Results 81 to 90 of about 22,432 (259)

A Single-Cell Electroporation Model for Quantitatively Estimating the Pore Area Ratio by High-Frequency Irreversible Electroporation

open access: yesApplied Sciences, 2023
The electroporation technique utilizes pulsed electric fields to induce porous defects in the cell membrane, and the technique can be used for delivering drugs into cells and killing cancer cells.
Lujia Ding   +4 more
doaj   +1 more source

Irreversible electroporation on the small intestine [PDF]

open access: yesBritish Journal of Cancer, 2012
Non-thermal irreversible electroporation (NTIRE) has recently been conceived as a new minimally invasive ablation method, using microsecond electric fields to produce nanoscale defects in the cell membrane bilayer and induce cell death while keeping all other molecules, including the extracellular matrix, intact.
Phillips, MA   +3 more
openaire   +4 more sources

A micro cell lysis device [PDF]

open access: yes, 1998
A new micromachined cell lysis device is developed. It is designed for miniature bio-analysis systems where cell lysing is needed to obtain intracellular materials for further analysis such as DNA identification.
Lee, S. W., Tai, Y. C., Yowanto, H.
core  

The relationship between membrane damage, release of protein and loss of viability in Escherichia coli exposed to high hydrostatic pressure [PDF]

open access: yes, 2010
The aim of this work was to examine a possible association between resistance of two Escherichia coli strains to high hydrostatic pressure and the susceptibility of their cell membranes to pressure-induced damage. Cells were exposed to pressures between
Klotz, Bernadette   +2 more
core   +1 more source

Systematic Metabolic Engineering and Model‐Guided Optimization for High‐Level Production of L‐Theanine from Xylose in Escherichia coli

open access: yesAdvanced Science, EarlyView.
This study is pioneering in constructing the shortest known synthetic pathway for L‐theanine production from xylose within E coli. Through comprehensive metabolic engineering strategies, our engineered strain achieved the highest reported L‐theanine titer from xylose, with a titer of 95.42 g/L, and a yield of 0.55 g/g.
Haolin Han   +5 more
wiley   +1 more source

Engineering Immune Cell to Counteract Aging and Aging‐Associated Diseases

open access: yesAdvanced Science, EarlyView.
This review highlights a paradigm shift in which advanced immune cell therapies, initially developed for cancer, are now being harnessed to combat aging. By engineering immune cells to selectively clear senescent cells and remodel pro‐inflammatory tissue microenvironments, these strategies offer a novel and powerful approach to delay age‐related ...
Jianhua Guo   +5 more
wiley   +1 more source

Oxidative Effects during Irreversible Electroporation of Melanoma Cells—In Vitro Study

open access: yesMolecules, 2020
Irreversible electroporation (IRE) is today used as an alternative to surgery for the excision of cancer lesions. This study aimed to investigate the oxidative and cytotoxic effects the cells undergo during irreversible electroporation using IRE ...
Wojciech Szlasa   +7 more
doaj   +1 more source

Thermal Effects of Irreversible Electroporation

open access: yes, 2017
Although irreversible electroporation (IRE) is thought to be a nonthermal technique for the ablation of soft tissue, objective temperature measurements and mathematical models have shown that temperature increase during treatment can be significant. The nonthermal mechanism is thought to be caused by a disturbance in the cell’s homeostasis through the ...
Eran van Veldhuisen   +3 more
openaire   +1 more source

Basic Features of a Cell Electroporation Model: Illustrative Behavior for Two Very Different Pulses [PDF]

open access: yes, 2014
Science increasingly involves complex modeling. Here we describe a model for cell electroporation in which membrane properties are dynamically modified by poration.
Gowrishankar, Thiruvallur R.   +4 more
core   +4 more sources

Unsaturated Phosphorus Electrophiles to Probe Protein Tyrosine Phosphatases

open access: yesAngewandte Chemie International Edition, EarlyView.
Charged aryl‐ethynyl phosphonamidic and phosphonic acids are introduced as low‐reactivity electrophiles for peptide‐based activity probes that enable selective, target‐specific profiling of protein tyrosine phosphatases. The probes show no off‐target cysteine reactivity and engage only the interacting phosphatase in global proteomic analysis.
Eleftheria Poulou   +4 more
wiley   +1 more source

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