Multidimensional separation prior to mass spectrometry: Getting closer to the bottom of the iceberg [PDF]
, 2013 While prefractionation has previously been shown to improve results in MS analysis, a novel combination provides an additional dimension of separation: protein fractionation by SDS-PAGE followed by IEF of tryptic peptides before separation by RP-LC ...
Mayr, Manuel, Rabilloud, Thierry
core +4 more sources
OENO One, 1996 In order ta estimate c1arifying aptitude and the effects of prefermentation treatment by commercial pectinases, polygalacturonase (PG), pectin Iyase (PL), pectinmethylesterase (PME) and proteolytic activities of six pectinases samples were studied.
Laurent Berthier +2 more
doaj +1 more source
Sensitivity and specificity of detection methods for erythropoietin doping in cyclists [PDF]
, 2019 Recombinant human erythropoietin (rHuEPO) is used as doping a substance. Anti-doping efforts include urine and blood testing and monitoring the athlete biological passport (ABP).
Burggraaf, Jacobus +10 more
core +1 more source
Influence of extraction method on protein profile of soybeans [PDF]
Hemijska Industrija, 2013 Comparison between protein profiles of soybean obtained by commonly used methods of extraction (Tris buffer and Tris-urea buffer) with methods used for extraction of plant proteins for 2D PAGE analysis (direct solubilization in IEF buffer, acetone ...
Pavlićević Milica Ž. +2 more
doaj +1 more source
Endogenous mammalian lectin localized extracellularly in lung elastic fibers. [PDF]
, 1984 An affinity-purified antibody preparation raised against a beta-galactoside-binding lectin from bovine lung was used to localize a similar lectin in rat lung by immunofluorescence and by electron microscopy after on-grid staining visualized with ...
Barondes, SH +2 more
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Validation of isoelectric focusing method for recombinant erythropoietin detection in human urine
Saúde & Tecnologia, 2015 Erythropoietin (EPO) is a substance that stimulates red blood cell production, increasing muscle oxygenation and is secreted naturally by the body and excreted in urine in low concentrations.
Ana Sofia Rodrigues Tavares
doaj +1 more source
Detecting β-Casein Variation in Bovine Milk
Molecules, 2016 In bovine species, β-casein (β-CN) is characterized by genetic polymorphism. The two most common protein variants are β-CN A2 (the original one) and A1, differing from A2 for one amino acid substitution (Pro67 to His67).
Anna Maria Caroli +3 more
doaj +1 more source
Biomolecules, 2022 Congenital Disorders of Glycosylation (CDG) are multisystemic metabolic disorders showing highly heterogeneous clinical presentation, molecular etiology, and laboratory results.
Karolina Mitusińska +5 more
doaj +1 more source
Variation in the Thyrotropic Activity of Human Chorionic Gonadotropin in Chinese Hamster Ovary Cells Arises from Differential Expression of the Human Thyrotropin Receptor and Microheterogeneity of the Hormone. [PDF]
, 1995 The role of hCG as a stimulator of the human thyroid has been a subject of controversy, because discrepant results have been obtained in different in vitro assays.
Amir, S.M. +4 more
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Effects of Biological Sex and Age on Cerebrospinal Fluid Markers—A Retrospective Observational Study
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective Cerebrospinal fluid (CSF) analysis is a key diagnostic tool for neurological diseases. To date, only a few studies have investigated in larger cohorts the effect of age and biological sex on diagnostic markers extracted from CSF. Methods For this retrospective observational study, 4163 CSF findings (2012–2020) were evaluated.
Isabel‐Sophie Hafer +3 more
wiley +1 more source