Oxidative protein biogenesis and redox regulation in the mitochondrial intermembrane space [PDF]
, 2016 Mitochondria are organelles that play a central role in cellular metabolism, as they are responsible for processes such as iron/sulfur cluster biogenesis, respiration and apoptosis.
MacPherson, Lisa +2 more
core +1 more source
Enantioselective Intramolecular C-H Amination Catalyzed by Engineered Cytochrome P450 Enzymes In Vitro and In Vivo [PDF]
, 2013 Nitrogen activation: Though P450 enzymes are masters of oxygen activation and insertion into C-H bonds, their ability to use nitrogen for the same purpose has so far not been explored.
Arnold, Frances H. +6 more
core +1 more source
Structural basis for the role of Serine-Rich Repeat Proteins from Lactobacillus reuteri in gut microbe-host interactions [PDF]
, 2018 Lactobacillus reuteri, a Gram-positive bacterial species inhabiting the gastrointestinal tract of vertebrates displays remarkable host adaptation. Previous mutational analyses of rodent strain L.
A. Patrick Gunning +19 more
core +1 more source
EC-BLAST: a tool to automatically search and compare enzyme reactions. [PDF]
, 2014 We present EC-BLAST (http://www.ebi.ac.uk/thornton-srv/software/rbl/), an algorithm and Web tool for quantitative similarity searches between enzyme reactions at three levels: bond change, reaction center and reaction structure similarity.
A Dalby +32 more
core +3 more sources
Mechanistic insight into 3-methylmercaptopropionate metabolism and kinetical regulation of demethylation pathway in marine dimethylsulfoniopropionate-catabolizing bacteria [PDF]
, 2019 The vast majority of oceanic dimethylsulfoniopropionate (DMSP) is thought to be catabolized by bacteria via the DMSP demethylation pathway. This pathway contains four enzymes termed DmdA, DmdB, DmdC and DmdD/AcuH, which together catabolise DMSP to ...
Cao, Hai-Yan +12 more
core +1 more source
Structural Enzymology of Cellvibrio japonicus Agd31B Protein Reveals α-Transglucosylase Activity in Glycoside Hydrolase Family 31 [PDF]
, 2012 The metabolism of the storage polysaccharides glycogen and starch is of vital importance to organisms from all domains of life. In bacteria, utilization of these -glucans requires the concerted action of a variety of enzymes, including glycoside ...
Adams +71 more
core +1 more source
The FEBS Journal, EarlyView.Cystathionine β‐synthase (CBS) is an essential enzyme involved in cysteine metabolism. The mutation R336C, associated with severe homocystinuria and of high incidence in Qatar, reduces its activity. Our research shows that this mutation does not destabilize the enzyme fold but subtly alters the tautomeric equilibrium of the PLP cofactor within the ...
Carolina Conter +10 more
wiley +1 more source
Molecular Microbiology, EarlyView.In Hyphomicrobium denitrificans, the use of thiosulfate as a supplemental electron donor is regulated by two homologous sulfane‐sulfur responsive ArsR‐type transcriptional repressors, sHdrR and SoxR, that act cooperatively. Remarkably, the regulation by sHdrR/SoxR extends far beyond sulfur oxidation and deeply affects anaerobic metabolism, particularly
Jingjing Li +3 more
wiley +1 more source
Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native Activity. [PDF]
, 2016 The extent to which an emerging new function trades off with the original function is a key characteristic of the dynamics of enzyme evolution. Various cases of laboratory evolution have unveiled a characteristic trend; a large increase in a new ...
Emond, S +3 more
core +4 more sources
FEBS Letters, Volume 599, Issue 15, Page 2201-2209, August 2025.Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel +2 more
wiley +1 more source