Results 31 to 40 of about 18,910 (292)

Gated rotation mechanism of site-specific recombination by ϕC31 integrase [PDF]

, 2012
Integrases, such as that of the Streptomyces temperate bacteriophage ϕC31, promote site-specific recombination between DNA sequences in the bacteriophage and bacterial genomes to integrate or excise the phage DNA.
A. R. McEwan, Brown, D. E. Buck, Dhar, Dhar, F. J. Olorunniji, Ghosh, Grainge, Grindley, Heichman, Kanaar, Khaleel, Kilbride, M. C. M. Smith, Rowland, S. D. Colloms, S. J. Rosser, Smith, Smith, Smith, Stark, Stark, Stark, Stark, Stark, Thorpe, W. M. Stark, Wasserman, Yuan   +28 more
core   +1 more source

New insights into the classification and integration specificity of streptococci integrative conjugative elements through extensive genome exploration

Frontiers in Microbiology, 2016
In this study, we searched for integrative conjugative elements (ICEs) in chromosomes of 124 strains of Streptococci. These exhaustive analyses revealed 105 putative ICEs and 26 slightly decayed elements that were grouped in 7 distinct families ...
Chloé eAmbroset, Chloé eAmbroset, Charles eColuzzi, Charles eColuzzi, Gérard eGuédon, Gérard eGuédon, Marie-Dominique eDevignes, Marie-Dominique eDevignes, Valentin eLoux, Thomas eLacroix, Sophie ePayot, Sophie ePayot, Nathalie eLeblond-Bourget, Nathalie eLeblond-Bourget   +13 more
doaj   +1 more source

Gene Loss and Horizontal Gene Transfer Contributed to the Genome Evolution of the Extreme Acidophile “Ferrovum” [PDF]

, 2016
Indexación: Web of Science. Scopus.Acid mine drainage (AMD), associated with active and abandoned mining sites, is a habitat for acidophilic microorganisms that gain energy from the oxidation of reduced sulfur compounds and ferrous iron and that thrive ...
Daniel, Rolf, González, Carolina, Holmes, David S., Mühling, Martin, Poehlein, Anja, Schlömann, Michael, Tischler, Judith S., Ullrich, Sophie R.   +7 more
core   +2 more sources

Directed DNA shuffling of retrovirus and retrotransposon integrase protein domains. [PDF]

PLoS ONE, 2013
Chimeric proteins are used to study protein domain functions and to recombine protein domains for novel or optimal functions. We used a library of chimeric integrase proteins to study DNA integration specificity.
Xiaojie Qi, Edwin Vargas, Liza Larsen, Whitney Knapp, G Wesley Hatfield, Richard Lathrop, Suzanne Sandmeyer   +6 more
doaj   +1 more source

Positioning Diverse Type IV Structures and Functions Within Class 1 CRISPR-Cas Systems

Frontiers in Microbiology, 2021
Type IV CRISPR systems encode CRISPR associated (Cas)-like proteins that combine with small RNAs to form multi-subunit ribonucleoprotein complexes. However, the lack of Cas nucleases, integrases, and other genetic features commonly observed in most ...
Hannah N. Taylor, Eric Laderman, Matt Armbrust, Thomson Hallmark, Dylan Keiser, Joseph Bondy-Denomy, Ryan N. Jackson   +6 more
doaj   +1 more source

Permanent genetic memory with >1-byte capacity [PDF]

, 2012
Genetic memory enables the recording of information in the DNA of living cells. Memory can record a transient environmental signal or cell state that is then recalled at a later time.
Yang, Lei, Fernandez-Rodriguez, Jesus, Nielsen, Alec Andrew, Lu, Timothy K., Voigt, Christopher A., McClune, Conor James, Laub, Michael T.   +6 more
core   +4 more sources

A trade-off between the fitness cost of functional integrases and long-term stability of integrons.

PLoS Pathogens, 2012
Horizontal gene transfer (HGT) plays a major role in bacterial microevolution as evident from the rapid emergence and spread of antimicrobial drug resistance.
Irina Starikova, Klaus Harms, Pål Haugen, Tracy T M Lunde, Raul Primicerio, Ørjan Samuelsen, Kaare M Nielsen, Pål J Johnsen   +7 more
doaj   +1 more source

Mathematical model of a serine integrase-controlled toggle switch with a single input [PDF]

, 2018
Dual-state genetic switches that can change their state in response to input signals can be used in synthetic biology to encode memory and control gene expression.
Colloms, Sean D., Ebenhöh, Oliver, Pokhilko, Alexandra, Stark, W. Marshall   +3 more
core   +1 more source

Serine integrase chimeras with activity in E. coli and HeLa cells

Biology Open, 2014
In recent years, application of serine integrases for genomic engineering has increased in popularity. The factor-independence and unidirectionality of these large serine recombinases makes them well suited for reactions such as site-directed vector ...
Alfonso P. Farruggio, Michele P. Calos
doaj   +1 more source

Functional Traits Co-Occurring with Mobile Genetic Elements in the Microbiome of the Atacama Desert [PDF]

, 2019
Mobile genetic elements (MGEs) play an essential role in bacterial adaptation and evolution. These elements are enriched within bacterial communities from extreme environments. However, very little is known if specific genes co-occur with MGEs in extreme
Airo, Alessandro, Schloter, Michael, Schulze-Makuch, Dirk, Sáenz, Johan S., Vestergaard, Gisle   +4 more
core   +1 more source