Results 51 to 60 of about 29,414 (258)

Rapid Evolution of Virus Sequences in Intrinsically Disordered Protein Regions [PDF]

PLoS Pathogens, 2014
Nodamura Virus (NoV) is a nodavirus originally isolated from insects that can replicate in a wide variety of hosts, including mammals. Because of their simplicity and ability to replicate in many diverse hosts, NoV, and the Nodaviridae in general, provide a unique window into the evolution of viruses and host-virus interactions. Here we show that the C-
Gitlin, Leonid, Hagai, Tzachi, LaBarbera, Anthony, Solovey, Mark, Andino, Raul   +4 more
openaire   +5 more sources

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Design of intrinsically disordered region binding proteins

Science
Abstract Intrinsically disordered proteins and peptides play key roles in biology, but the lack of defined structures and the high variability in sequence and conformational preferences has made targeting such systems challenging. We describe a general approach for designing proteins that bind intrinsically disordered protein regions in
Kejia Wu, Hanlun Jiang, Derrick R. Hicks, Caixuan Liu, Edin Muratspahić, Theresa A. Ramelot, Yuexuan Liu, Kerrie McNally, Sebastian Kenny, Andrei Mihut, Amit Gaur, Brian Coventry, Wei Chen, Asim K. Bera, Alex Kang, Stacey Gerben, Mila Ya-Lan Lamb, Analisa Murray, Xinting Li, Madison A. Kennedy, Wei Yang, Zihao Song, Gudrun Schober, Stuart M. Brierley, John O’Neill, Michael H. Gelb, Gaetano T. Montelione, Emmanuel Derivery, David Baker   +28 more
openaire   +4 more sources

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Fuzzy recognition by the prokaryotic transcription factor HigA2 from Vibrio cholerae

Nature Communications
Disordered protein sequences can exhibit different binding modes, ranging from well-ordered folding-upon-binding to highly dynamic fuzzy binding. The primary function of the intrinsically disordered region of the antitoxin HigA2 from Vibrio cholerae is ...
San Hadži, Zala Živič, Matic Kovačič, Uroš Zavrtanik, Sarah Haesaerts, Daniel Charlier, Janez Plavec, Alexander N. Volkov, Jurij Lah, Remy Loris   +9 more
doaj   +1 more source

The Disordered C-Terminus of the Chaperone DnaK Increases the Competitive Fitness of Pseudomonas putida and Facilitates the Toxicity of GraT

Microorganisms, 2021
Chaperone proteins are crucial for proper protein folding and quality control, especially when cells encounter stress caused by non-optimal temperatures. DnaK is one of such essential chaperones in bacteria. Although DnaK has been well characterized, the
Sirli Rosendahl, Andres Ainelo, Rita Hõrak   +2 more
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Functional Segments on Intrinsically Disordered Regions in Disease-Related Proteins [PDF]

Biomolecules, 2019
One of the unique characteristics of intrinsically disordered proteins (IPDs) is the existence of functional segments in intrinsically disordered regions (IDRs). A typical function of these segments is binding to partner molecules, such as proteins and DNAs. These segments play important roles in signaling pathways and transcriptional regulation.
Anbo, Hiroto, Sato, Masaya, Okoshi, Atsushi, Fukuchi, Satoshi   +3 more
openaire   +3 more sources

Conformational dynamics in the disordered region of human CPEB3 linked to memory consolidation

BMC Biology, 2022
Background Current understanding of the molecular basis of memory consolidation points to an important function of amyloid formation by neuronal-specific isoforms of the cytoplasmic polyadenylation element binding (CPEB) protein family.
D. Ramírez de Mingo, D. Pantoja-Uceda, R. Hervás, M. Carrión-Vázquez, D. V. Laurents   +4 more
doaj   +1 more source

Disease association and comparative genomics of compositional bias in human proteins [version 2; peer review: 2 approved]

F1000Research, 2023
Background: The evolutionary rate of disordered protein regions varies greatly due to the lack of structural constraints. So far, few studies have investigated the presence/absence patterns of compositional bias, indicative of disorder, across ...
Christos A. Ouzounis, Anastasia Chasapi, Christos E. Kouros, Vasiliki Makri   +3 more
doaj   +1 more source