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Relationship Between Serum Concentration, Functional Parameters and Cell Bioenergetics in IPEC-J2 Cell Line

Histochemistry and Cell Biology, 2021
The foetal bovine serum (FBS) concentration could influence functional parameters of IPEC-J2 cells. IPEC-J2 is a non-transformed continuous epithelial cell line that represents an established in vitro model to study porcine gut inflammation and alterations of intestinal integrity.
Salvatore Nesci, Alessandra Pagliarani, Cristina Algieri   +2 more
exaly   +3 more sources

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Effects of TLR9/NF-κB on oxidative stress and inflammation in IPEC-J2 cells

Genes & Genomics, 2022
Oxidative stress is one of the most important factors affecting large-scale breeding, especially the performance of pigs. Oxidative stress plays a role by affecting various genes in pigs, which can cause serious body damage, functional degradation and reduce production performance.The purpose of this study was to investigate the effect of Toll like ...
Lixia Ma, Jinhong Geng, Wei Chen, Ming Qin, Lixue Wang, Yongqing Zeng   +5 more
openaire   +2 more sources

Transcriptional Profiling of Zearalenone-Induced Inhibition of IPEC-J2 Cell Proliferation

Toxicon, 2019
Mounting evidence has shown that zearalenone (ZEA) can have toxic effects on the intestinal epithelial cells (IECs) of mammals and humans, but the mechanism of ZEA-induced toxicity on IECs is unclear. The aim of this study was to reveal the mechanism of action of ZEA on intestinal epithelial cells via RNA-seq technology.
Yongfeng Zhou, Hao Yu, Yong-Cheng Jin
exaly   +3 more sources

Deoxynivalenol Induced Apoptosis and Inflammation of IPEC-J2 Cells by Promoting ROS Production

Environmental Pollution, 2019
Deoxynivalenol (DON) frequently detected in a wide range of foods and feeds, inducing cytotoxicity to animals and humans. To investigate the underlying mechanism of DON-induced apoptosis and inflammation in porcine small intestinal epithelium, intestinal porcine epithelial cells (IPEC-J2 cells) were chosen as objects, and were treated by different ...
Chunmei Li, Yansen Li, Pengyuan Dai
exaly   +3 more sources

Expression of Selenoprotein Genes Is Affected by Heat Stress in IPEC-J2 Cells

Biological Trace Element Research, 2015
The aim of this study was to explore the impacts of heat stress (HS) on expressions of selenoprotein genes in IPEC-J2 cells. Cells were cultured with 5 % CO2-humidified chamber at 37 °C until the cells grew to complete confluence and then exposed to a mild hyperthermia at 41.5 °C (HS) or 37 °C (control) for another 24 h, finally harvested for total RNA
Lei, Cao, Jiayong, Tang, Qiang, Li, Jingyang, Xu, Gang, Jia, Guangmang, Liu, Xiaoling, Chen, Haiying, Shang, Jingyi, Cai, Hua, Zhao   +9 more
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The Effects of Butyric Acid on the Differentiation, Proliferation, Apoptosis, and Autophagy of IPEC-J2 Cells

Current Molecular Medicine, 2020
Background: Butyric acid (BT), a short-chain fatty acid, is the preferred colonocyte energy source. The effects of BT on the differentiation, proliferation, and apoptosis of small intestinal epithelial cells of piglets and its underlying mechanisms have not been fully elucidated.
Yuan, Yang, Jin, Huang, Jianzhong, Li, Huansheng, Yang, Yulong, Yin   +4 more
openaire   +2 more sources

Protective Effect of Koumine, an Alkaloid From Gelsemium sempervirens, on Injury Induced by H2O2 in IPEC-J2 Cells

International Journal of Molecular Sciences, 2019
Medicinal herbal plants have been commonly used for intervention in different diseases and improvement of health worldwide. Koumine, an alkaloid monomer found abundantly in Gelsemium plants, can be effectively used as an antioxidant.
Chenglin Yang, Jing Wu, Yong Wu
exaly   +2 more sources

Zearalenone induces ROS‐mediated mitochondrial damage in porcine IPEC‐J2 cells

Journal of Biochemical and Molecular Toxicology, 2017
AbstractIn this study, the mitochondrial damage effect and mechanism of zearalenone (ZEA) in swine small intestine IPEC‐J2 cells in vitro were comprehensively characterized. The analyses revealed that ZEA at high doses (8 and 7 μg/mL) can significantly increase P < 0.05 the malondialdehyde levels and decrease antioxidant enzymes activities after 48 ...
Wentao Fan, Tongtong Shen, Qiaoqi Ding, Yanan Lv, Li Li, Kehe Huang, Liping Yan, Suquan Song   +7 more
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Potassium diformate alleviated inflammation of IPEC-J2 cells infected with EHEC

Veterinary Microbiology
Potassium diformate (KDF) is a kind of formate, which possesses the advantages of antimicrobial activity, growth promotion and preventing diarrhea in weaned piglets. However, the researches of KDF in animal production mostly focused on apparent indexes such as growth performance and the mechanisms of KDF on intestinal health have not been reported ...
Ying, Lin, Jun-Lei, Zhai, Ya-Ting, Wang, Ping-Ting, Guo, Jing, Zhang, Chang-Kang, Wang, Ling, Jin, Yu-Yun, Gao   +7 more
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Protective effects of melatonin on deoxynivalenol-induced oxidative stress and autophagy in IPEC-J2 cells

Food and Chemical Toxicology, 2023
This study explored protective effects of melatonin (MEL) on deoxynivalenol (DON)-induced toxicity in porcine jejunum epithelial cells (IPEC-J2). Cells were preexposed to MEL and then exposed to DON to detect cell viability, apoptosis, and oxidative stress indicators.
Yafei Xu, Yunxiao Xie, Zhengchang Wu, Haifei Wang, Zhenhai Chen, Jingneng Wang, Wenbin Bao   +6 more
openaire   +2 more sources