Results 41 to 50 of about 16,625 (164)

Laser microdissection-based analysis of the Y sex chromosome of the Antarctic fish Chionodraco hamatus (Notothenioidei, Channichthyidae)

Comparative Cytogenetics, 2015
Microdissection, DOP-PCR amplification and microcloning were used to study the large Y chromosome of Chionodraco hamatus, an Antarctic fish belonging to the Notothenioidei, the dominant component of the Southern Ocean fauna.
Ennio Cocca, Agnese Petraccioli, Maria Alessandra Morescalchi, Gaetano Odierna, Teresa Capriglione   +4 more
doaj   +1 more source

Targeted gene knockin in zebrafish using the 28S rDNA-specific non-LTR-retrotransposon R2Ol

Mobile DNA, 2019
Background Although most of long interspersed elements (LINEs), one class of non-LTR-retrotransposons, are integrated into the host genome randomely, some elements are retrotransposed into the specific sequences of the genomic regions, such as rRNA gene (
Azusa Kuroki-Kami, Narisu Nichuguti, Haruka Yatabe, Sayaka Mizuno, Shoji Kawamura, Haruhiko Fujiwara   +5 more
doaj   +1 more source

A single molecule scaffold for the maize genome. [PDF]

PLoS Genetics, 2009
About 85% of the maize genome consists of highly repetitive sequences that are interspersed by low-copy, gene-coding sequences. The maize community has dealt with this genomic complexity by the construction of an integrated genetic and physical map (iMap)
Shiguo Zhou, Fusheng Wei, John Nguyen, Mike Bechner, Konstantinos Potamousis, Steve Goldstein, Louise Pape, Michael R Mehan, Chris Churas, Shiran Pasternak, Dan K Forrest, Roger Wise, Doreen Ware, Rod A Wing, Michael S Waterman, Miron Livny, David C Schwartz   +16 more
doaj   +1 more source

Interspersed repetitive and tandemly repetitive sequences are differentially represented in extrachromosomal covalently closed circular DNA of human diploid fibroblasts [PDF]

Nucleic Acids Research, 1985
Extrachromosomal covalently closed circular DNA (cccDNA) was isolated from human diploid fibroblasts by alkaline denaturation/renaturation and CsCl-ethidium bromide isopycnic centrifugation. Probing across these gradient fractions showed a higher proportion of cccDNA sequences homologous to the interspersed highly repetitive Alu I and Kpn I sequences ...
K, Riabowol, R J, Shmookler Reis, S, Goldstein   +2 more
openaire   +2 more sources

High-Throughput Scanning of the Rat Genome Using Interspersed Repetitive Sequence-PCR Markers

Genomics, 2000
We report the establishment of a hybridization-based marker system for the rat genome based on the PCR amplification of interspersed repetitive sequences (IRS). Overall, 351 IRS markers were mapped within the rat genome. The IRS marker panel consists of 210 nonpolymorphic and 141 polymorphic markers that were screened for presence/absence polymorphism ...
Gosele, C, Hong, L, Kreitler, T, Rossmann, M, Hieke, B, Gross, U, Kramer, M, Himmelbauer, H, Bihoreau, M, Kwitek-Black, A, Twigger, S, Tonellato, P, Jacob, H, Schalkwyk, L, Lindpaintner, K, Ganten, D, Lehrach, H, Knoblauch, M   +17 more
openaire   +3 more sources

Droplet digital PCR for the quantification of Alu methylation status in hematological malignancies

Diagnostic Pathology, 2018
Background Alu repeats, belonging to the Short Interspersed Repetitive Elements (SINEs) class, contain about 25% of CpG sites in the human genome. Alu sequences lie in gene-rich regions, so their methylation is an important transcriptional regulation ...
Paola Orsini, Luciana Impera, Elisa Parciante, Cosimo Cumbo, Crescenzio F. Minervini, Angela Minervini, Antonella Zagaria, Luisa Anelli, Nicoletta Coccaro, Paola Casieri, Giuseppina Tota, Claudia Brunetti, Alessandra Ricco, Paola Carluccio, Giorgina Specchia, Francesco Albano   +15 more
doaj   +1 more source

Interspersed repetitive sequence (IRS)-PCR for typing of whole genome radiation hybrid panels

Nucleic Acids Research, 2000
The typing of a radiation hybrid (RH) panel is generally achieved using a unique primer pair for each marker. We here describe a complementing approach utilizing IRS-PCR. Advantages of this technology include the use of a single universal primer to specify any locus, the rapid typing of RH lines by hybridization, and the conservative use of hybrid DNA.
Himmelbauer, H, Schalkwyk, L, Lehrach, H
openaire   +3 more sources

Chromosome mapping of repetitive sequences in Anostomidae species: implications for genomic and sex chromosome evolution

Molecular Cytogenetics, 2012
Background Members of the Anostomidae family provide an interesting model system for the study of the influence of repetitive elements on genome composition, mainly because they possess numerous heterochromatic segments and a peculiar system of female ...
da Silva Edson Lourenço, de Borba Rafael Splendore, Parise-Maltempi Patrícia Pasquali   +2 more
doaj   +1 more source

Epigenetics and Behçet’s Disease: DNA Methylation Specially Highlighted

Iranian Journal of Allergy, Asthma and Immunology, 2019
Behçet's disease (BD) is a multisystem inflammatory disease with unknown etiology. Although evidence about the pathogenesis of BD is growing, the actual cause of this disease is unclear. Both genetic and epigenetic factors are claimed to play significant
Jafar Farhadi, Mohammad Nouri, Ebrahim Sakhinia, Nasser Samadi, Zohreh Babaloo, Shahriar Alipour, Golamreza Jadideslam, Farhad Pouremamali, Alireza Khabbazi   +8 more
doaj   +1 more source

The association of the interspersed repetitive KpnI sequences with the nuclear matrix.

Journal of Biological Chemistry, 1985
The KpnI sequences constitute the dominant, long, interspersed repetitive DNA families in primate genomes. These families contain related, but nonidentical sequence subsets, some of which border functional gene domains and are transcribed into RNA. To test whether these sequences perform an organizational function in the nucleus, their association with
Chimera, J. A., Musich, Phillip R.
openaire   +2 more sources