Results 51 to 60 of about 31,694 (314)

Serine integrase chimeras with activity in E. coli and HeLa cells

Biology Open, 2014
In recent years, application of serine integrases for genomic engineering has increased in popularity. The factor-independence and unidirectionality of these large serine recombinases makes them well suited for reactions such as site-directed vector ...
Alfonso P. Farruggio, Michele P. Calos
doaj   +1 more source

A New Freshwater Cyanosiphovirus Harboring Integrase [PDF]

Frontiers in Microbiology, 2018
Pelagic cyanobacteria are key players in the functioning of aquatic ecosystems, and their viruses (cyanophages) potentially affect the abundance and composition of cyanobacterial communities. Yet, there are few well-described freshwater cyanophages relative to their marine counterparts, and in general, few cyanosiphoviruses (family Siphoviridae) have ...
Kevin Xu Zhong, Kevin Xu Zhong, Curtis A. Suttle, Curtis A. Suttle, Curtis A. Suttle, Anne-Claire Baudoux, Evelyne Derelle, Jonathan Colombet, Anna Cho, Jessica Caleta, Christophe Six, Stéphan Jacquet   +11 more
openaire   +5 more sources

Quantum-Chemistry based design of halobenzene derivatives with augmented affinities for the HIV-1 viral G4/C16 base-pair [PDF]

Front. Chem., 2020, 8, 440, 2019
The HIV-1 integrase (IN) is a major target for the design of novel anti-HIV inhibitors. Among these, three inhibitors which embody a halobenzene ring derivative (HR) in their structures are presently used in clinics. High-resolution X-ray crystallography of the complexes of the IN-viral DNA transient complex bound to each of the three inhibitors showed
arxiv   +1 more source

Functional Traits Co-Occurring with Mobile Genetic Elements in the Microbiome of the Atacama Desert [PDF]

, 2019
Mobile genetic elements (MGEs) play an essential role in bacterial adaptation and evolution. These elements are enriched within bacterial communities from extreme environments. However, very little is known if specific genes co-occur with MGEs in extreme
Airo, Alessandro, Schloter, Michael, Schulze-Makuch, Dirk, Sáenz, Johan S., Vestergaard, Gisle   +4 more
core   +1 more source

The SSV1 viral integrase is not essential

Virology, 2007
Viral integration is a widely conserved characteristic in viruses in all domains of life; however, its necessity is not well understood in many cases. Integration using tyrosine recombinases is one of the most widespread and best characterized mechanisms of integration. We completely removed the tyrosine recombinase integrase from the hyperthermophilic
Adam J. Clore, Kenneth M. Stedman
openaire   +3 more sources

Integrating vectors for genetic studies in the rare Actinomycete Amycolatopsis marina

BMC Biotechnology, 2019
Background Few natural product pathways from rare Actinomycetes have been studied due to the difficulty in applying molecular approaches in these genetically intractable organisms.
Hong Gao, Buvani Murugesan, Janina Hoßbach, Stephanie K. Evans, W. Marshall Stark, Margaret C. M. Smith   +5 more
doaj   +1 more source

Influence of the amino-terminal sequence on the structure and function of HIV integrase

Retrovirology, 2020
Background Antiretroviral therapy (ART) can mitigate the morbidity and mortality caused by the human immunodeficiency virus (HIV). Successful development of ART can be accelerated by accurate structural and biochemical data on targets and their responses
Grant Eilers, Kushol Gupta, Audrey Allen, Jeffrey Zhou, Young Hwang, Michael B. Cory, Frederic D. Bushman, Gregory Van Duyne   +7 more
doaj   +1 more source

Horizontal DNA transfer mechanisms of bacteria as weapons of intragenomic conflict [PDF]

