Results 251 to 260 of about 78,083 (294)

Initial crystallographic analysis of a recombinant human interleukin-1 receptor antagonist protein

Acta Crystallographica Section D Biological Crystallography, 1994
We report the crystallization of samples of a recombinant preparation of human interleukin-1 receptor antagonist protein (IRAP) and solution of the crystal structure by isomorphous replacement methods. Crystals were obtained by the hanging-drop vapor-diffusion method at 277 K from solutions of PEG 4000 containing sodium chloride, dithiothreitol and ...
L L, Clancy, B C, Finzel, A W, Yem, M R, Deibel, N A, Strakalaitis, D P, Brunner, R M, Sweet, H M, Einspahr   +7 more
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Cloning, heterologous expression and characterization of murine interleukin 1 receptor antagonist protein

European Journal of Immunology, 1991
AbstractA cDNA coding for the human interleukin 1 receptor antagonist protein (IL 1Ra) was used to clone the corresponding murine cDNA. The nucleotide sequence of the open reading frame coding for the processed form of mIL 1Ra predicted a 152‐residue protein that was 77% identical to human IL 1Ra.
M E, Shuck, T E, Eessalu, D E, Tracey, M J, Bienkowski   +3 more
openaire   +2 more sources

Cytokine regulation of the interleukin‐1 receptor antagonist protein in U937 cells

European Journal of Immunology, 1993
AbstractA naturally occurring receptor‐level antagonist of interleukin‐1 (IRAPorIL‐1 ra) has recently been cloned. To determine what stimuli might regulate this inhibitor, cytokines were tested for their effects on the steady‐state level of IRAP mRNA in phorbol ester‐differentiated U937 cells.
A E, Berger, D B, Carter, S O, Hankey, R N, McEwan   +3 more
openaire   +2 more sources

Interleukin-1 Receptor Antagonist Protein and mRNA in the Rat Adrenal Gland

Journal of Interferon & Cytokine Research, 1995
The occurrence of the endogenous receptor antagonist for the cytokine interleukin-1 in the rat adrenal gland was analyzed y polymerase chain reaction and by immunohistochemistry using a rabbit polyclonal antiserum. Expression of interleukin-1 receptor antagonist mRNA was demonstrated in both adrenal medulla and cortex, and a marked increase in the ...
M, Schultzberg, S, Tingsborg, S, Nobel, J, Lundkvist, S, Svenson, A, Simoncsits, T, Bartfai   +6 more
openaire   +2 more sources

Successful treatment of Erdheim-Chester disease by interleukin-1 receptor antagonist protein

Joint Bone Spine, 2014
Erdheim-Chester disease is a rare non-langerhans systemic histiocytosis of unknown origin, associated with bone diseases and severe visceral complications. Therapies have been disappointing. A recombinant form of interleukin-1 receptor antagonist (anakinra) has been used in a few cases when usual treatment fails.
Audrey, Courcoul, Emmanuelle, Vignot, Roland, Chapurlat   +2 more
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Purification of Human Recombinant Interleukin 1 Receptor Antagonist Proteins uponBacillus subtilisSporulation

Protein Expression and Purification, 1997
Human interleukin 1 receptor antagonist (IL-1ra) and IL-1ra mutants were constitutively expressed in recombinant Bacillus subtilis in endocellular and active form. In order to optimize the purification of the recombinant proteins, a new method has been developed.
G, Maurizi, V, Di Cioccio, G, Macchia, P, Bossù, C, Bizzarri, U, Visconti, D, Boraschi, A, Tagliabue, P, Ruggiero   +8 more
openaire   +2 more sources

Treatment With an Interleukin-1 Receptor Antagonist Protein Prolongs Mouse Islet Allograft Survival

Diabetes, 1993
An interleukin-1 receptor antagonist protein was evaluated with regard to its efficacy in allogeneic and xenogeneic islet transplantation. Alloxan-induced diabetic C57BL/6 (H-2b) mice were transplanted under the kidney capsule with 500 C57BL/Ks (H-2d) mouse islets.
Sandberg, J O, Eizirik, Decio L., Sandler, S, Tracey, D E, Andersson, A.   +4 more
openaire   +2 more sources

The mouse interleukin 1 receptor antagonist protein: gene structure and regulation in vitro

Cytokine, 1994
The interleukin 1 receptor antagonist (IL-1ra) protein is an inhibitor of the pro-inflammatory cytokine interleukin 1. We have sequenced the mouse gene encoding the monocyte form of IL-1ra (IL-1rn) and compared it with the sequence of the human homologue.
K A, Zahedi, C M, Uhlar, M, Rits, A E, Prada, A S, Whitehead   +4 more
openaire   +2 more sources

Purification, cloning, expression and biological characterization of an interleukin-1 receptor antagonist protein

Nature, 1990
A human myelomonocytic cell line, U937, produced an interleukin-1 (IL-1) receptor antagonist protein (IRAP) which was purified and partially sequenced. A complementary DNA coding for IRAP was cloned and sequenced. The mature translation product of the cDNA has been expressed in Escherichia coli and was an active competitive inhibitor of the binding of ...
D. B. Carter, M. R. Deibel, C. J. Dunn, C.-S. C. Tomich, A. L. Laborde, J. L. Slightom, A. E. Berger, M. J. Bienkowski, F. F. Sun, R. N. McEwan, P. K. W. Harris, A. W. Yem, G. A. Waszak, J. G. Chosay, L. C. Sieu, M. M. Hardee, H. A. Zurcher-Neely, I. M. Reardon, R. L. Heinrikson, S. E. Truesdell, J. A. Shelly, T. E. Eessalu, B. M. Taylor, D. E. Tracey   +23 more
openaire   +2 more sources

Recombinant interleukin-1 receptor antagonist protein prevents sensitization and intestinal anaphylaxis in Guinea pigs

Life Sciences, 1993
Recombinant interleukin-1 receptor antagonist protein (rlRAP, 0.5 mg/kg) administered intraperitoneally in guinea pigs one hour before primary and booster parenteral sensitization (1 ml) by cow milk, led to a reduced immunoglobulin E (IgE) production, as displayed by a passive cutaneous anaphylaxis test.
Theodorou, Vassilia, Fioramonti, Jean, Bueno, Lionel   +2 more
openaire   +3 more sources