Results 41 to 50 of about 199,615 (291)

Giardia Cyst Wall Protein 1 Is a Lectin That Binds to Curled Fibrils of the GalNAc Homopolymer [PDF]

open access: yes, 2010
The infectious and diagnostic stage of Giardia lamblia (also known as G. intestinalis or G. duodenalis) is the cyst. The Giardia cyst wall contains fibrils of a unique β-1,3-linked N-acetylgalactosamine (GalNAc) homopolymer and at least three cyst wall ...
Bullitt, Esther   +6 more
core   +5 more sources

Characterization of Stability and Small-scale Structural Differences of Intact Glycoprotein Complexes Using Native Mass Spectrometry Travelling-Wave Ion Mobility Spectrometry [PDF]

open access: yesZhipu Xuebao, 2022
Glycosylation is one of the most common types of post-translational modifications (PTMs) of proteins, which affects protein properties including conformation, stability and solubility, and plays essential roles in functions including molecular ...
XU Xiang1,2   +2 more
doaj   +1 more source

Ambient ionisation mass spectrometry for in situ analysis of intact proteins [PDF]

open access: yesJournal of Mass Spectrometry, 2018
AbstractAmbient surface mass spectrometry is an emerging field which shows great promise for the analysis of biomolecules directly from their biological substrate. In this article, we describe ambient ionisation mass spectrometry techniques for the in situ analysis of intact proteins.
Klaudia I. Kocurek   +2 more
openaire   +3 more sources

Intact cell MALDI-TOF mass spectrometric analysis of Chroococcidiopsis cyanobacteria for classification purposes and identification of possible marker proteins.

open access: yesPLoS ONE, 2018
Cyanobacteria represent a bacterial phyllum characteristic by the ability to photosynthesize. They are potentially applicable for the production of useful compounds but may also cause poisoning or at least health problems as they can produce cyanotoxins.
Marek Šebela   +4 more
doaj   +1 more source

Fluorescence Correlation Spectroscopy Reveals Efficient Cytosolic Delivery of Protein Cargo by Cell-Permeant Miniature Proteins. [PDF]

open access: yes, 2018
New methods for delivering proteins into the cytosol of mammalian cells are being reported at a rapid pace. Differentiating between these methods in a quantitative manner is difficult, however, as most assays for evaluating cytosolic protein delivery are
Knox, Susan L   +4 more
core   +2 more sources

Two-dimensional liquid chromatography protein expression mapping for differential proteomic analysis of normal and O157:H7 Escherichia coli

open access: yesBioTechniques, 2003
A multidimensional chromatographic method has been applied for the differential analysis of proteins from different strains of Escherichia coli bacteria.
Suping Zheng   +3 more
doaj   +1 more source

Unbiased protein association study on the public human proteome reveals biological connections between co-occurring protein pairs [PDF]

open access: yes, 2017
Mass-spectrometry-based, high-throughput proteomics experiments produce large amounts of data. While typically acquired to answer specific biological questions, these data can also be reused in orthogonal wayS to reveal new biological knowledge.
Gupta, Surya   +3 more
core   +2 more sources

Mass spectrometry for the evaluation of monoclonal proteins in multiple myeloma and related disorders: an International Myeloma Working Group Mass Spectrometry Committee Report

open access: yesBlood Cancer Journal, 2021
Plasma cell disorders (PCDs) are identified in the clinical lab by detecting the monoclonal immunoglobulin (M-protein) which they produce. Traditionally, serum protein electrophoresis methods have been utilized to detect and isotype M-proteins ...
David L. Murray   +12 more
doaj   +1 more source

Mass Spectrometry-based Methods for Phosphorylation Site Mapping of Hyperphosphorylated Proteins Applied to Net1, a Regulator of Exit from Mitosis in Yeast [PDF]

open access: yes, 2002
Prior to anaphase in Saccharomyces cerevisiae, Cdc14 protein phosphatase is sequestered within the nucleolus and inhibited by Net1, a component of the RENT complex in budding yeast. During anaphase the RENT complex disassembles, allowing Cdc14 to migrate
Annan, Roland S.   +5 more
core   +1 more source

Intact protein barcoding enables one-shot identification of CRISPRi strains and their metabolic state

open access: yesCell Reports: Methods
Summary: Detecting strain-specific barcodes with mass spectrometry can facilitate the screening of genetically engineered bacterial libraries. Here, we introduce intact protein barcoding, a method to measure protein-based library barcodes and metabolites
Vanessa Pahl   +4 more
doaj   +1 more source

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