Results 31 to 40 of about 13,949 (234)
Directed DNA shuffling of retrovirus and retrotransposon integrase protein domains. [PDF]
PLoS ONE, 2013 Chimeric proteins are used to study protein domain functions and to recombine protein domains for novel or optimal functions. We used a library of chimeric integrase proteins to study DNA integration specificity.
Xiaojie Qi +6 more
doaj +1 more source
Molecular Microbiology, Volume 118, Issue 6, Page 652-669, December 2022., 2022 TnSmu1 is an integrative and conjugative element (ICE) (a type of mobile element that is transferred from cell to cell via conjugation) found in the genome of the oral pathogen Streptococcus mutans. We found that TnSmu1 is a functional ICE, capable of transfer to recipient cells that lack a copy of TnSmu1.
Lisa K. McLellan +2 more
wiley +1 more source
Positioning Diverse Type IV Structures and Functions Within Class 1 CRISPR-Cas Systems
Frontiers in Microbiology, 2021 Type IV CRISPR systems encode CRISPR associated (Cas)-like proteins that combine with small RNAs to form multi-subunit ribonucleoprotein complexes. However, the lack of Cas nucleases, integrases, and other genetic features commonly observed in most ...
Hannah N. Taylor +6 more
doaj +1 more source
A trade-off between the fitness cost of functional integrases and long-term stability of integrons.
PLoS Pathogens, 2012 Horizontal gene transfer (HGT) plays a major role in bacterial microevolution as evident from the rapid emergence and spread of antimicrobial drug resistance.
Irina Starikova +7 more
doaj +1 more source
Plant Biotechnology Journal, Volume 21, Issue 1, Page 78-96, January 2023., 2023 Summary Zanthoxylum armatum and Zanthoxylum bungeanum, known as ‘Chinese pepper’, are distinguished by their extraordinary complex genomes, phenotypic innovation of adaptive evolution and species‐special metabolites. Here, we report reference‐grade genomes of Z. armatum and Z. bungeanum.
Lisong Hu +20 more
wiley +1 more source
A population‐based temporal logic gate for timing and recording chemical events
Molecular Systems Biology, 2016 Engineered bacterial sensors have potential applications in human health monitoring, environmental chemical detection, and materials biosynthesis. While such bacterial devices have long been engineered to differentiate between combinations of inputs ...
Victoria Hsiao +3 more
doaj +1 more source
Serine integrase chimeras with activity in E. coli and HeLa cells
Biology Open, 2014 In recent years, application of serine integrases for genomic engineering has increased in popularity. The factor-independence and unidirectionality of these large serine recombinases makes them well suited for reactions such as site-directed vector ...
Alfonso P. Farruggio, Michele P. Calos
doaj +1 more source
Quantum-Chemistry based design of halobenzene derivatives with augmented affinities for the HIV-1 viral G4/C16 base-pair [PDF]
Front. Chem., 2020, 8, 440, 2019 The HIV-1 integrase (IN) is a major target for the design of novel anti-HIV inhibitors. Among these, three inhibitors which embody a halobenzene ring derivative (HR) in their structures are presently used in clinics. High-resolution X-ray crystallography of the complexes of the IN-viral DNA transient complex bound to each of the three inhibitors showed
arxiv +1 more source
Relationship between HIV integrase polymorphisms and integrase inhibitor susceptibility: An in silico analysis [PDF]
Heliyon, 2018 Integrase (IN) plays an essential role in HIV-1 replication, by mediating integration of the viral genome into the host cell genome. IN is a potential target of antiretroviral (ARV) therapeutic drugs such as ALLINI, Raltegravir (RAL), and Elvitegravir (EVG).
Hotma Martogi Lorensi Hutapea +2 more
openaire +4 more sources
The SSV1 viral integrase is not essential
Virology, 2007 Viral integration is a widely conserved characteristic in viruses in all domains of life; however, its necessity is not well understood in many cases. Integration using tyrosine recombinases is one of the most widespread and best characterized mechanisms of integration. We completely removed the tyrosine recombinase integrase from the hyperthermophilic
Adam J. Clore, Kenneth M. Stedman
openaire +3 more sources