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Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained [PDF]
Virology, 2014 The advent of recombinant DNA technology, development of infectious cDNA clones of RNA viruses, and reverse genetic technologies have revolutionized how viruses are studied.
Jianqiang Zhang +2 more
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Toward the Development of an Infectious cDNA Clone of a Human Enteric Coronavirus [PDF]
Advances in Experimental Medicine and Biology, 2006 respiratory, digestive, neurological, and immune-mediated diseases in human and animals. The outbreak of the severe acute respiratory syndrome (SARS) in 2003 was caused by SARS-CoV, which is most closely related to group II CoV, especially the bovine CoV (BCoV).
Yíngyún Caì
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Infectious Transcripts and cDNA Clones of RNA Viruses
Virology, 1994International ...
Boyer, Jean-Christophe, Haenni, A.L.
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Construction of an infectious cDNA clone of echovirus 6
Virus Research, 1992A complete cDNA copy of the echovirus 6 genome was constructed. Complementary DNA was reversed transcribed from viral RNA. Subgenomic cDNAs were obtained by direct cloning and polymerase chain reactions. Full length cDNA was constructed into the Bluescript II vector (pBSII) using unique, overlapping, restriction sites of four clones.
R V, Blackburn +2 more
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Construction of a replicon and an infectious cDNA clone of the Sofjin strain of the Far-Eastern subtype of tick-borne encephalitis virus [PDF]
Archives of Virology, 2011 Tick-borne encephalitis virus (TBEV) causes severe encephalitis in humans. The Sofjin-HO strain is the prototype strain of the TBEV Far-Eastern subtype and is highly pathogenic in a mouse model. In this study, we constructed replicons and infectious cDNA
Kentaro Yoshii +2 more
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Infectious cDNA clones of two grapevine viruses
Archives of Virology, 2000Full-length cDNA copies of the genomes of Grapevine virus A (GVA) and Grapevine virus B (GVB) under the control of bacteriophage T7 promoter have been synthesized, which were refractory to cloning in Escherichia coli. However, both transcribed cDNAs were infectious when mechanically inoculated to Nicotiana plants.
P, Saldarelli, M, Dell'Orco, A, Minafra
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An Infectious cDNA Clone of Porcine Reproductive and Respiratory Syndrome Virus
Advances in Experimental Medicine and Biology, 1998A plasmid containing a full-length cDNA copy of the Lelystad virus isolate (LV) of porcine reproductive and respiratory syndrome virus was constructed. When RNA that was transcribed in vitro from this full-length cDNA clone was transfected to BHK-21 cells, infectious LV was produced and secreted.
Gert Wensvoort +2 more
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Studying Poliovirus with Infectious Cloned cDNA
Clinical Infectious Diseases, 1984The application of molecular cloning to the study of poliovirus is discussed. Initially cDNA clones representing the entire genome of poliovirus type 1 (Mahoney) were prepared. These molecular clones were used for determining the complete nucleotide sequence of the viral genome. Subsequently a single cDNA clone representing the entire poliovirus genome
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Infectious in vitro transcripts from cloned cDNA of the potato A potyvirus
Virus Research, 1996A full-length cDNA copy of potato virus A RNA was constructed downstream from the bacteriophage T7 RNA polymerase promoter. A single extra guanosine not present in PVA RNA was added to the 5'-end of the cDNA. The capped in vitro transcripts from cloned cDNA were infectious in the mesophyll protoplasts and intact plants of Nicotiana tabacum.
, Puurand U +4 more
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Infectious RNA Transcripts from Grapevine Virus A cDNA Clone
Virus Genes, 1999A full length cDNA clone of grapevine virus A (GVA) was constructed downstream from the bacteriophage T7 RNA polymerase promoter. Capped in vitro-transcribed RNA was infectious in Nicotiana benthamiana and N. clevelandii plants. Symptoms induced by the RNA transcripts or by the parental virus were indistinguishable.
N, Galiakparov +3 more
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