Results 91 to 100 of about 1,101,834 (300)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Palladium-catalyzed remote internal C(sp 3)−H bond chlorination of alkenes

Nature Communications
C(sp 3)–Cl bonds are present in numerous biologically active molecules and can also be used as a site for diversification by substitution or cross-coupling reactions.
Yao-Xin Wang, Zhen Wang, Xiao-Hui Yang
doaj   +1 more source

PENGARUH BAHAN PENGAWETAN PHOXIM TERHADAP SIFAT PAPAN PARTIKEL KAYU KARET (hevea brasiliensis) (effect of phoxim preservation on the properties of rubber wood particleboard (hevea brasiliensis))

Jurnal Penelitian Hasil Hutan (Journal of Forest Products Research), 2017
The objectives of the study was to determine the effect of phoxim on the properties of rubber wood particleboard (Hevea brasiliensis ) phoxim concentration used were 0.05,1, 1.5 and 2 % based on particle dry weight.
Rodjak Memed, I M Sulastiningsih, Paribotro Sutigno   +2 more
doaj   +1 more source

Theory for structure and bulk-modulus determination

, 2003
A new method for direct evaluation of both crystalline structure, bulk modulus B_0, and bulk-modulus pressure derivative B'_0 of solid materials with complex crystal structures is presented.
Schroder, Elsebeth, Ziambaras, Eleni
core   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Investigation on the bond-slip behavior of recycled aggregate concrete-filled steel tube with studs.

PLoS ONE
This study investigates the influence of built-in studs on the bond behavior in recycled aggregate concrete-filled steel tube (RACFST) composite structures through push-out experiments.
Gehao Cai, Bing Sun, Sheng Zeng, Peng Yang, Jie Zhang   +4 more
doaj   +1 more source

Fluctuating hydrogen-bond network of the Hv1 ion channel

Computational and Structural Biotechnology Journal
The human voltage-gated proton channel Hv1 has an intriguing architecture, as (i) its voltage-sensing unit contributes to the proton permeation pathway and (ii) the single channel proton flux is too large to be supplied solely by diffusion from the ...
Honey Jain, Michalis Lazaratos, Peter Pohl, Ana-Nicoleta Bondar   +3 more
doaj   +1 more source

Rydberg trimers and excited dimers bound by internal quantum reflection

, 2009
Quantum reflection is a pure wave phenomena that predicts reflection of a particle at a changing potential for cases where complete transmission occurs classically.
B. Butscher, J. B. Balewski, J. M. Rost, J. Nipper, J. P. Shaffer, J. Stanojevic, M. Büttiker, R. Löw, S. P. Andreev, T. Pfau, T. Pohl, V. Bendkowsky, W. Li   +12 more
core   +1 more source