Results 41 to 50 of about 9,111 (181)

Multi‐Targeting Non‐Specific Genome Engineering in Bacteria

Advanced Science, EarlyView.
In this study, we provide the first case to use the multi‐targeting integrase (MTI) systems in bacteria and develop a host‐independent generalizable approach, MNGE (Multi‐targeting Non‐specific Genome Engineering), for multi‐copy and random integration of metabolic genes or pathways in both Gram‐positive and Gram‐negative bacteria, which will ...
Runze Sun, Ruixiang You, Yiwen Zhou, Huiyan He, Wenfang Wang, Xudong Qu, Yinhua Lu, Lei Li   +7 more
wiley   +1 more source

Prevalence of SOS-mediated control of integron integrase expression as an adaptive trait of chromosomal and mobile integrons

Mobile DNA, 2011
Background Integrons are found in hundreds of environmental bacterial species, but are mainly known as the agents responsible for the capture and spread of antibiotic-resistance determinants between Gram-negative pathogens.
Cambray Guillaume, Sanchez-Alberola Neus, Campoy Susana, Guerin Émilie, Da Re Sandra, González-Zorn Bruno, Ploy Marie-Cécile, Barbé Jordi, Mazel Didier, Erill Ivan   +9 more
doaj   +1 more source

Casposase structure and the mechanistic link between DNA transposition and spacer acquisition by CRISPR-Cas

eLife, 2020
Key to CRISPR-Cas adaptive immunity is maintaining an ongoing record of invading nucleic acids, a process carried out by the Cas1-Cas2 complex that integrates short segments of foreign genetic material (spacers) into the CRISPR locus.
Alison B Hickman, Shweta Kailasan, Pavol Genzor, Astrid D Haase, Fred Dyda   +4 more
doaj   +1 more source

Ultrasound‐Responsive Engineered Bacteria for Targeted Cancer Therapy: Strategies, Mechanisms, and Applications

Advanced Science, EarlyView.
This review elucidates URB design strategies, including genetic and physicochemical modifications, while dissecting the synergistic mechanisms governing spatiotemporal control. It further evaluates applications in targeted drug delivery, gene modulation, and immunotherapy, finally offering perspectives on overcoming translational barriers for clinical ...
Xueyao Wang, Wenjing Xu, Yafang Lu, Huaibin Yu, Zhengbao Zha, Xijun Zhang   +5 more
wiley   +1 more source

Primary and promiscuous functions coexist during evolutionary innovation through whole protein domain acquisitions

eLife, 2020
Molecular examples of evolutionary innovation are scarce and generally involve point mutations. Innovation can occur through larger rearrangements, but here experimental data is extremely limited.
José Antonio Escudero, Aleksandra Nivina, Harry E Kemble, Céline Loot, Olivier Tenaillon, Didier Mazel   +5 more
doaj   +1 more source

The large plasmid carried class 1 integrons mediated multidrug resistance of foodborne Salmonella Indiana

Frontiers in Microbiology, 2022
Salmonella enterica serovar Indiana (S. Indiana) has aroused widespread concern as an important zoonotic pathogen. The molecular mechanism of multidrug resistance (MDR) in S. Indiana is not known and should be assessed.
Xuefeng Wang, Tian Wang, Mengjiao Guo, Chengcheng Zhang, Zongyi Bo, Yantao Wu, Guoxiang Chao   +6 more
doaj   +1 more source

Pharmacokinetics of dolutegravir, tenofovir and lamivudine during venous–venous extracorporeal membrane oxygenation (VV‐ECMO): A case report

British Journal of Clinical Pharmacology, EarlyView.
While providing potentially life‐saving cardiorespiratory support for critically ill patients, extracorporeal membrane oxygenation (ECMO) may detrimentally affect pharmacokinetic (PK) performance and concurrent drug efficacy and safety. We describe a patient with Pneumocystis jirovecii pneumonia (PJP) in the context of acquired immunodeficiency ...
Tom C. Zwart, Emiel Leegwater, Thomas H. Ottens, Emile F. Schippers, Erik B. Wilms, David M. Burger, Cees van Nieuwkoop, Liesbeth B. E. Bosma   +7 more
wiley   +1 more source

Substrate specificity of Ty1 integrase [PDF]

Journal of Virology, 1995
Integration of the Saccharomyces cerevisiae retrotransposon Ty1 requires the element-encoded integrase (IN) protein, which is a component of cytoplasmic virus-like particles (VLPs). Using purified recombinant Ty1 IN and an oligonucleotide integration assay based on Ty1 long terminal repeat sequences, we have compared IN activity on substrates having ...
S P, Moore, M, Powers, D J, Garfinkel
openaire   +2 more sources

Cardiovascular‐Kidney‐Metabolic Syndrome in People With HIV: An Emerging Frontier for Clinical Pharmacology

Clinical Pharmacology &Therapeutics, EarlyView.
As antiretroviral therapy (ART) prolongs lifespans, people with HIV (PWH) face a new syndemic: Cardiovascular‐Kidney‐Metabolic (CKM) syndrome. Yet CKM in PWH is poorly characterized. Inflammation, complex pharmacokinetic (PK) alterations, ART‐associated metabolic effects, and gut dysbiosis amplify risk. Managing CKM increases medication burden, thereby
Aaron S. Devanathan, Thomas D. Nolin
wiley   +1 more source

Attachment site selection and identity in Bxb1 serine integrase-mediated site-specific recombination. [PDF]

PLoS Genetics, 2013
Phage-encoded serine integrases mediate directionally regulated site-specific recombination between short attP and attB DNA sites without host factor requirements.
Shweta Singh, Pallavi Ghosh, Graham F Hatfull   +2 more
doaj   +1 more source