Results 51 to 60 of about 307,971 (276)

PCYT1A Regulates Phosphatidylcholine Homeostasis from the Inner Nuclear Membrane in Response to Membrane Stored Curvature Elastic Stress

Developmental Cell, 2018
Summary Cell and organelle membranes consist of a complex mixture of phospholipids (PLs) that determine their size, shape, and function. Phosphatidylcholine (PC) is the most abundant phospholipid in eukaryotic membranes, yet how cells sense and regulate ...
Afreen Haider, Yu-Chen Wei, Koini Lim, Antonio D Barbosa, Che-Hsiung Liu, U. Weber, M. Mlodzik, Kadri Oras, S. Collier, M. Hussain, Liang Dong, Satish Patel, Anna Alvarez-Guaita, V. Saudek, Benjamin J. Jenkins, A. Koulman, Marcus K. Dymond, R. Hardie, S. Siniossoglou, D. Savage   +19 more
semanticscholar   +1 more source

Targeted ablation of nesprin 1 and nesprin 2 from murine myocardium results in cardiomyopathy, altered nuclear morphology and inhibition of the biomechanical gene response.

PLoS Genetics, 2014
Recent interest has focused on the importance of the nucleus and associated nucleoskeleton in regulating changes in cardiac gene expression in response to biomechanical load.
Indroneal Banerjee, Jianlin Zhang, Thomas Moore-Morris, Emily Pfeiffer, Kyle S Buchholz, Ao Liu, Kunfu Ouyang, Matthew J Stroud, Larry Gerace, Sylvia M Evans, Andrew McCulloch, Ju Chen   +11 more
doaj   +1 more source

ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity

Nature Communications, 2018
Vesicle-mediated nucleocytoplasmic transport is a nuclear pore-independent mechanism for the nuclear export of macromolecular complexes, but the molecular basis for this transport remains largely unknown.
J. Arii, Mizuki Watanabe, Fumio Maeda, N. Tokai‐Nishizumi, T. Chihara, M. Miura, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, Y. Kawaguchi   +9 more
semanticscholar   +1 more source

Emerin caps the pointed end of actin filaments: evidence for an actin cortical network at the nuclear inner membrane.

PLoS Biology, 2004
X-linked Emery-Dreifuss muscular dystrophy is caused by loss of emerin, a LEM-domain protein of the nuclear inner membrane. To better understand emerin function, we used affinity chromatography to purify emerin-binding proteins from nuclear extracts of ...
James M Holaska, Amy K Kowalski, Katherine L Wilson   +2 more
doaj   +1 more source

The SUN protein Mps3 is required for spindle pole body insertion into the nuclear membrane and nuclear envelope homeostasis. [PDF]

PLoS Genetics, 2011
The budding yeast spindle pole body (SPB) is anchored in the nuclear envelope so that it can simultaneously nucleate both nuclear and cytoplasmic microtubules. During SPB duplication, the newly formed SPB is inserted into the nuclear membrane.
Jennifer M Friederichs, Suman Ghosh, Christine J Smoyer, Scott McCroskey, Brandon D Miller, Kyle J Weaver, Kym M Delventhal, Jay Unruh, Brian D Slaughter, Sue L Jaspersen   +9 more
doaj   +1 more source

Sun2 Is a Novel Mammalian Inner Nuclear Membrane Protein [PDF]

Journal of Biological Chemistry, 2004
Sun protein (Sun1 and Sun2) cDNAs were previously cloned based on the homology of their C-terminal regions (SUN (Sad1 and UNC) domain) with the Caenorhabditis elegans protein UNC-84 whose mutation disrupts nuclear migration/positioning. In this study, we raised an anti-Sun2 serum and identified Sun2 in mammalian cells.
Didier M, Hodzic, David B, Yeater, Luiza, Bengtsson, Henning, Otto, Philip D, Stahl   +4 more
openaire   +2 more sources

Inner/Outer Nuclear Membrane Fusion in Nuclear Pore Assembly [PDF]

Molecular Biology of the Cell, 2010
Nuclear pore complexes (NPCs) are large proteinaceous channels embedded in double nuclear membranes, which carry out nucleocytoplasmic exchange. The mechanism of nuclear pore assembly involves a unique challenge, as it requires creation of a long-lived membrane-lined channel connecting the inner and outer nuclear membranes.
Fichtman, Boris, Ramos, Corinne, Rasala, Beth, Harel, Amnon, Forbes, Douglass J.   +4 more
openaire   +2 more sources

Proteomic mapping by rapamycin-dependent targeting of APEX2 identifies binding partners of VAPB at the inner nuclear membrane

Journal of Biological Chemistry, 2019
Vesicle-associated membrane protein–associated protein B (VAPB) is a tail-anchored protein that is present at several contact sites of the endoplasmic reticulum (ER).
C. James, Marret Müller, M. Goldberg, C. Lenz, H. Urlaub, R. Kehlenbach   +5 more
semanticscholar   +1 more source

Protocol for single-molecule fluorescence recovery after photobleaching microscopy to analyze the dynamics and spatial locations of nuclear transmembrane proteins in live cells

STAR Protocols, 2021
Summary: Single-molecule fluorescence recovery after photobleaching (smFRAP) is a newly developed technique that combines single-molecule super-resolution microscopy and traditional FRAP microscopy.
Mark Tingey, Yichen Li, Weidong Yang
doaj   +1 more source

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source