Highly sensitive and specific protein detection via combined capillary isoelectric focusing and proximity ligation [PDF]
Detection and quantification of proteins and their post-translational modifications are crucial to decipher functions of complex protein networks in cell biology and medicine.
Narendra Padhan +9 more
doaj +2 more sources
Transferrin isoelectric focusing for the investigation of congenital disorders of glycosylation: analysis of a ten-year experience in a Brazilian center. [PDF]
Magalhães APPS +7 more
europepmc +2 more sources
Reducing Cathodic Drift during Isoelectric Focusing Using Microscale Immobilized pH Gradient Gels. [PDF]
Lomeli G, Herr AE.
europepmc +3 more sources
Inkjet printed silver electrodes on macroporous paper for a paper-based isoelectric focusing device. [PDF]
Gaspar C +3 more
europepmc +3 more sources
Reduction and Alkylation of Proteins in 2D Gel Electrophoresis: Before or after Isoelectric Focusing? [PDF]
Xiaolin Wu, Chenhui Xu, Wei Wang
doaj +2 more sources
Microchip Isoelectric Focusing Applications
This review summarizes all available publications on isoelectric focusing carried out on planar microfabricated devices. Characteristic features of microchip isoelectric focusing (MC-IEF), such as resolution independence of separation length and
Markéta Vlčková, Maria A. Schwarz
doaj +1 more source
Multiple sclerosis in Brazil: analysis of cerebrospinal fluid by standard methods [PDF]
The demonstration of intrathecal IgG synthesis has been used as an important laboratory parameter to support the diagnosis of multiple sclerosis(MS). The Committee for European Concerted Action for Multiple Sclerosis has recommended a protocol for the ...
MARZIA PUCCIONI-SOHLER +4 more
doaj +1 more source
Isoelectric Point Separations of Peptides and Proteins
The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection.
Melissa R. Pergande +1 more
doaj +1 more source
Hemoglobinopathies are inherited red blood cell disorders. They are commonly screened from newborns early after birth, and if not detected early and treated properly, some of them can be fatal.
Santeri Mantyniemi +2 more
doaj +1 more source
Detergents and Chaotropes for Protein Solubilization before Two-Dimensional Electrophoresis [PDF]
Because of the outstanding separating capabilities of two-dimensional electrophoresis for complete proteins, it would be advantageous to be able to apply it to all types of proteins.
Chevallet, Mireille +3 more
core +4 more sources