, 2016
Horizontal DNA transfer (HDT) is a pervasive mechanism of diversification in many microbial species, but its primary evolutionary role remains controversial.
A Adams, A Adams, AB Dalia, AI Khan, B Martin, BJ Haijema, BR Levin, C Boutry, C Buchrieser, C Camacho, C Canchaya, C Chewapreecha, C Fraser, C Johnston, C Johnston, C Johnston, Christophe Fraser, Christopher Wymant, CS Wylie, D Dubnau, D Hyatt, DA Baltrus, DA Caugant, DA Higgins, DA Morrison, DA Morrison, DE Fouts, DJP Engelmoer, E Borezee, E Szathmáry, E V Pestova, E Zaccaria, EA Campbell, EJ Feil, EJ Gaasbeek, EJ Gaasbeek, EP Rocha, ES Antonova, F Ginetti, F Griffith, F Liu, GG Wilson, GJ Stewart, GM Süel, GW Goldberg, H Bagci, H Bernstein, H Deveau, H Li, H Maamar, H Muller, H Ochman, H Tong, H-H Chou, HC Neu, I Chen, I Mortier-Barriere, I Mortier-Barrière, J Bondy-Denomy, J Guglielmini, J Majewski, J Majewski, J-P Claverys, JA Lanie, JA Londoño-Vallejo, JA Mongold, JC Lefevre, JC Mell, JH Stuy, JJ Sakamoto, JK Setlow, JM Auchtung, JM Musser, JM Smith, JM Smith, JO McInerney, JP Claverys, JP Claverys, JT Simpson, JW Beaber, K Turgay, KS Makarova, KT Konstantinidis, L Attaiech, L Fontaine, L Van Valen, L-M Bobay, LP Macfadyen, LS Frost, LS Håvarstein, LS Håvarstein, M Berge, M Boetzer, M Del Grosso, M Prudhomme, M Ramirez, MA Hoelzer, ME Kahn, MF Wojciechowski, MJ Lopez-Sanchez, ML Gwinn, MR Oggioni, MS Dorer, MTG Holden, Nicholas J. Croucher, Nick H. Barton, NJ Croucher, NJ Croucher, NJ Croucher, NJ Croucher, NJ Croucher, NJ Croucher, NJ Croucher, NJ Croucher, NJ Croucher, NJ Croucher, O Johnsborg, OE Cornejo, OH Ambur, OT Avery, P Glaser, P Jorth, P Luo, PA Levin, Paul Turner, PJ Johnsen, R Davidoff-Abelson, R Edgar, R Mostowy, RA Fisher, Rafal Mostowy, RE Michod, RE Yasbin, RJ Redfield, RJ Redfield, RJ Redfield, RJ Redfield, RJ Redfield, RJ Redfield, RK Aziz, RP Novick, S Lacks, SA McMahon, SB Beres, SE Finkel, SJ Labrie, SP Otto, Stephen D. Bentley, T Carver, T Nozawa, U Munoz-Najar, WA Sweetman, WJ Kent, X Charpentier, X Charpentier, X Didelot, Y Li, Z Zhou   +157 more
core   +3 more sources

Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer

Nature Communications, 2019
Retroviral integrases catalyze the insertion of viral DNA into the host cell DNA and can use nucleosomes as substrates for integration. Here the authors present the 3.9 Å cryo-EM structure of prototype foamy virus integrase after strand transfer into ...
Marcus D. Wilson, Ludovic Renault, Daniel P. Maskell, Mohamed Ghoneim, Valerie E. Pye, Andrea Nans, David S. Rueda, Peter Cherepanov, Alessandro Costa   +8 more
doaj   +1 more source

Genome integration and excision by a new Streptomyces bacteriophage, ϕJoe [PDF]

, 2017
Bacteriophages are the source of many valuable tools for molecular biology and genetic manipulation. In Streptomyces, most DNA cloning vectors are based on serine integrase site-specific DNA recombination systems derived from phage.
Fogg, Paul C M, Haley, Joshua A, Smith, Margaret C M, Stark, W Marshall   +3 more
core   +2 more sources