Results 51 to 60 of about 13,949 (234)

Synthesis of Pyrazolidin‐3‐one Derivatives Bearing Trifluoromethyl Group via Cascade Michael/Intramolecular Transamidation Reactions

Asian Journal of Organic Chemistry, EarlyView.
The cascade Michael/intramolecular transamidation reactions of 3‐phenacylidene‐2‐indolinone derivatives bearing a trifluoromethyl group as a novel Michael acceptor with hydrazines yielded the corresponding pyrazolidin‐3‐one derivatives in good to high yields with excellent diastereoselectivity.
Ryo Tsuyusaki, Kosuke Nakashima, Yasuyuki Matsushima, Shin‐ichi Hirashima, Tsuyoshi Miura   +4 more
wiley   +1 more source

IS21 family transposase cleaved donor complex traps two right-handed superhelical crossings

Nature Communications, 2023
Transposases are ubiquitous enzymes that catalyze DNA rearrangement events with broad impacts on gene expression, genome evolution, and the spread of drug-resistance in bacteria.
Mercedes Spínola-Amilibia, Lidia Araújo-Bazán, Álvaro de la Gándara, James M. Berger, Ernesto Arias-Palomo   +4 more
doaj   +1 more source

Multiple choices for HIV therapy with integrase strand transfer inhibitors

Retrovirology, 2012
Two integrases inhibitors, raltegravir and elvitegravir, have now been approved by regulatory agencies for use in the treatment of HIV-infected patients; and the approval of a third such drug, dolutegravir, is expected during 2013 on the basis of several
Raffi Francois, Wainberg Mark A
doaj   +1 more source

Casposase structure and the mechanistic link between DNA transposition and spacer acquisition by CRISPR-Cas

eLife, 2020
Key to CRISPR-Cas adaptive immunity is maintaining an ongoing record of invading nucleic acids, a process carried out by the Cas1-Cas2 complex that integrates short segments of foreign genetic material (spacers) into the CRISPR locus.
Alison B Hickman, Shweta Kailasan, Pavol Genzor, Astrid D Haase, Fred Dyda   +4 more
doaj   +1 more source

Unmasking the ancestral activity of integron integrases reveals a smooth evolutionary transition during functional innovation

Nature Communications, 2016
The integron integrases have evolved to perform recombination of single and double stranded DNA. Here the authors show that the ancestral pathway is still functional at double stranded sites, revealing the evolution towards the modern resolution pathway.
Jose Antonio Escudero, Celine Loot, Vincent Parissi, Aleksandra Nivina, Christiane Bouchier, Didier Mazel   +5 more
doaj   +1 more source

Effect of attC structure on cassette excision by integron integrases

Mobile DNA, 2011
Background Integrons are genetic elements able to integrate and disseminate genes as cassettes by a site-specific recombination mechanism. These elements contain a gene coding for an integrase that carries out recombination by interacting with two ...
Larouche André, Roy Paul H
doaj   +1 more source

Primary and promiscuous functions coexist during evolutionary innovation through whole protein domain acquisitions

eLife, 2020
Molecular examples of evolutionary innovation are scarce and generally involve point mutations. Innovation can occur through larger rearrangements, but here experimental data is extremely limited.
José Antonio Escudero, Aleksandra Nivina, Harry E Kemble, Céline Loot, Olivier Tenaillon, Didier Mazel   +5 more
doaj   +1 more source

Viral metagenomic analysis reveals potential biological hazards in non‐human primates in a zoo

Animal Research and One Health, EarlyView.
The investigation identifies the presence of Encephalomyocarditis virus (EMCV), Baboon endogenous virus (BAV), Human endogenous retrovirus (HERV), and Baboon endogenous virus (BaEV) in non‐human primates (NHPs) at the Zoo, indicating potential zoonotic threats.
Ruiying Liang, Xinming Tang, Lin Liang, Jiabo Ding, Ye Tian, Yixian Qin, Sufen Zhao, Lixia Zhang, Tianchun Pu, Xuefeng Liu, Jinpeng Liu, Chenglin Zhang, Zibin Li, Ting Jia   +13 more
wiley   +1 more source

The large plasmid carried class 1 integrons mediated multidrug resistance of foodborne Salmonella Indiana

Frontiers in Microbiology, 2022
Salmonella enterica serovar Indiana (S. Indiana) has aroused widespread concern as an important zoonotic pathogen. The molecular mechanism of multidrug resistance (MDR) in S. Indiana is not known and should be assessed.
Xuefeng Wang, Tian Wang, Mengjiao Guo, Chengcheng Zhang, Zongyi Bo, Yantao Wu, Guoxiang Chao   +6 more
doaj   +1 more source

Ginger DNA transposons in eukaryotes and their evolutionary relationships with long terminal repeat retrotransposons

Mobile DNA, 2010
Background In eukaryotes, long terminal repeat (LTR) retrotransposons such as Copia, BEL and Gypsy integrate their DNA copies into the host genome using a particular type of DDE transposase called integrase (INT).
Bao Weidong, Kapitonov Vladimir V, Jurka Jerzy   +2 more
doaj   +1 more source