Results 41 to 50 of about 695,844 (230)
Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging coronavirus infecting humans that is associated with acute pneumonia, occasional renal failure, and a high mortality rate and is considered a threat to public health. The construction
Fernando Almazán +7 more
doaj +1 more source
The recent outbreaks of Zika virus (ZIKV), its association with Guillain–Barré syndrome and fetal abnormalities, and the lack of approved vaccines and antivirals, highlight the importance of developing countermeasures to combat ZIKV disease.
Silvia Márquez-Jurado +5 more
doaj +1 more source
Infectious dimeric RNA transcripts are a powerful tool for reverse genetic analyses in viroid studies. However, the construction of dimeric cDNA clones is laborious and time consuming, especially in mutational analyses by in vitro mutagenesis.
Tatsuji Hataya, Takashi Naoi
doaj +1 more source
Primer sets used to generate infectious cDNA clone, pBRCVB3.
Underlined nt, restriction enzyme sites; overlined nt, T7 promoter; bold nt, viral sequence.Primer sets used to generate infectious cDNA clone, pBRCVB3.
Jean-Jack Riethoven (13854) +8 more
core +1 more source
Experiences with infectious cDNA clones of equine arteritis virus: Lessons learned and insights gained [PDF]
The advent of recombinant DNA technology, development of infectious cDNA clones of RNA viruses, and reverse genetic technologies have revolutionized how viruses are studied.
Zhang, Jianqiang +3 more
core +1 more source
Complete genome sequence and construction of an infectious full-length cDNA clone of a cucumber vein yellowing virus (CVYV) isolate from Portugal [PDF]
Cucumber vein yellowing virus (CVYV) is a member of the genus Ipomovirus in the family Potyviridae. In the National Center for Biotechnology Information (NCBI) database, three complete genome sequences of CVYV isolates from Spain (NC_006941), Israel ...
Rose, Hanna +4 more
core +1 more source
Dengue virus (DENV) is primarily transmitted by the bite of an infected mosquito of Aedes aegypti and Aedes albopictus, and symptoms caused may range from mild dengue fever to severe dengue hemorrhagic fever and dengue shock syndrome.
Mingyue Hu +8 more
doaj +1 more source
Reverse Genetics System for Rabbit vesivirus
Most caliciviruses are refractory to replication in cell culture and only a few members of the family propagate in vitro. Rabbit vesivirus (RaV) is unique due to its ability to grow to high titers in several animal and human cell lines.
Ángel L. Álvarez +8 more
doaj +1 more source
Infectious genomic RNA of Rhopalosiphum padi virus transcribed in vitro from a full-length cDNA clone [PDF]
Availability of a cloned genome from which infectious RNA can be transcribed is essential for investigating RNA virus molecular mechanisms. To date, no such clones have been reported for the Dicistroviridae, an emerging family of invertebrate viruses ...
Beckett, Randy J. +4 more
core +1 more source
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source

