Results 31 to 40 of about 695,844 (230)

Identification of an Arylnaphthalene Lignan Derivative as an Inhibitor against Dengue Virus Serotypes 1 to 4 (DENV-1 to -4) Using a Newly Developed DENV-3 Infectious Clone and Replicon

Microbiology Spectrum, 2023
Dengue virus (DENV) is the most widespread arbovirus, causing symptoms ranging from dengue fever to severe dengue, including hemorrhagic fever and shock syndrome.
Mingyue Hu, Wan-Fei Li, Tiantian Wu, Yang Yang, Guoquan Chen, Tongling Chen, Yongchen Liu, Yaqing Mei, De Wu, Youchuan Wei, Tingrong Luo, Hong-Jie Zhang, Yi-Ping Li   +12 more
doaj   +1 more source

Rescue of a genotype 4 human hepatitis E virus from cloned cDNA and characterization of intergenotypic chimeric viruses in cultured human liver cells and in pigs [PDF]

, 2012
Hepatitis E virus (HEV) is an important but extremely understudied human pathogen. Genotypes 1 and 2 are restricted to humans, whereas genotypes 3 and 4 are zoonotic, infecting both humans and pigs.
Meng, Xiang-Jin, Feagins, Alicia R, Huang, Yao-Wei, Córdoba, Laura, Dryman, Barbara A, Opriessnig, Tanja, Cossaboom, Caitlin M   +6 more
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In vitro assembly of an infectious cDNA clone of infectious bronchitis virus and its application as a gene transfer vector [PDF]

, 2005
An infectious cDNA clone of Vero cell adapted Beaudette strain of IBV was constructed using in vitro assembly of cDNA fragments. The entire genome of IBV was RT-PCR amplified into seven fragments, with each piece overlapping about 10 nucleotides.
Youn, Soonjeon
core   +2 more sources

Role of the conserved E2 residue G259 in classical swine fever virus production and replication

Virus Research, 2022
The E2 glycoprotein of classical swine fever virus (CSFV) plays multiple roles in the viral life cycle. The chimeric live attenuated C strain with the E2 substitution of bovine viral diarrhea virus (BVDV) is a promising marker vaccine candidate.
Weicheng Yi, Fengwei Zheng, Hongchang Zhu, Yihan Wu, Jiayang Wei, Zishu Pan   +5 more
doaj   +1 more source

A full-length infectious cDNA clone of a dsRNA totivirus-like virus

, 2022
Totivirus-like viruses are a group of non-segmented double-stranded (ds)RNA viruses with two open reading frames, which were recently discovered and provisionally assigned to the Totiviridae family.
Okamoto, Kenta, de Matos Filipe, Diogo,, de Matos Filipe, Diogo, Okamoto, Kenta,, Wang, Han, Wang, Han,   +5 more
core   +1 more source

Development and Characterization of a cDNA-Launch Recombinant Simian Hemorrhagic Fever Virus Expressing Enhanced Green Fluorescent Protein: ORF 2b’ Is Not Required for In Vitro Virus Replication

Viruses, 2021
Simian hemorrhagic fever virus (SHFV) causes acute, lethal disease in macaques. We developed a single-plasmid cDNA-launch infectious clone of SHFV (rSHFV) and modified the clone to rescue an enhanced green fluorescent protein-expressing rSHFV-eGFP that ...
Yingyun Cai, Shuiqing Yu, Ying Fang, Laura Bollinger, Yanhua Li, Michael Lauck, Elena N. Postnikova, Steven Mazur, Reed F. Johnson, Courtney L. Finch, Sheli R. Radoshitzky, Gustavo Palacios, Thomas C. Friedrich, Tony L. Goldberg, David H. O’Connor, Peter B. Jahrling, Jens H. Kuhn   +16 more
doaj   +1 more source

Establishment of a reverse genetics system for Schmallenberg virus, a newly emerged orthobunyavirus in Europe [PDF]

, 2013
Schmallenberg virus (SBV) is a newly emerged orthobunyavirus that has caused widespread disease in cattle, sheep and goats in Europe. Like other orthobunyaviruses, SBV is characterized by a tripartite negative-sense RNA genome that encodes four ...
Szemiel, A.M., McLees, A., Li, P., van Knippenberg, I.C., McLees, Angela, Li, Ping, Shi, X., van Knippenberg, Ingeborg C., Elliott, Richard M., Szemiel, Agnieszka M., Shi, Xiaohong, Koudriakova, E., Koudriakova, Elina, Blakqori, G., Elliott, R.M., Blakqori, Gjon   +15 more
core   +1 more source

Establishment of a Simple and Rapid Gene Delivery System for Cucurbits by Using Engineered of Zucchini yellow mosaic virus

The Plant Pathology Journal, 2016
The infectious full-length cDNA clone of zucchini yellow mosaic virus (ZYMV) isolate PA (pZYMV-PA), which was isolated from pumpkin, was constructed by utilizing viral transcription and processing signals to produce infectious in vivo transcripts. Simple
Minji Kang, Jang-Kyun Seo, Hoseong Choi, Hong-Soo Choi, Kook-Hyung Kim   +4 more
doaj   +1 more source

Home-made enzymatic premix and Illumina sequencing allow for one-step Gibson assembly and verification of virus infectious clones

Phytopathology Research, 2020
An unprecedented number of viruses have been discovered by leveraging advances in high-throughput sequencing. Infectious clone technology is a universal approach that facilitates the study of biology and role in disease of viruses.
Mingmin Zhao, Beatriz García, Araiz Gallo, Ioannis E. Tzanetakis, Carmen Simón-Mateo, Juan Antonio García, Fabio Pasin   +6 more
doaj   +1 more source

Hepatitis C virus p7 protein is crucial for assembly and release of infectious virions [PDF]

, 2007
Hepatitis C virus (HCV) infection is associated with chronic liver disease and currently affects about 3% of the world population. Although much has been learned about the function of individual viral proteins, the role of the HCV p7 protein in virus ...
Ralf Bartenschlager, Bartenschlager, R., Thomas Pietschmann, Kallis, S., Bartenschlager Ralf, Penin Francois, Penin, F., Patel, A.H., Pietschmann, T., Eike Steinmann, Francois Penin, Steinmann Eike, Steinmann, E., Arvind H Patel, Kallis Stephanie, Patel Arvind H, Stephanie Kallis, Pietschmann Thomas   +17 more
core   +1 more source