A Mini Review on Capillary Isoelectric Focusing-Mass Spectrometry for Top-Down Proteomics [PDF]
Frontiers in Chemistry, 2021 Mass spectrometry (MS)-based top-down proteomics (TDP) requires high-resolution separation of proteoforms before electrospray ionization (ESI)-MS and tandem mass spectrometry (MS/MS).
Tian Xu, Liangliang Sun
doaj +2 more sources
Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina [PDF]
Bio-Protocol, 2019 Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, reduces its
Sowmya Bekshe Lokappa +2 more
doaj +2 more sources
Exploring imaged capillary isoelectric focusing parameters for enhanced charge variants quality control [PDF]
Frontiers in ChemistryBiopharmaceuticals are increasingly utilised in the treatment of oncological, inflammatory, and autoimmune diseases, largely due to their exceptional specificity in targeting antigens.
Virginia Ghizzani +5 more
doaj +2 more sources
Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping [PDF]
Practical Laboratory Medicine, 2020 A qualitative, semi-automatized method for apolipoprotein E (apoE) phenotyping by isoelectric focusing method has been evaluated on 40 serum samples from patients previously genotyped for apoE, especially as regards concordance with genotyping, but also ...
Randa Bittar +7 more
doaj +2 more sources
Isoelectric focusing of proteins in the pH gradient as a tool for identification of species origin of raw meat [PDF]
Journal of Veterinary Research, 2018 Health, religious, and commercial aspects justify the need for meat species identification. The lack of officially approved methods prompts the undertaking of research on validation of isoelectric focusing of proteins (IEF) for official purposes.
Różycki Mirosław +4 more
doaj +2 more sources
Capillary Isoelectric Focusing Immunoassay for Fat Cell Differentiation Proteomics. [PDF]
PLoS ONE, 2015 Profiling cellular proteome is critical to understanding signal integration during cell fate determination. In this study, the capability of capillary isoelectric focusing (cIEF) immunoassays to detect post-translational modifications (PTM) of protein ...
Mary G Johlfs +4 more
doaj +2 more sources
Highly sensitive and specific protein detection via combined capillary isoelectric focusing and proximity ligation [PDF]
Scientific Reports, 2017 Detection and quantification of proteins and their post-translational modifications are crucial to decipher functions of complex protein networks in cell biology and medicine.
Narendra Padhan +9 more
doaj +2 more sources
Microchip Isoelectric Focusing Applications
CHIMIA, 2008 This review summarizes all available publications on isoelectric focusing carried out on planar microfabricated devices. Characteristic features of microchip isoelectric focusing (MC-IEF), such as resolution independence of separation length and
Markéta Vlčková, Maria A. Schwarz
doaj +1 more source
Reduction and Alkylation of Proteins in 2D Gel Electrophoresis: Before or after Isoelectric Focusing? [PDF]
Frontiers in Chemistry, 2017 Xiaolin Wu, Chenhui Xu, Wei Wang
doaj +2 more sources
Isoelectric Point Separations of Peptides and Proteins
Proteomes, 2017 The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection.
Melissa R. Pergande +1 more
doaj +1 more source