A Mini Review on Capillary Isoelectric Focusing-Mass Spectrometry for Top-Down Proteomics [PDF]
Frontiers in Chemistry, 2021 Mass spectrometry (MS)-based top-down proteomics (TDP) requires high-resolution separation of proteoforms before electrospray ionization (ESI)-MS and tandem mass spectrometry (MS/MS).
Tian Xu, Liangliang Sun
doaj +2 more sources
SUBTYPE DETERMINATION OF PHOSPHOGLUCOMUTASE (PGM) AND GROUP- SPECIFIC COMPONENT (Gc) BY ISOELECTRIC FOCUSING AMONG SOME IRANIAN POPULATIONS [PDF]
Iranian Journal of Public Health, 1996 A total of 781 serum and 827 red cell samples from four populations of Iran (Kashan, Ardestan, Asrara and Ardabil) were sub-typed by isoelectric focusing for group-specific componet and red cell enzyme Phosphoglucomutase, locus one, respectively ...
S.Y. Seyedna
doaj +2 more sources
Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina [PDF]
Bio-Protocol, 2019 Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, reduces its
Sowmya Bekshe Lokappa +2 more
doaj +2 more sources
Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping [PDF]
Practical Laboratory Medicine, 2020 A qualitative, semi-automatized method for apolipoprotein E (apoE) phenotyping by isoelectric focusing method has been evaluated on 40 serum samples from patients previously genotyped for apoE, especially as regards concordance with genotyping, but also ...
Randa Bittar +7 more
doaj +2 more sources
Exploring imaged capillary isoelectric focusing parameters for enhanced charge variants quality control [PDF]
Frontiers in ChemistryBiopharmaceuticals are increasingly utilised in the treatment of oncological, inflammatory, and autoimmune diseases, largely due to their exceptional specificity in targeting antigens.
Virginia Ghizzani +5 more
doaj +2 more sources
Isoelectric focusing of proteins in the pH gradient as a tool for identification of species origin of raw meat [PDF]
Journal of Veterinary Research, 2018 Health, religious, and commercial aspects justify the need for meat species identification. The lack of officially approved methods prompts the undertaking of research on validation of isoelectric focusing of proteins (IEF) for official purposes.
Różycki Mirosław +4 more
doaj +2 more sources
Detecting peroxiredoxin hyperoxidation by one-dimensional isoelectric focusing. [PDF]
Biophys Rep, 2015 The activity of typical 2-cys peroxiredoxin (Prxs) can be regulated by hyperoxidation with a consequent loss of redox activity. Here we developed a simple assay to monitor the level of hyperoxidation of different typical 2-cys prxs simultaneously.
Cao Z, Bulleid NJ.
europepmc +3 more sources
Capillary Isoelectric Focusing Immunoassay for Fat Cell Differentiation Proteomics. [PDF]
PLoS ONE, 2015 Profiling cellular proteome is critical to understanding signal integration during cell fate determination. In this study, the capability of capillary isoelectric focusing (cIEF) immunoassays to detect post-translational modifications (PTM) of protein ...
Mary G Johlfs +4 more
doaj +2 more sources
International Journal of Horticultural Science, 2005 Self-incompatibility (SI) in flowering plants is a widespread genetic system that promotes out-crossing. In Prunus species the SI is a gametophytic trait, which is controlled by a single multiallelic locus, termed S-locus.
A. Pedryc +3 more
doaj +3 more sources
Erratum: Comparison of two different canine anti-IgG antibodies for assessment of oligoclonal bands in cerebrospinal fluid and serum of dogs via isoelectric focusing followed by an immunoblot. [PDF]
Front Vet Sci, 2023 Frontiers Production Office.
europepmc +3 more sources