Results 81 to 90 of about 31,694 (314)

Microbial Biosensor for Sensing and Treatment of Intestinal Inflammation

Advanced Science, EarlyView.
The engineered calprotectin biosensor detects gut inflammation, processes the signal through genetic regulation, and responds by expressing the reporter sfGFP and the therapeutic protein secIL10. This dual functionality enables the engineered biosensor not only to sense but also to mitigate gut inflammation, highlighting its potential for disease ...
Duolong Zhu, Jeffrey Galley, Jason Pizzini, Elena Musteata, Martin V. Douglas, Walter J. Chazin, Eric P. Skaar, Jeffrey J. Tabor, Robert A. Britton   +8 more
wiley   +1 more source

A novel family of integrases associated with prophages and genomic islands integrated within the tRNA-dihydrouridine synthase A (dusA) gene

Nucleic Acids Research, 2015
Genomic islands play a key role in prokaryotic genome plasticity. Genomic islands integrate into chromosomal loci such as transfer RNA genes and protein coding genes, whilst retaining various cargo genes that potentially bestow novel functions on the ...
Daniel N. Farrugia, Liam D. H. Elbourne, B. Mabbutt, I. Paulsen   +3 more
semanticscholar   +1 more source

Casposase structure and the mechanistic link between DNA transposition and spacer acquisition by CRISPR-Cas

eLife, 2020
Key to CRISPR-Cas adaptive immunity is maintaining an ongoing record of invading nucleic acids, a process carried out by the Cas1-Cas2 complex that integrates short segments of foreign genetic material (spacers) into the CRISPR locus.
Alison B Hickman, Shweta Kailasan, Pavol Genzor, Astrid D Haase, Fred Dyda   +4 more
doaj   +1 more source

Impact of Virus‐Mediated Modifications in Bacterial Communities on the Accumulation of Soil Organic Carbon

Advanced Science, EarlyView.
This study illustrates the impact of viruses on carbon cycling in soils with different availabilities of carbon. In conditions of low carbon availability, viruses adopt a lysogenic lifestyle, integrate into the host genomes and facilitate the degradation of carbon.
Mingfeng Liu, Guixiang Zhou, Congzhi Zhang, Lin Chen, Donghao Ma, Lijun Zhang, Chunhua Jia, Ling Ma, Jiabao Zhang   +8 more
wiley   +1 more source

Ginger DNA transposons in eukaryotes and their evolutionary relationships with long terminal repeat retrotransposons

Mobile DNA, 2010
Background In eukaryotes, long terminal repeat (LTR) retrotransposons such as Copia, BEL and Gypsy integrate their DNA copies into the host genome using a particular type of DDE transposase called integrase (INT).
Bao Weidong, Kapitonov Vladimir V, Jurka Jerzy   +2 more
doaj   +1 more source

Primary and promiscuous functions coexist during evolutionary innovation through whole protein domain acquisitions

eLife, 2020
Molecular examples of evolutionary innovation are scarce and generally involve point mutations. Innovation can occur through larger rearrangements, but here experimental data is extremely limited.
José Antonio Escudero, Aleksandra Nivina, Harry E Kemble, Céline Loot, Olivier Tenaillon, Didier Mazel   +5 more
doaj   +1 more source

The large plasmid carried class 1 integrons mediated multidrug resistance of foodborne Salmonella Indiana

Frontiers in Microbiology, 2022
Salmonella enterica serovar Indiana (S. Indiana) has aroused widespread concern as an important zoonotic pathogen. The molecular mechanism of multidrug resistance (MDR) in S. Indiana is not known and should be assessed.
Xuefeng Wang, Tian Wang, Mengjiao Guo, Chengcheng Zhang, Zongyi Bo, Yantao Wu, Guoxiang Chao   +6 more
doaj   +1 more source

Genome characteristics of facultatively symbiotic Frankia sp. strains reflect host range and host plant biogeography [PDF]

, 2007
Soil bacteria that also form mutualistic symbioses in plants encounter two major levels of selection. One occurs during adaptation to and survival in soil, and the other occurs in concert with host plant speciation and adaptation. Actinobacteria from the
Alloisio, Nicole, Bagnarol, Emilie, Bassi, Carla A., Berry, Alison M., Bickhart, Derek M., Choisne, Nathalie, Couloux, Arnaud, Cournoyer, Benoit, Cruveiller, Stephane, Daubin, Vincent, Demange, Nadia, Francino, Maria Pilar, Gogarten, Johann P., Goltsman, Eugene, Huang, Ying, Kopp, Olga R., Labarre, Laurent, Lapierre, Pascal, Normand, Philippe, Tisa, Louis S.   +19 more
core   +2 more sources

RAG-1 Mutations Associated with B-Cell-Negative SCID Dissociate the Nicking and Transesterification Steps of V(D)J Recombination [PDF]

, 2001
Some patients with B-cell-negative severe combined immune deficiency (SCID) carry mutations in RAG-1 or RAG-2 that impair V(D)J recombination. Two recessive RAG-1 mutations responsible for B-cell-negative SCID, R621H and E719K, impair V(D)J recombination
Chang, Fu-Chung, Desiderio, Stephen, Li, Wenhui   +2 more
core   +2 more sources

Brujita Integrase: A Simple, Arm-Less, Directionless, and Promiscuous Tyrosine Integrase System [PDF]

Journal of Molecular Biology, 2016
Mycobacteriophage Brujita is an unusual temperate phage in which establishment of superinfection immunity is dependent on chromosomal integration. Integration is mediated by a non-canonical tyrosine integrase (Int) lacking an N-terminal domain typically associated with binding to arm-type sites within the phage attachment site (attP).
Bryce L. Lunt, Graham F. Hatfull
openaire   +3 more sources