Pervasive Suicidal Integrases in Deep-Sea Archaea
Mobile genetic elements (MGEs) often encode integrases which catalyze the site-specific insertion of their genetic information into the host genome and the reverse reaction of excision.
Catherine Badel+3 more
semanticscholar +1 more source
Directed DNA shuffling of retrovirus and retrotransposon integrase protein domains. [PDF]
Chimeric proteins are used to study protein domain functions and to recombine protein domains for novel or optimal functions. We used a library of chimeric integrase proteins to study DNA integration specificity.
Xiaojie Qi+6 more
doaj +1 more source
Positioning Diverse Type IV Structures and Functions Within Class 1 CRISPR-Cas Systems
Type IV CRISPR systems encode CRISPR associated (Cas)-like proteins that combine with small RNAs to form multi-subunit ribonucleoprotein complexes. However, the lack of Cas nucleases, integrases, and other genetic features commonly observed in most ...
Hannah N. Taylor+6 more
doaj +1 more source
Gene Loss and Horizontal Gene Transfer Contributed to the Genome Evolution of the Extreme Acidophile “Ferrovum” [PDF]
Indexación: Web of Science. Scopus.Acid mine drainage (AMD), associated with active and abandoned mining sites, is a habitat for acidophilic microorganisms that gain energy from the oxidation of reduced sulfur compounds and ferrous iron and that thrive ...
Daniel, Rolf+7 more
core +2 more sources
Permanent genetic memory with >1-byte capacity [PDF]
Genetic memory enables the recording of information in the DNA of living cells. Memory can record a transient environmental signal or cell state that is then recalled at a later time.
Yang, Lei+6 more
core +4 more sources
A trade-off between the fitness cost of functional integrases and long-term stability of integrons.
Horizontal gene transfer (HGT) plays a major role in bacterial microevolution as evident from the rapid emergence and spread of antimicrobial drug resistance.
Irina Starikova+7 more
doaj +1 more source
Mathematical model of a serine integrase-controlled toggle switch with a single input [PDF]
Dual-state genetic switches that can change their state in response to input signals can be used in synthetic biology to encode memory and control gene expression.
Colloms, Sean D.+3 more
core +1 more source
Serine integrase chimeras with activity in E. coli and HeLa cells
In recent years, application of serine integrases for genomic engineering has increased in popularity. The factor-independence and unidirectionality of these large serine recombinases makes them well suited for reactions such as site-directed vector ...
Alfonso P. Farruggio, Michele P. Calos
doaj +1 more source
Serine Integrases: Advancing Synthetic Biology.
Serine integrases catalyze precise rearrangement of DNA through site-specific recombination of small sequences of DNA called attachment (att) sites. Unlike other site-specific recombinases, the recombination reaction driven by serine integrases is highly
Christine A Merrick, Jia Zhao, S. Rosser
semanticscholar +1 more source
Functional Traits Co-Occurring with Mobile Genetic Elements in the Microbiome of the Atacama Desert [PDF]
Mobile genetic elements (MGEs) play an essential role in bacterial adaptation and evolution. These elements are enriched within bacterial communities from extreme environments. However, very little is known if specific genes co-occur with MGEs in extreme
Airo, Alessandro+4 more
core +1 more source