Assembly mechanisms of the bacterial cytoskeletal protein FilP
Life Science Alliance, 2019 FilP is a coiled-coil protein that self-assembles into striated filaments and meshwork that shares structural and biochemical characteristics with metazoan intermediate filaments. Despite low-sequence homology, the intermediate filament (IF)–like protein
Ala Javadi +4 more
doaj +1 more source
Reconstitution of cytolinker-mediated crosstalk between actin and vimentin
European Journal of Cell BiologyCell shape and motility are determined by the cytoskeleton, an interpenetrating network of actin filaments, microtubules, and intermediate filaments. The biophysical properties of each filament type individually have been studied extensively by cell-free
Irene Istúriz Petitjean +6 more
doaj +1 more source
Molecular Neurodegeneration, 2019 Many neurodegenerative disorders, including Parkinson’s, Alzheimer’s, and amyotrophic lateral sclerosis, are well known to involve the accumulation of disease-specific proteins.
Alessandro Didonna, Puneet Opal
doaj +1 more source
Tmod1 and CP49 synergize to control the fiber cell geometry, transparency, and mechanical stiffness of the mouse lens. [PDF]
PLoS ONE, 2012 The basis for mammalian lens fiber cell organization, transparency, and biomechanical properties has contributions from two specialized cytoskeletal systems: the spectrin-actin membrane skeleton and beaded filament cytoskeleton.
David S Gokhin +7 more
doaj +1 more source
Isolation of the intermediate filament protein vimentin by chromatofocusing [PDF]
FEBS Letters, 1985 A novel, simple and relatively rapid method is described for the isolation of the intermediate‐sized filament protein vimentin from eye lens tissue. Chromatofocusing is applied as the sole purification step. The apparent isoelectric point of the protein in 6 M urea and at 22°C is 4.9.
Bloemendal, Hans +3 more
openaire +2 more sources
Intermediate filaments take the heat as stress proteins [PDF]
Trends in Cell Biology, 2010 Intermediate filament (IF) proteins and heat shock proteins (HSPs) are large multimember families that share several features, including protein abundance, significant upregulation in response to a variety of stresses, cytoprotective functions, and the phenocopying of several human diseases after IF protein or HSP mutation.
D M, Toivola +3 more
openaire +2 more sources
Intermediate filament linker proteins.
Society of General Physiologists series, 1997 Soc Gen Physiol ...
Fuchs, E. +6 more
openaire +2 more sources
Intermediate Filaments in Motion: Observations of Intermediate Filaments in Cells Using Green Fluorescent Protein-Vimentin [PDF]
Molecular Biology of the Cell, 1999 In the movies presented in this essay we have demonstrated that IFs are dynamic in nature. Movements of IFs include both extension and retraction and are dependent on the presence of intact MTs. Furthermore, we have characterized a possible intermediate of IF turnover, IF fragments.
J L, Martys +3 more
openaire +2 more sources
Multiple sites in αB-crystallin modulate its interactions with desmin filaments assembled in vitro. [PDF]
PLoS ONE, 2011 The β3- and β8-strands and C-terminal residues 155-165 of αB-crystallin were identified by pin arrays as interaction sites for various client proteins including the intermediate filament protein desmin.
Scott A Houck +3 more
doaj +1 more source
Septins as membrane influencers: direct play or in association with other cytoskeleton partners
Frontiers in Cell and Developmental Biology, 2023 The cytoskeleton comprises three polymerizing structures that have been studied for a long time, actin microfilaments, microtubules and intermediate filaments, plus more recently investigated dynamic assemblies like septins or the endocytic-sorting ...
Béatrice Benoit +3 more
doaj +1 more source