhisa, Masahiro Yao, Takehiko Ogawa   +13 more
doaj   +3 more sources

Fact or fiction: In vitro spermatogenesis. [PDF]

Spermatogenesis, 2012
Many previous studies have aimed at spermatogenesis of male murine germ cells in vitro, but no efficient system has been established yet that covers the entire process of mammalian spermatogenesis in a culture dish permanently. In this review, we report on the requirements of spermatogenesis and the current state of different culture methods using ...
Reuter K, Schlatt S, Ehmcke J, Wistuba J.   +3 more
europepmc   +4 more sources

Development of the membrane ceiling method for in vitro spermatogenesis [PDF]

Scientific Reports
Spermatogenesis is one of the most complex processes of cell differentiation and its failure is a major cause of male infertility. Therefore, a proper model that recapitulates spermatogenesis in vitro has been long sought out for basic and clinical ...
Maki Kamoshita, Hiroki Shirai, Hiroko Nakamura, Tetsuya Kishimoto, Yuki Hatanaka, Daisuke Mashiko, Katsuhiro Esashika, Jingjing Yang, Satoshi Yamasaki, Takehiko Ogawa, Hiroshi Kimura, Masahito Ikawa   +11 more
doaj   +2 more sources

Spermatogonial Stem Cells and In Vitro Spermatogenesis: How Far Are We from a Human Testis on a Chip? [PDF]

Eur Urol Focus, 2023
Ramsoomair CK, Alver CG, Flannigan R, Ramasamy R, Agarwal A.   +4 more
europepmc   +3 more sources

Ex Vivo Spatiotemporal Characterization of Spermatogenesis in Mouse Testicular Organoids [PDF]

Advanced Science
Testicular organoids (TOs) offer an opportunity to preserve fertility, but current TO protocols are limited by suboptimal maintenance of the organoid structure, meiotic defects, and incomplete in vitro spermatogenesis (IVS). Here, a strategy is developed
Jiachen Sun, Lifa Zhu, Yutong Li, Jinyan Tang, Tao Zhang, Hengjia Zhang, Wanchu Wang, Lufan Li, Shuhui Bian, Xin Wu   +9 more
doaj   +2 more sources

In Vitro Spermatogenesis by Three-dimensional Culture of Spermatogonial Stem Cells on Decellularized Testicular Matrix. [PDF]

Galen Med J, 2019
Ashouri Movassagh S, Banitalebi Dehkordi M, Koruji M, Pourmand G, Farzaneh P, Ashouri Movassagh S, Jabari A, Samadian A, Khadivi F, Abbasi M.   +9 more
europepmc   +3 more sources

Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis. [PDF]

Stem Cell Res Ther, 2023
Salem M, Khadivi F, Javanbakht P, Mojaverrostami S, Abbasi M, Feizollahi N, Abbasi Y, Heidarian E, Rezaei Yazdi F.   +8 more
europepmc   +3 more sources

Artificial Gametogenesis and In Vitro Spermatogenesis: Emerging Strategies for the Treatment of Male Infertility. [PDF]

Int J Mol Sci
Kaltsas A, Kyrgiafini MA, Markou E, Koumenis A, Mamuris Z, Dimitriadis F, Zachariou A, Chrisofos M, Sofikitis N.   +8 more
europepmc   +2 more sources

Effectiveness of Voytik-Harbin Protocol in Fabrication of Ram's Testicular-Derived Hydrogel and Its Impact on Mouse In Vitro Spermatogenesis. [PDF]

Int J Fertil Steril
Rashki Ghaleno L, Rezaei Topraggaleh T, Montazeri L, Shahverdi A, Rezazadeh Valojerdi M.   +4 more
europepmc   +3 more sources

Roles of Spermatogonial Stem Cells in Spermatogenesis and Fertility Restoration

Frontiers in Endocrinology, 2022
Spermatogonial stem cells (SSCs) are a group of adult stem cells in the testis that serve as the foundation of continuous spermatogenesis and male fertility.
Lei Diao, Paul J. Turek, Constance M. John, Fang Fang, Renee A. Reijo Pera, Renee A. Reijo Pera   +5 more
doaj   +1 more source