A Mini Review on Capillary Isoelectric Focusing-Mass Spectrometry for Top-Down Proteomics [PDF]
Frontiers in Chemistry, 2021 Mass spectrometry (MS)-based top-down proteomics (TDP) requires high-resolution separation of proteoforms before electrospray ionization (ESI)-MS and tandem mass spectrometry (MS/MS).
Tian Xu, Liangliang Sun
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Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina [PDF]
Bio-Protocol, 2019 Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, reduces its
Sowmya Bekshe Lokappa +2 more
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SUBTYPE DETERMINATION OF PHOSPHOGLUCOMUTASE (PGM) AND GROUP- SPECIFIC COMPONENT (Gc) BY ISOELECTRIC FOCUSING AMONG SOME IRANIAN POPULATIONS [PDF]
Iranian Journal of Public Health, 1996 A total of 781 serum and 827 red cell samples from four populations of Iran (Kashan, Ardestan, Asrara and Ardabil) were sub-typed by isoelectric focusing for group-specific componet and red cell enzyme Phosphoglucomutase, locus one, respectively ...
S.Y. Seyedna
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Exploring imaged capillary isoelectric focusing parameters for enhanced charge variants quality control [PDF]
Frontiers in ChemistryBiopharmaceuticals are increasingly utilised in the treatment of oncological, inflammatory, and autoimmune diseases, largely due to their exceptional specificity in targeting antigens.
Virginia Ghizzani +5 more
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Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping [PDF]
Practical Laboratory Medicine, 2020 A qualitative, semi-automatized method for apolipoprotein E (apoE) phenotyping by isoelectric focusing method has been evaluated on 40 serum samples from patients previously genotyped for apoE, especially as regards concordance with genotyping, but also ...
Randa Bittar +7 more
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Isoelectric focusing of proteins in the pH gradient as a tool for identification of species origin of raw meat [PDF]
Journal of Veterinary Research, 2018 Health, religious, and commercial aspects justify the need for meat species identification. The lack of officially approved methods prompts the undertaking of research on validation of isoelectric focusing of proteins (IEF) for official purposes.
Różycki Mirosław +4 more
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Capillary Isoelectric Focusing Immunoassay for Fat Cell Differentiation Proteomics. [PDF]
PLoS ONE, 2015 Profiling cellular proteome is critical to understanding signal integration during cell fate determination. In this study, the capability of capillary isoelectric focusing (cIEF) immunoassays to detect post-translational modifications (PTM) of protein ...
Mary G Johlfs +4 more
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International Journal of Horticultural Science, 2005 Self-incompatibility (SI) in flowering plants is a widespread genetic system that promotes out-crossing. In Prunus species the SI is a gametophytic trait, which is controlled by a single multiallelic locus, termed S-locus.
A. Pedryc +3 more
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Highly sensitive and specific protein detection via combined capillary isoelectric focusing and proximity ligation [PDF]
Scientific Reports, 2017 Detection and quantification of proteins and their post-translational modifications are crucial to decipher functions of complex protein networks in cell biology and medicine.
Narendra Padhan +9 more
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Erratum: Comparison of two different canine anti-IgG antibodies for assessment of oligoclonal bands in cerebrospinal fluid and serum of dogs via isoelectric focusing followed by an immunoblot. [PDF]
Front Vet Sci, 2023 Frontiers Production Office.
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